Type-specific identification of influenza viruses A, B and C by the polymerase chain reaction

Claas, Eric C. J.; Sprenger, M. J. W.; Kletera, G.E.M.; Beek, Ruud van; Quint, Wim; Masurela, N.

doi:10.1016/0166-0934(92)90120-3

Cited by 94 publications

(53 citation statements)

References 23 publications

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“…A transcrição reversa foi realizada com o kit High Capacity cDNA Archive (Applied Biosystems), conforme as instruções do fabricante. Para a amplificação das amostras, foi utilizado o método de reação em cadeia da polimerase (polymerase chain reaction, PCR) com primers específicos para a detecção de VSRH, MPVh, IA, IB, VPI-1, VPI-2, VPI-3 e picornavírus [11][12][13][14] .…”

Section: Detecção Molecular Viralunclassified

Frequent respiratory pathogens of respiratory tract infections in children attending daycare centers

Bonfim¹,

Nogueira²,

Simas³

et al. 2011

J Pediatr (Rio J)

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Objectives: To identify and characterize respiratory viruses that infect children from daycare centers with symptoms of respiratory infection and to evaluate the association of clinical and epidemiological disease data with the identified virus. Methods:We conducted a study between 2003 and 2005 in 176 children with respiratory infection symptoms attending a municipal daycare center. Samples from nasopharyngeal secretion were tested by reverse transcriptase polymerase chain reaction and positive samples for picornavirus were sequenced.Results: All 782 collected samples were analyzed and 31.8% were positive for at least one of the studied respiratory viruses. Respiratory infections were characterized by the presence of mild symptoms of the upper respiratory tract, the most common of which were runny nose and cough. In the 2 years of study, most cases of infection occurred in autumn and winter, but respiratory viruses were detected throughout all the study period. Conclusions:Respiratory viruses and respiratory infections caused by them are part of the daily life of children attending daycare centers. Our results show the great impact that respiratory infections have on these children and suggest that more attention must be paid to viral pathogens. Resultados: Todas as 782 amostras coletadas foram analisadas, e 31,8% foram positivas para algum dos vírus respiratórios estudados. As infecções respiratórias foram caracterizadas pela presença de sintomas moderados do trato respiratório superior, sendo os mais comuns coriza e tosse. Em 2 anos de estudo, a maioria dos casos de infecção ocorreram no outono e no inverno, mas vírus respiratórios foram detectados ao longo de todo o período de estudo. J Pediatr (Rio J) Conclusões:Vírus respiratórios e as infecções respiratórias causadas por eles fazem parte do cotidiano de crianças que frequentam creches. Nossos resultados mostram o grande impacto gerado pelas infecções respiratórias nessas crianças e sugere que uma maior atenção deve ser dispensada aos patógenos virais.J Pediatr (Rio J). 2011;87(5):439-44: Vírus respiratórios, infecção respiratória aguda, crianças de creche.

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Section: Detecção Molecular Viralunclassified

Frequent respiratory pathogens of respiratory tract infections in children attending daycare centers

Bonfim¹,

Nogueira²,

Simas³

et al. 2011

J Pediatr (Rio J)

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“…The two hMPV RT-PCRs were integrated in the existing in-house RT-multiplex PCR as follows: primers for the hMPV N gene were combined with those for RSV, influenza viruses A and B (block 1), and primers for HMPV L gene were combined with those for adenoviruses and parainfluenza type 1 and type 3 viruses (block 2) [6,13,20,26,38]. RNA of RNA viruses was transcribed to cDNA before PCR was performed.…”

Section: Laboratory Investigationsmentioning

confidence: 99%

Clinical findings and unusual epidemiologic characteristics of human metapneumovirus infections in children in the region of Basel, Switzerland

2007

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Human metapneumovirus (hMPV) worldwide causes respiratory tract infections with features similar to those of RSV infection. We describe features of hMPV infections in children and compare some of the characteristics with those of RSV infections. From October 2004 to February 2006, 75 patients, 34 hospitalized and 41 outpatients, were diagnosed with hMPV infections by multiplex PCR applied to nasopharyngeal specimens. While hMPV was found rarely in the early phase of the study, a significant increase occurred in the second winter of the study period. Patients with hMPV infections were older than those with RSV infection; clinical characteristics were similar as was the rate of serious disease among hospitalized patients (intensive care treatment: 18% versus 8%). In conclusion, hMPV leads to endemic and epidemic respiratory disease with features similar to those of RSV and should be considered in the differential diagnosis of upper and lower respiratory tract disease.

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“…There have been some reports on the use of the RT-PCR method to detect the influenza C virus (3,4,24), but there have been few reports on its actual use in the surveillance of this virus. In 2004, more than 70 strains of influenza C virus were isolated in Yamagata and Miyagi Prefectures, and we wanted to learn whether an epidemic of influenza C had occurred on a national scale.…”

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confidence: 99%

A Nationwide Epidemic of Influenza C Virus Infection in Japan in 2004

et al. 2007

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a total of 131 influenza C viruses were detected by cell culture or reverse transcription-PCR (RT-PCR) from specimens that were obtained from children with acute respiratory symptoms in 10 prefectures across Japan. Influenza C virus was identified most frequently in the Miyagi (1.4%, 45 of 3,226 specimens) and Yamagata (2.5%, 31 of 1,263 specimens) prefectures, and the frequency in this year was the highest since 1990. Phylogenetic analysis of the hemagglutinin esterase gene of the 13 strains isolated in nine prefectures revealed that genetically similar strains belonging to the Kanagawa/1/76-related lineage dominantly spread throughout Japan. During the 2004 influenza season, influenza C virus coexisted with epidemics of influenza A virus (H3 strain), and 12 cases were identified from patients who had been diagnosed with influenza-like illness (7 were detected by RT-PCR, and 5 were detected by culture). A comparison of specimens that were found positive by culture with those found positive only by RT-PCR shows that the amount of virus in PCR-positive specimens tended to be lower than in isolation-positive specimens. Although the mean peak temperature in patients in the PCR-positive group was slightly lower, there were no significant differences in characteristics between specimens (i.e., kind of specimen, period from onset to specimen collection, age distribution of patients, and severity of illness). These results suggest that an epidemic of influenza C virus occurred on a national scale during this period and that RT-PCR can be an effective supplemental tool for the evaluation of clinical and epidemiological information.

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Type-specific identification of influenza viruses A, B and C by the polymerase chain reaction

Cited by 94 publications

References 23 publications

Frequent respiratory pathogens of respiratory tract infections in children attending daycare centers

Frequent respiratory pathogens of respiratory tract infections in children attending daycare centers

Clinical findings and unusual epidemiologic characteristics of human metapneumovirus infections in children in the region of Basel, Switzerland

A Nationwide Epidemic of Influenza C Virus Infection in Japan in 2004

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