Typing of staphylococcal cassette chromosome mec encoding methicillin resistance in Staphylococcus aureus isolates in Ahvaz, Iran

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen. We sought to determine the frequency of the different types of SCCmec in MRSA isolates by performing a cross-sectional study. A total of 72 S. aureus isolates were collected from Imam Khomeini and Golestan hospitals and analysed for MRSA and SCCmec typing by multiplex PCR. The pattern of antibiotic resistance among S. aureus isolates was determined by disc diffusion analysis. Of the 72 S. aureus isolates, 29 (40.27%) were recognized as MRSA. SCCmec type III was the most common type, with 55.17% (16/29), followed by type II with 27.58% (8/29); type IV with 10.34% (3/29); and type I with 6.89% (2/29). All 29 MRSA isolates were resistant to chloramphenicol and erythromycin. In addition, resistance to cephalothin, gentamicin, clindamycin, ciprofloxacin, tetracycline and rifampicin was seen in 24 (75%), 26 (63.4%), 17 (94.4%), 27 (71.05%), 10 (71.42%) and 13 (68.42%) MRSA isolates, respectively. A decreased sensitivity of MRSA to the antibiotics used was observed, with type III SCCmec being the predominant isolate.

Characterization of SCCmec, spa types and Multi Drug Resistant of meticillin-resistant Staphylococcus aureus isolates among inpatients and outpatients in a referral hospital in Shiraz, Iran.

“…In our study, vancomycin antibiotic was a highly effective antibiotic against MRSA isolates, which was consistence with other studies in Iran [11,20,22].…”

“…SCCmec typing provides useful information about the resistance of genes to methicillin and to identify the origin of strains [28,29]. In our study, SCCmec typeIII was the most common type, which was similar to other reports in Iran and other countries [11,18,22,26,27,30,31]. However, in a study by Havaie et al SCCmec type ІV was the most common SCCmec type [32].…”

Characterization of SCCmec, spa types and Multi Drug Resistant of meticillin-resistant Staphylococcus aureus isolates among inpatients and outpatients in a referral hospital in Shiraz, Iran.

“…SCCmec typing provides useful information about the resistance of genes to methicillin, and to identify the origin of strains [28,29]. In our study, SCCmec typeIII was the most common type, which was similar to other reports in Iran and other countries [11,18,22,26,27,30,31]. However, in a study by Havaie et al SCCmec type ІV was the most common SCCmec type [32].…”

<p>Characterization of <em>SCCmec</em>, <em>Spa</em> Types and Multidrug Resistant of Methicillin-Resistant <em>Staphylococcus aureus</em> Isolates in Ahvaz, Iran</p>

“…18 In Iran, the total number of MRSA infection cases has increased, thereby introducing a serious problem in the form of nosocomial infections. 16 A previous study on MRSA in Khuzestan province revealed a dramatic annual increase in the proportion of isolates resistant to methicillin during the recent years, rising from 60% in 2017 and 2018 to a peak of 77% in 2019. [19][20][21][22] Such differences in the prevalence of MRSA infections in our region might reflect the fact that in these areas, different policies are considered for infection control and other factors.…”

“…The presence of SCCmec genes in S. aureus isolates was checked using PCR amplification, as previously explained by Moosavian et al 16 Five MRSA strains, NCTC10442 (SCCmec I), NCTC N315 (SCCmec II), NCTC 85/2082 (SCCmec III), NCTC CA05 (SCCmec IVa), and JCSC3624 (SCCmec V) were used as a positive control and uninoculated medium as negative control.…”