Types and concentrations of cytokinins in the micropropagation of Anthurium maricense

Abstract

Faced with the demand for plants potted for their foliage, Anthurium maricense is seen as a viable option. However, most of the studies on obtaining micropropagated plantlets are for A. andraeanum, with nothing yet reported for A. maricense. The aim of this study therefore, was to evaluate the effect of four cytokinins in different concentrations, on the in vitro induction of shoots from nodal segments of A. maricense. The experimental design was completely randomised in a 4 x 4 factorial scheme, with four cytokinins (BAP, ZEA, CIN and 2iP) and 4 concentrations (0, 2.22, 4.44 and 6.66 μM), for a total of 16 treatments, with 6 replications of five test tubes, and using one nodal segment. Cultures were kept in a growth room at 25 ± 2°C, a photoperiod of 16 h and a light intensity of 30 μmolm-2 s-1 for 60 days. After this period, the number of shoots formed per node was evaluated. The addition of a cytokinin to the culture medium was determinant for the in vitro regeneration of shoots in A. maricense. The greatest estimated number of shoot formations in A. maricense were obtained in the culture media containing ZEA (3.87) and BAP (3.30), both at concentration of 6.66 μM.

Regeneración y enraizamiento de brotes adventicios etiolados de cultivares de zarzamora (Rubus sp)

“…Castilla y Tupy sin el uso de reguladores de crecimiento y en menor tiempo. Sin embargo, existen reportes de regeneración de brotes adventicios en otras especies donde se emplea mayor periodo de tiempo, como los reportados en brotes de la seleção IAPAR8 que requiere 30 días (Oliveira-Cauduro et al, 2016); y brotes etiolados de Anthurium marícense se obtienen en 60 días (Serafim et al, 2018).…”