Typing of human and bovine Staphylococcus aureus by RAPD-PCR and ribotyping-PCR

Abstract

Aims: To investigate genetic diversity among Staphylococcus aureus and to delineate the geographical distribution of the strains found. Methods and Results: RAPD‐PCR and ribotyping‐PCR were employed for the characterization of Staph. aureus isolates from bovine and nosocomial origin. Among the strains, five to nine groups were distinguished by RAPD‐PCR, depending on which primer was used, while ribotyping‐PCR distinguished seven ribotypes. Conclusions, and Significance and Impact of the Study: These results demonstrate the genetic heterogeneity of the strains studied, and the large dissemination of some clones throughout different regions and hosts, findings that may allow the monitoring of Staph. aureus infections.

Growth inhibition of Staphylococcus aureus by chicken egg yolk antibodies

“…The major reservoirs of the organism are infected udders, teat canals, and teat lesions, but these bacteria have also been found on teat skin, muzzles, nostrils, and vagina (Roberson et al 1994). The bacteria are spread to uninfected quarters by teat cup liners, milkers' hands, washcloths, and flies (Chambers 2001;Marshall et al 2000;Pereira et al 2002;Roberson et al 1994;Roghmann et al 2005). S. aureus produces toxins that destroy cell membranes and can directly damage milk-producing tissue (Balaban and Rasooly 2000;Bohach and Foster 2000).…”

The prevalence of virulent clonal strains of mutans streptococci in vivo and co-culture succession of the strains in vitro—Virulence potential of mutans streptococci

“…The protocol for DNA amplification was based on Pereira [21], with a little modification. For DNA extraction, cells were washed in 0.1 M Tris buffer, lysed with lysozyme + 10% SDS and extracted with phenol-chloroform.…”

“…Quality was estimated from A 260 /A 280 ratio. RAPD working primers [21] The amplification was done in a Perkin Elmer 9600 Thermal Cycler. PCR cycling procedures were as follows; 50 cycles of 94˚C for 1 min, 37˚C for 1 min and 72˚C for 1 min.…”

Infecções intramamárias causadas por Staphylococcus aureus e suas implicações em paúde pública

“…Com relação à identificação da origem do S. aureus no leite de consumo, diversos métodos bioquímicos e moleculares têm sido utilizados para diferenciar as cepas provenientes de infecções humanas e animais. A técnica de PCR associada à ribotipagem tem-se mostrado bastante útil para esta finalidade, devido à sensibilidade e rapidez, possibilitando o monitoramento de cepas e o estudo taxonômico molecular (PEREIRA et al, 2002).…”