Tyrosinase inhibitory activity of Garcinia daedalanthera Pierre

Abstract

The tyrosinase enzyme catalyzes the first stage in two reactions in the synthesis of melanin (the hydroxylation of l-tyrosinase becomes 3,4-dihydroxyphenylalanine (l- DOPA) and oxidation from L-dopa to dopaquinone). Hyperpigmentation in human skin is a general phenomenon that is not desirable. Researchers were encouraged to identify potential new tyrosinase inhibitors for cosmetics, especially anti-hyperpigmentation. This study purposed to determine the inhibitory activity of the tyrosinase enzyme from Garcinia daedalanthera Pierre leaves. The extract was obtained by maceration successively method. The anti-tyrosinase assay used the spectrophotometric method at 490 nm. The enzyme used tyrosinase from mushrooms lyophilized powder (Sigma), and the substrate used 3,4-dihydroxy-L-phenylalanine, L-DOPA (Sigma). The tyrosinase inhibitory assay results of 100 ppm (triplicate) showed the leaves extract, including ethyl acetate extract of 33.42 ± 5.98 %, hexane extract was 50.67 ± 0.47 %, and methanol extract of 50.68 ± 1.87 %, respectively. Moreover, the stem bark has activity as follows methanol extract of 43.76 ± 1.41 % and the ethyl acetate extract of 55.71 ± 2.80 %. Percentage inhibition of the positive control (kojic acid) was 65.07 ± 0.03 % at 100 ppm.

Aktivitas Anti-elastase dan Antioksidan dari Ekstrak Etanol Kayu Bangkal (Nauclea subdita) Korth. Steud. dengan Variasi Metode Ekstraksi

“…Alkaloid, flavonoid, dan polifenol merupakan zat aktif antioksidan. Jumlah komponen flavonoid dalam ekstrak mempengaruhi potensi aktivitas anti-elastase (Ambarwati et al, 2019). Jika dibandingkan dengan metode ekstraksi lainnya (perkolasi, maserasi, dan air suling), dan jenis pelarut (seperti etanol 96%, etanol 70%, dan etil asetat), sokletasi dengan etanol 96% memberikan konsentrasi fenolik total terbesar yaitu 81,121±1,66 mg EAG/g ekstrak (Rahmi et al, 2021).…”

“…Uji anti-elastase menggunakan porcine pancreatic elastase (PPE) dan substrat N-Succ-(Ala)-3-p-nitroanilide (SANA) (Ambarwati et al, 2019)…”

Tyrosinase Inhibitory Activity of Garcinia latissima Miq. Extracts

“…There are some plants from Garcinia family that have been tested for their tyrosinase. They are : G. daedalanthera Pierre, G. atroviridis Griff., G. xanthochymus, G. livingstonei, and G. kola [1][2][3][4] .…”