ACLIMATIZAÇÃO DE MUDAS MICROPROPAGADAS DE Anthurium maricense COM DIFERENTES LÂMINAS DE IRRIGAÇÃO     ARLENE SANTISTEBAN CAMPOS1; GUILHERME VIEIRA DO BOMFIM2; ANA CRISTINA PORTUGAL PINTO DE CARVALHO3; BENITO MOREIRA DE AZEVEDO2; ANA CECÍLIA RIBEIRO DE CASTRO3 E DENISE VIEIRA VASCONCELOS4   1Departamento de ciência do solo, Universidade Federal do Ceará, Av. Mister Hull, 2977, Campus do Pici, CEP: 60356-001, Fortaleza, Ceará, Brasil, arlenesan@yahoo.com.br. 2Departamento de engenharia agrícola, Universidade Federal do Ceará, Av. Mister Hull, 2977, Campus do Pici, CEP: 60356-001, Fortaleza, Ceará, Brasil, guile2007@gmail.com, benito@ufc.br. 3Embrapa Agroindústria Tropical, Rua Pernambuco, 2270, Pici, CEP 60511-110, Fortaleza, Ceará, Brasil, cristina.carvalho@embrapa.br, cecilia.castro@embrapa.br. 4Instituto Federal de Educação, Ciência e Tecnologia do Pará, Estrada de Ferro de Bragança, s/nº, Taíra, CEP: 68600-000, Bragança, Pará, Brasil, denisevasconcelos@hotmail.com.     1 RESUMO   O objetivo deste trabalho foi avaliar a influência de diferentes lâminas de irrigação na aclimatização de mudas micropropagadas de Anthurium maricense. O delineamento experimental foi o completamente ao acaso, composto por cinco tratamentos e quatro repetições de quatro mudas cada. Os tratamentos foram as lâminas de irrigação estimadas com cinco percentuais da capacidade de retenção de água (CRA) do substrato: 50; 75; 100; 125; e 150% da CRA. As mudas foram cultivadas individualmente em vasos de 415 mL contendo o substrato comercial HS Flores®. Durante a aclimatização, as mudas foram irrigadas duas vezes ao dia e receberam adubações foliares em dias alternados. Foram analisadas as variáveis: incremento na altura da muda (IAM); incremento no número de folhas por muda (INF); incremento na área da maior folha (IAMF); ocupação de vaso (OV); taxa de fotossíntese líquida (A); carbono interno (Ci); e temperatura (Ti) e umidade (Ui) foliar. Com base nos resultados, supõe-se que as mudas de A. maricense possam apresentar evidência de resistência ao déficit hídrico. A lâmina de irrigação estimada com 150% da CRA proporciona o maior valor de IAMF. Lâminas de irrigação intermediárias, entre 100 e 125% da CRA, proporcionam os maiores valores de IAM, INF e OV.   Palavras-chave: antúrio, plantas ornamentais, manejo da irrigação.     CAMPOS, A. S.; BOMFIM, G. V. do; CARVALHO, A. C. P. P. de; AZEVEDO, B. M. de; CASTRO, A. C. R. de; VASCONCELOS, D. V. ACLIMATIZATION OF MICROPROPAGATE PLANTLETS OF Anthurium maricense WITH DIFFERENT IRRIGATION DEPHTS     2 ABSTRACT   The aim of this study was to evaluate the development of micropropagated plantlets of Anthurium maricense during the acclimatization phase in screen environment, under different irrigation depths based on percentages of water retention capacity (WRC), on the weather conditions in the greenhouse environment. The experimental design was completely randomized, composed of five irrigation depths, with four replicates and four plantlets per plot. The treatments consisted of irrigation depths estimated with five levels of water retention capacity (WRC) of the substrate: 50; 75; 100; 125; and 150% of WRC. The plantlets were cultivated in 415 mL pots with HS Flowers® substrate. During the acclimatization, all plantlets were irrigated twice a day and fertilized, on alternate days, with leaf fertilization. The variables analyzed were: plant height increment (PHI); number of leaves increment (NLI); largest leaf area increment (LLAI); pot occupation (PO); net photosynthetic rate (A); internal carbon (Ci); leaf temperature (Ti) and leaf moisture (Ui). Based on the results obtained, it is assumed that A. maricense micropropagated plantlets may present evidence of resistance to water deficit. The estimated irrigation depth of 150% of WRC provides the highest value of LLAV. Intermediate irrigation depth, between 100 and 125% of the WRC, provide the highest values of PHV, NLV and PO.   Keywords: anthurium, ornamental plant, irrigation management.

Faced with the demand for plants potted for their foliage, Anthurium maricense is seen as a viable option. However, most of the studies on obtaining micropropagated plantlets are for A. andraeanum, with nothing yet reported for A. maricense. The aim of this study therefore, was to evaluate the effect of four cytokinins in different concentrations, on the in vitro induction of shoots from nodal segments of A. maricense. The experimental design was completely randomised in a 4 x 4 factorial scheme, with four cytokinins (BAP, ZEA, CIN and 2iP) and 4 concentrations (0, 2.22, 4.44 and 6.66 μM), for a total of 16 treatments, with 6 replications of five test tubes, and using one nodal segment. Cultures were kept in a growth room at 25 ± 2°C, a photoperiod of 16 h and a light intensity of 30 μmolm-2 s-1 for 60 days. After this period, the number of shoots formed per node was evaluated. The addition of a cytokinin to the culture medium was determinant for the in vitro regeneration of shoots in A. maricense. The greatest estimated number of shoot formations in A. maricense were obtained in the culture media containing ZEA (3.87) and BAP (3.30), both at concentration of 6.66 μM.

Anthurium maricense is a tropical ornamental species with great commercial potential in the areas of landscaping and potted foliage plants. One of the main obstacles in the production of micropropagated anthurium plantlets is its slow development and/or the low survival rate of the plantlets during the acclimatization stage. The use of slow-release fertilisers (SRF) can accelerate this process; however, there are no reports in the literature concerning this practice during acclimatization of micropropagated plantlets of this species. As such, in the present study, the effects of different doses of SRF on the development of micropropagated plantlets of Anthurium maricense were evaluated during the acclimatization stage in a screenhouse. The experiment was carried out in a completely randomised design, with five treatments and four replications of four plantlets per plot. The treatments comprised doses of 0.0, 2.5, 5.0, 7.5 and 10.0 kg m-3 of the SRF Osmocote® (NPK 15:9:12) added to the substrate. The variables under evaluation were the variation in plant height, in the number of leaves and in the area of the largest leaf, pot occupancy, net photosynthetic rate, internal carbon, and leaf temperature and moisture. The use of SRF promoted better development of the micropropagated plantlets of A. maricense during the acclimatization stage. Of the variables under analysis, the variation in the area of the largest leaf is the most important, since the commercial interest of the studied species is the foliage, which was at its maximum with an estimated dose of Osmocote® of 6.40 kg m-3.

Anthurium is one of the most marketed species in international floriculture. The availability of sufficient plants of phytosanitary quality is a limiting factor in their commercial cultivation. Micropropagation is the most common technique used in producing plantlets on a large scale. The aim of this study was to determine the most suitable relationship between growth medium, BAP concentration and photoperiod for increasing the in vitro multiplication rate of the ‘Eidibel’ cultivar. A completely randomised design was used in a 2 x 9 x 2 factorial scheme with 20 replications. The treatments comprised combinations of two types of growth medium (MS and Pierik), nine concentrations of BAP (0.0; 1.11; 2.22; 3.33; 4.44; 5.55; 6.66; 7.77 and 8.88 μM), and two photoperiods (12 and 16 h). The number of shoots per explant (NSE) and percentage of explants forming organogenic calli (FOC) and roots (FR) were evaluated after 60 days. The highest values for NSE, 3.66 and 4.69, were obtained in the MS and Pierik growth media, containing 8.88 μM and 4.85 μM BAP respectively. The NSE was higher under the 12-hour regime in the MS medium, and equal under the two photoperiods in the Pierik medium. The lower the BAP concentration, the greater the percentage of root formation and the smaller the percentage of organogenic calli. The Pierik medium is recommended for the production of micropropagated ‘Eidibel’ plantlets, with the addition of 4.85 μM BAP, under a photoperiod of 12 h.

The objective of the present research was to develop a protocol for micropropagation of Anthurium bonplandii and Anthurium maricense by direct organogenesis. Nodal segments, with two or three nodes, were used as explants. The cultures were kept in a growth chamber at a temperature of 25±2ºC, under a photoperiod of 16 hours and a luminosity of 30μmol m-2 s-1. At 60 days, the number of regenerated buds per explant (NBE) was counted. The experiment was carried out in an entirely randomised design consisting of six treatments for six different concentrations of 6-benzylaminopurine (6-BA) added to the P2 (Pierik) medium (0.0, 1.11, 2.22, 3.33, 4.44, and 5.55µM). All the treatments were performed in four repetitions with 10 culture tubes containing one explant each. The regression analyses were adjusted to a quadratic model, with R2 = 88.7% and 62.4% for A. maricense and A. bonplandii, respectively. The regressions indicate that the addition of 6-BA to the P2 medium resulted in larger values of NBE in both the species. The ideal 6-BA concentration for micropropagation varied depending on the species, with 2.5 and 1.7 NBE determined at 6-BA concentrations of 4.70 and 3.37µM for A. maricense and A. bonplandii, respectively.