Typing for HLA‐D Determinants. Comparison of Typing Results using Homozygous Stimulating Cells and Primed Cultures

Abstract

Typing for HLA‐D determinants, using primed lymphocyte typing (PLT), has been compared to the results obtained using conventional homozygous stimulating cell primary MLC tests. The HLA‐Dw1, ‐Dw2 and ‐Dw3 specificities were studied. Preliminary results indicate that these two methods essentially type for the HLA‐D determinants and that reproducible results can be obtained employing the PLT technique after 24 hours of incubation in the secondary cultures.

Specificity of Human Lymphocytes Primed against Allogeneic Cells in vitro. II. Discrimination and Cross‐Reactivity in Repeated Priming

“…Our findings seem to be in accordance with the concept of cross-reactivity in PLT and indicate that under the experimental conditions used, a second priming does not increase the discriminatory ability of the primed cells. That the specificity of allogeneic primed cells is not improved by specific restimulation is somewhat at variance with our previous findings (Hirschberg et al 1975), and might be explained by the use of different priming as well as restimulating procedures.…”

Detection of HLA‐D Clusters and Segregation Studies Using Primed LD Typing

“…One would assume that this discriminative ability is directed toward determinants in the HLA -D region. Evidence indicates that only HLA-D region antigens cause restimulation in PLT cells , Hirschberg et al 1976, Thomsen et al 1976). The existence of minor LD loci within the MHC and their restimulating capacity in PLT cannot be excluded.…”

“…Several studies now suggest that those antigens detected by the PLT test are the same as those detected by HTC testing , Hirschberg et al 1976, Thomsen et al 1976 the pattern of discrimination of each PLT over a panel of unrelated cells which had cell is required.…”

Summary of the First International Workshop on Human Primed LD Typing

“…The use of multiple combinations (PLT cells generated in a number of families) which identify a single cluster of individuds who share an HLA-D determinant is helpful in eliminating the non-HLA-D effects in PLT A number of other priming techniques, which take advantage of known HTC typing, have been used successfully t o generate PLT cells apparently specific for HLA-D determinants. Various combinations of homozygous typing cells (HTC) and HLA-D typed heterozygous cells have been used in stimulator-responder pairs attempting to generate cells primed against a single HLA-D difference (Hirschberg et al 1975, Thomsen et al 1976, Hartzman et al 1977, Jaramillo et al 1977, Reinsmoen e t al. 1977.…”

“…The unknown cells which give rise to such a secondary response, therefore, possess similar if not identical LD antigens to the priming (reference) cell. This procedure has led to a method for typing of surface antigens encoded by the HLA-D region (Hirschberg et al 1975, Sheehy et al 1975, Crosier e t al. 1977b).…”