Types and Prevalence of Carbapenem-Resistant Acinetobacter calcoaceticus-Acinetobacter baumannii Complex in Northern Taiwan

Hsieh, Wen Shyang; Wang, Nai Yu; Feng, Jou An; Weng, Li Chuan; Wu, Hsueh Hsia

doi:10.1128/aac.00779-13

Cited by 19 publications

(7 citation statements)

References 18 publications

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“…Carbapenem resistance is high and the clinician is left with very little choice of antibiotics. The only antibiotic that still retains activity is tigecycline, an observation that has also been made by other authors (Golanbar et al, 2011 ; Hsieh et al, 2014 ). In this unit, piperacillin/tazobactum and amikacin are being used as a pre-emptive (first line) therapy for clinically suspected cases of sepsis.…”

Section: Discussionsupporting

confidence: 63%

Carbapenem Resistance in Acinetobacter baumannii and Other Acinetobacter spp. Causing Neonatal Sepsis: Focus on NDM-1 and Its Linkage to ISAba125

Chatterjee¹,

Datta²,

Roy³

et al. 2016

Front. Microbiol.

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Carbapenem-resistant determinants and their surrounding genetic structure were studied in Acinetobacter spp. from neonatal sepsis cases collected over 7 years at a tertiary care hospital. Acinetobacter spp. (n = 68) were identified by ARDRA followed by susceptibility tests. Oxacillinases, metallo-β-lactamases (MBLs), extended-spectrum β-lactamases and AmpCs, were detected phenotypically and/or by PCR followed by DNA sequencing. Transconjugants possessing the blaNDM−1(New Delhi metallo-β-lactamase) underwent further analysis for plasmids, integrons and associated genes. Genetic environment of the carbapenemases were studied by PCR mapping and DNA sequencing. Multivariate logistic regression was used to identify risk factors for sepsis caused by NDM-1-harboring organisms. A. baumannii (72%) was the predominant species followed by A. calcoaceticus (10%), A. lwoffii (6%), A. nosocomialis (3%), A. junni (3%), A. variabilis (3%), A. haemolyticus (2%), and 14TU (2%). Fifty six percent of the isolates were meropenem-resistant. Oxacillinases present were OXA-23-like, OXA-58-like and OXA-51-like, predominately in A. baumannii. NDM-1 was the dominant MBL (22%) across different Acinetobacter spp. Isolates harboring NDM-1 also possessed bla(VIM−2, PER−1, VEB−2, CTX−M−15), armA, aac(6′)Ib, aac(6′)Ib-cr genes. blaNDM−1was organized in a composite transposon between two copies of ISAba125 in the isolates irrespective of the species. Further, OXA-23-like gene and OXA-58-like genes were linked with ISAba1 and ISAba3 respectively. Isolates were clonally diverse. Integrons were variable in sequence but not associated with carbapenem resistance. Most commonly found genes in the 5′ and 3′conserved segment were aminoglycoside resistance genes (aadB, aadA2, aac4′), non-enzymatic chloramphenicol resistance gene (cmlA1g) and ADP-ribosylation genes (arr2, arr3). Outborn neonates had a significantly higher incidence of sepsis due to NDM-1 harboring isolates than their inborn counterparts. This study demonstrates the significance of both A. baumannii and other species of Acinetobacter in cases of neonatal sepsis over an extended period. Oxacillinases and blaNDM−1 are the major contributors to carbapenem resistance. The dissemination of the blaNDM−1 is likely linked to Tn125 in diverse clones of the isolates.

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Section: Discussionsupporting

confidence: 63%

Carbapenem Resistance in Acinetobacter baumannii and Other Acinetobacter spp. Causing Neonatal Sepsis: Focus on NDM-1 and Its Linkage to ISAba125

Chatterjee¹,

Datta²,

Roy³

et al. 2016

Front. Microbiol.

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“…Tigecycline is the first member of the glycylcyclines and is considered to be one of the few therapeutic options to treat multidrug-resistant Acinetobacter infections [5,6]. However, previous studies have shown that the tigecycline resistance rate of carbapenem-resistant A. nosocomialis was up to 21.5% in Taiwan [7].…”

Section: Introductionmentioning

confidence: 99%

Overexpression of AdeABC efflux pump associated with tigecycline resistance in clinical Acinetobacter nosocomialis isolates

Yang

Chen

Hsu

et al. 2019

Clinical Microbiology and Infection

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“…The growth of all four species of bacteria was inhibited by kanamycin at a concentration of 64 μg/mL. Recently, A. baumannii has become a significant opportunistic pathogen of nosocomial infection in Taiwan, especially in ICU [42]. However, the real challenge is the acquisition of MDR in hospital.…”

Section: Antibacterial Activities Of Tmcn-agnpsmentioning

confidence: 99%

Trimethylchitosan-Capped Silver Nanoparticles with Positive Surface Charge: Their Catalytic Activity and Antibacterial Spectrum Including Multidrug-Resistant Strains of <em>Acinetobacter baumannii</em>

Chang

Chen

Cheng

et al. 2017

Preprint

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Abstract:We report a facile route for the green synthesis of trimethylchitosan nitrate-capped silver nanoparticles (TMCN-AgNPs) with positive surface charge. In this synthesis, silver nitrate, glucose, and trimethyl chitosan nitrate (TMCN) were used as silver precursor, reducing agent, and stabilizer, respectively. The reaction was carried out in a stirred basic aqueous medium at room temperature without the use of energy-consuming or expensive equipment. We investigated the effects of the concentrations of NaOH, glucose, and TMCN on the particle size, zeta potential, and formation yield. The AgNPs were characterized by UV-visible spectroscopy, photon correlation spectroscopy, laser Doppler anemometry, transmission electron microscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. The catalytic activity of the TMCN-AgNPs was studied by the reduction of 4-nitrophenol using NaBH4 as a reducing agent. We evaluated the antibacterial effects of the TMCN-AgNPs on Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus using the broth microdilution method. The results showed that both gram-positive and gram-negative bacteria were killed by the TMCN-AgNPs at very low concentration (< 6.13 μg/mL). Moreover, the TMCN-AgNPs also showed high antibacterial activity against clinically isolated multidrug-resistant A. baumannii strains, and the minimum inhibitory concentration (MIC) was ≤ 12.25 μg/mL.

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Types and Prevalence of Carbapenem-Resistant Acinetobacter calcoaceticus-Acinetobacter baumannii Complex in Northern Taiwan

Cited by 19 publications

References 18 publications

Carbapenem Resistance in Acinetobacter baumannii and Other Acinetobacter spp. Causing Neonatal Sepsis: Focus on NDM-1 and Its Linkage to ISAba125

Carbapenem Resistance in Acinetobacter baumannii and Other Acinetobacter spp. Causing Neonatal Sepsis: Focus on NDM-1 and Its Linkage to ISAba125

Overexpression of AdeABC efflux pump associated with tigecycline resistance in clinical Acinetobacter nosocomialis isolates

Trimethylchitosan-Capped Silver Nanoparticles with Positive Surface Charge: Their Catalytic Activity and Antibacterial Spectrum Including Multidrug-Resistant Strains of <em>Acinetobacter baumannii</em>

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