Tyrosinase-based biosensor for determination of bisphenol A in a flow-batch system

Highly sensitive electrochemical biosensor for bisphenol A detection based on a diazonium-functionalized boron-doped diamond electrode modified with a multi-walled carbon nanotube-tyrosinase hybrid film

“…Up to date, many electrochemical biosensors based on enzymes (Dempsey et al, 2004;Wang et al, 2014;Pan et al, 2015;Portaccio et al, 2013;Kochana et al, 2015), aptamers (Kang et al, 2011;Zhu et al, 2015;Ragavan et al, 2013;Zhu et al, 2014), antibodies (Piao et al, 2008;Rahman et al, 2007;Wang et al, 2014) and peptides (Yang et al, 2014;Qu et al, 2013;Kim et al, 2015) have been fabricated for the detection of BPA. Among them, the enzyme tyrosinase was applied to the measurement of BPA.…”

Sensitive Voltammetric Amoxicillin Sensor Based on TiO2 Sol Modified by CMK‐3‐type Mesoporous Carbon and Gold Ganoparticles

“…The ability of the developed electrochemical method to determine AMX in mineral and environmental water samples and pharmaceutical product, without any pre‐treatment, was confirmed. The monosegmented sequential injection approach and the flow‐batch mode were employed for introducing solutions into the measuring cell and to the signal measurement, respectively.…”

Efficient Immobilization of Tyrosinase Enzyme on Layered Double Hydroxide Hybrid Nanomaterials for Electrochemical Detection of Polyphenols

“…In the reduction-based mode, the same estimation gives a sensitivity of 1.20 μA/ng.mL −1 equivalent to 498 A.M −1 or 3952 A.M −1 ·cm −2 . These values are high as compared to others previously reported for tyrosinase-based systems [16]- [18], [23], [24], [28], [63], [66] which is useful for the comfort of detection and its quantification.…”

“…However, it is possible to determine a mean molecular mass of polyphenols at 415 g.mol −1 , by taking into account the molar mass of each compound present in the mixture and its relative percentage determined from previous analyses [44]. Based on this estimation, we obtain a linear range of 0-2.4 μM, leading to the conclusion that it is particularly well-adapted to the detection of low concentrations (typical limits of detection and linear ranges usually range from nM to hundreds of μM in the case of tyrosinase-based biosensors [16]- [18], [20]- [25], [27], [28], [63], [65]. In the context of tea polyphenol detection, our maximal concentrations for a linear biosensor response are in agreement with those obtained in tea decoctions, which are around 2-5 μM [17].…”

“…Different approaches have been developed for the immobilization of tyrosinase: silica sol-gel composite films [18], tyrosinase-modified boron-doped diamond electrodes [19], [20], tyrosinase entrapped in an agarose-guar gum [21], glassy carbon electrode modified by gold nanoparticles [22], tyrosinase-FeO 3 nanoparticles-chitosan [23], use of polyaniline-carbon nanofibers or nanotubes [24]- [27], diazonium salt [17] or titania sol-gel matrix [28] … Among host matrixes already used for the immobilization of enzymes recognizing polyphenols [6]- [11], [18]- [30], Layered Double Hydroxide (LDH) nanomaterials are attractive candidates for electrochemical analysis in general and for enzyme immobilization [31], [32], since they exhibit many advantageous features in the development of hybrid biomaterials. LDHs are an unusual family of layered materials consisting of positively charged layers with charge balancing anions inserted between these layers.…”