Types of microorganisms in proximal caries lesion and ozone treatment

Abstract

The purpose of this clinical study was to investigate the types of microorganisms in deep proximal caries lesions and the efficacy of elimination of microorganisms after a 24 s ozone application to proximal cavity lesions prepared for restoration. Sixteen caries-active volunteers (female: 8, male: 8; age range: 35-55), with proximally situated deep caries lesions on premolars or molars, were included in the study. Each patient underwent two microbiological investigations. First, the caries decay before treatment was assessed. Second, the dentine tissue was assessed after the removal of necrotic tooth decay and 24 s ozone application. The prevalence of oral streptococci was determined. In addition, we isolated Candida albicans, Enterococcus faecalis and Peptostreptococcus spp. from deep proximal caries lesion in caries-active patients. All microorganisms found in the deep proximal caries lesions were destroyed following 24 s ozone application. Our findings suggest that S. anginosus group, C. albicans and E. faecalis should be considered as an index for caries activity in caries-active patients, but additional studies are necessary to confirm this suggestion. This study demonstrated that 24 s of gaseous ozone application to the deep proximal caries lesions effectively eliminated microbial species.

Isolation and Identification of Potentially Pathogenic Microorganisms Associated with Dental Caries in Human Teeth Biofilms

“…However, fewer studies investigated their cariogenic associations. Though S. salivarius and S. anginosus have been considered as oral commensal flora, some findings suggest that S. salivarius and S. anginosus isolated from deep proximal caries lesion could be considered as indices for caries activity [41,42]. Half a percent of sodium chloride and 2% of soy peptone were added into artificial saliva to simulate a real oral environment.…”

Direct Pulp Capping with Advanced Platelet-Rich Fibrin: A Report of Two Cases

“…Gaseous ozone stimulates dentinogenesis [ 22 ]. Our studies have proven that 24 s of gaseous ozone application removes cariogenic microbes in the cavity after removing infected dentin; it has a disinfection effect [ 13 ]. Ozone administration has been shown to improve metabolism in inflamed tissues, enhance tissue oxygenation, and reduce inflammation.…”

“…Under anesthesia, pulp wound hemostasis and cavity disinfection with ozone gas was performed for 24 s using an ozone generator (Prozone, TIP TOP TIPS Sarl, Rolle, Switzerland) [ 13 ]. Venous blood (10 mL) was obtained from the patient by venipuncture and centrifuged at 1300 rpm for 14 min (Duo Centrifuge/Process for PRF, Nice, France).…”

Minimally invasive treatment of dental caries – a clinical study

“…Our study is based on the treatment of dentine wounds with ozone. Our previous studies found that gaseous ozone in 24 seconds destroys all microorganisms in the prepared cavity in deep approximal lesions [16,17]. Other authors found that remineralization improves the hardness of enamel and dentin after gaseous ozone application [18,19].…”