Tyrosinase inhibitory components from <i>Aloe vera</i> and their antiviral activity

Kim, Young Ho; Yoon, Ju-Yeon; Yang, Seo Young; Choi, Seung‐Kook; Kwon, Sun Jung; Cho, Insook; Jeong, Manhee; Choi, Gug-Seoun

doi:10.1080/14756366.2016.1235568

Cited by 39 publications

(33 citation statements)

References 18 publications

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“…Enzyme assay was performed according to the modified methods in the previous papers 7 . For the calculation of inhibitory activity, 130 μL of tyrosinase (about 46 units/mL) solvated in 0.1 mM phosphate buffer (pH: 6.8) and 20 μL of 1–0.0078 mM concentrations of the inhibitors were mixed in a 96-well plate, and then 50 μL of 2 mM L-tyrosine in buffer was added in mixture.…”

Section: Methodsmentioning

confidence: 99%

“…Tyrosinase (EC 1.14.18.1) is a multifunctional copper-containing enzyme that is important for melanin biosynthesis related to pigment production in the skin, the browning of vegetables and cuticle formation in insects 7 , 8 . Two coppers of this enzyme participate in the conversion of L-tyrosine to 3,4-dihydroxy-L-phenylalanine (L-DOPA) during the early stage of melanin biosynthesis, as well as the conversion of L-DOPA to dopaquinone, the precursor of melanin 8 , 9 .…”

Section: Introductionmentioning

confidence: 99%

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Kushenol A and 8-prenylkaempferol, tyrosinase inhibitors, derived from Sophora flavescens

Kim

Cho

et al. 2018

Journal of Enzyme Inhibition and Medicinal Chemistry

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Tyrosinase is known for an enzyme that plays a key role in producing the initial precursor of melanin biosynthesis. Inhibition of the catalytic reaction of this enzyme led to some advantage such as skin-whitening and anti-insect agents. To find a natural compound with inhibitory activity towards tyrosinase, the five flavonoids of kushenol A (1), 8-prenylkaempferol (2), kushenol C (3), formononetin (4) and 8-prenylnaringenin (5) were isolated by column chromatography from a 95% methanol extract of Sophora flavescens. The ability of these flavonoids to block the conversion of L-tyrosine to L-DOPA by tyrosinase was tested in vitro. Compounds 1 and 2 exhibited potent inhibitory activity, with IC50 values less than 10 µM. Furthermore, enzyme kinetics and molecular docking analysis revealed the formation of a binary encounter complex between compounds 1–4 and the enzyme. Also, all of the isolated compounds (1–5) were confirmed to possess antioxidant activity.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Kushenol A and 8-prenylkaempferol, tyrosinase inhibitors, derived from Sophora flavescens

Kim

Cho

et al. 2018

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…Hence, the use of tyrosinase inhibitors as food preservatives, especially from natural sources, is therefore considered as an alternative approach not only to prevent the enzymatic browning of plant-derived foods but also in insect pest control [41]. The highest tyrosinase inhibition observed after treatment with the extracts from aerial organs of the studied species can be related with their higher content in phenolic compounds, since it is known that such molecules can significantly inhibit this enzyme [43].…”

Section: Enzyme Inhibitory Activitiesmentioning

confidence: 94%

“…Quinones have an unpleasant flavour and color and react with proteins, reducing its digestibility and the bioavailability of essential amino acids, with consequent nutritional and economical losses during food storage [41,42]. Tyrosinase is also involved in melanogenesis and cuticle formation in insects [43]. Hence, the use of tyrosinase inhibitors as food preservatives, especially from natural sources, is therefore considered as an alternative approach not only to prevent the enzymatic browning of plant-derived foods but also in insect pest control [41].…”

Section: Enzyme Inhibitory Activitiesmentioning

confidence: 99%

Phenolic Profile, Toxicity, Enzyme Inhibition, In Silico Studies, and Antioxidant Properties of Cakile maritima Scop. (Brassicaceae) from Southern Portugal

Placines

Castañeda-Loaiza

Rodrigues

et al. 2020

Plants

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Cakile maritima Scop. (sea rocket) is an edible halophyte plant with several ethnomedicinal uses. This work reports the chemical profile and bioactivities of food grade extracts from sea rocket organs. Toxicity was determined on mammalian cells, and phenolic profiling and the quantitation of the main metabolites were made by high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS). Enzymatic inhibition was determined towards acetyl- and butyrylcholinesterase (AChE, BuChE), α-glucosidase, α-amylase, and tyrosinase. Docking studies were performed to tyrosinase, on the major metabolites, and samples were tested for antioxidant properties. Extracts were not toxic, were constituted mainly by flavonoids, and some compounds (roseoside and oleuropein) are here described for the first time in the species. The aerial organs’ ethanol extract had relevant activity towards 2,2-diphenyl-1-picrylhydrazyl [DPPH, half maximal inhibitory concentration (IC50) = 0.59 mg/mL], and ferric-reducing activity power (FRAP, IC50 = 0.99 mg/mL). All samples were more active towards AChE than on BuChE. The ethanol fruits’ extract inhibited α-glucosidase [2.19 mmol of equivalent of acarbose (ACAE)/g]. Samples were active against tyrosinase, especially the aerial organs’ ethanol extracts [25.9 mg of equivalent of kojic acid (KAE)/g]. Quercetin and kaempferol glycosides fit well into the enzymatic pocket of tyrosinase. Our results suggest sea rocket as a candidate to be further explored as a source of bioactive products.

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“…[108,181] Aloin and aloe emodin in A. vera show antiplsomodial potential. [182] and A. pulcherrima leaves showed antiplasmodial activities [183] while its latex antimalarial activity [184] is used by Luhya Community of Kakamega East sub-County, Kenya; [185 ] A new compound, 9-dihydroxyl-2'-O-(Z)-cinnamoyl-7methoxy-aloesin (1), and eight known compounds (2-9) were isolated from Aloe vera tyrosinase inhibitory activity in Pepper mild mottle virus. [186] The extracts show better antimicrobial activity against bacterial strains as compared to fungal strains.…”

Section: Antidiabetesmentioning

confidence: 99%

Untitled

Upadhyay¹

2018

IJGP

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The present review explains therapeutic and pharmaceutical potential of Aloe vera a well-known plant grows in semi-arid climate of tropical and subtropical regions. This article emphasizes important uses of A. vera constituents as dietary nutraceutical, medicinal, and therapeutic potential. Plant is cultivated for agricultural and medicinal and decoration purposes for indoors as a potted plant. Plant is a good depository of chemical constituents which display a very wide array of biological activities such as anticancer, antiparasitic, antidiabetic, anti-inflammatory, anti-arthritic, antiparasitic, antitumor, antioxidant, chemopreventive, hepatoprotective, and gastroprotective. Plant is used to prepare skin protective/care gels mainly for soothing, moisturizing, and wound healing. Thick watery plant sap works are added as key ingredient in many beauty products. Plant leaves are used to generate aroma, beverages, skin lotion, cosmetics, or ointments for minor burns. Plant contains vitamins, enzymes, minerals, sugars, lignin, saponins, salicylic acids, and amino acids as main ingredients. Plant is a good source of Vitamins A, C, and E, which are antioxidants. It also contains Vitamin B12, folic acid, and choline watery juicy of A. vera leaf which contains important minerals such as calcium, chromium, copper, selenium, magnesium, manganese, and potassium. Plant ingredients were found active against gingivitis, psoriasis, and used for herbal therapy in inflammatory bowel disease. A. vera contains important fatty acids mainly steroids such as cholesterol, campesterol, β-sitosterol, and lupeol. Aloin and emodin act as analgesics, antibacterials, and antivirals while lupeol shows antiseptic and analgesic properties. It also contains auxins and gibberellin hormones that help in wound healing and have anti-inflammatory action. Saponins that are the soapy substances display cleansing and antiseptic properties.

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Tyrosinase inhibitory components from Aloe vera and their antiviral activity

Cited by 39 publications

References 18 publications

Kushenol A and 8-prenylkaempferol, tyrosinase inhibitors, derived from Sophora flavescens

Kushenol A and 8-prenylkaempferol, tyrosinase inhibitors, derived from Sophora flavescens

Phenolic Profile, Toxicity, Enzyme Inhibition, In Silico Studies, and Antioxidant Properties of Cakile maritima Scop. (Brassicaceae) from Southern Portugal

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