Tyrosine Phosphorylation of the Rho Guanine Nucleotide Exchange Factor Trio Regulates Netrin-1/DCC-Mediated Cortical Axon Outgrowth

DeGeer, Jonathan; Boudeau, Jérôme; Schmidt, Susanne; Bedford, Fiona K.; Lamarche-Vane, Nathalie; Debant, Anne

doi:10.1128/mcb.01264-12

Cited by 63 publications

(70 citation statements)

References 55 publications

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“…Trio is ubiquitously expressed, but the role of Trio in platelet function has not been established. In vitro, Trio has been shown to be phosphorylated by the Src kinase Fyn (42). Our study has shown that RhoG activation is regulated by SFK, and the role of Fyn in the regulation of GPVImediated platelet activation is well known.…”

Section: Discussionmentioning

confidence: 83%

RhoG Protein Regulates Glycoprotein VI-Fc Receptor γ-Chain Complex-mediated Platelet Activation and Thrombus Formation

Kim

Dangelmaier

Bhavanasi

et al. 2013

Journal of Biological Chemistry

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Background: RhoG is a ubiquitously expressed member of the Rho family of GTPases. Results: RhoG-deficient platelets display severely impaired GPVI-dependent platelet activation. Conclusion: RhoG plays an important role in GPVI-FcR␥ complex-mediated platelet activation and thrombus formation. Significance: Our study enhances the understanding of the molecular mechanisms of GPVI-FcR␥ complex activation.

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Section: Discussionmentioning

confidence: 83%

RhoG Protein Regulates Glycoprotein VI-Fc Receptor γ-Chain Complex-mediated Platelet Activation and Thrombus Formation

Kim

Dangelmaier

Bhavanasi

et al. 2013

Journal of Biological Chemistry

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“…Analogously, in mammalian tumor cells, Abl phosphorylates the C-terminal portion of Trio to stimulate its Rho GEF activity, and this promotes metastasis (Sonoshita et al, 2015). The domain of Trio that becomes phosphorylated by Abl in the mouse tumor model, however, and by Fyn in mouse neurons (DeGeer et al, 2013) does not exist in Drosophila Trio. Moreover, previous experiments have shown that the Rho GEF domain of Trio is dispensable for axon and dendrite development in Drosophila and C. elegans, and that it is the Rac GEF activity of Trio that controls neuronal morphogenesis (Iyer et al, 2012;Shivalkar and Giniger, 2012;Song and Giniger, 2011;Steven et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

The Abl pathway bifurcates to balance Enabled and Rac signaling in axon patterning in Drosophila

et al. 2017

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The Abl tyrosine kinase signaling network controls cell migration, epithelial organization, axon patterning and other aspects of development. Although individual components are known, the relationships among them remain unresolved. We now use FRET measurements of pathway activity, analysis of protein localization and genetic epistasis to dissect the structure of this network in Drosophila. We find that the adaptor protein Disabled stimulates Abl kinase activity. Abl suppresses the actin-regulatory factor Enabled, and we find that Abl also acts through the GEF Trio to stimulate the signaling activity of Rac GTPase: Abl gates the activity of the spectrin repeats of Trio, allowing them to relieve intramolecular repression of Trio GEF activity by the Trio N-terminal domain. Finally, we show that a key target of Abl signaling in axons is the WAVE complex that promotes the formation of branched actin networks. Thus, we show that Abl constitutes a bifurcating network, suppressing Ena activity in parallel with stimulation of WAVE. We suggest that the balancing of linear and branched actin networks by Abl is likely to be central to its regulation of axon patterning.

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“…6G,H), Trio could be activated through the intracellular domain of Drl receptors, including the tyrosine kinase domain. Interestingly, recent reports indicate that Trio is phosphorylated at multiple tyrosine residues and that the tyrosine phosphorylations appear to be essential for its Rho GEF activity (DeGeer et al 2013;Sonoshita et al 2014). It is thus of interest to test whether Trio could be phosphorylated in response to Wnt5 signals in v'ada neurons.…”

Section: Discussionmentioning

confidence: 99%

Adult Drosophila sensory neurons specify dendritic territories independently of dendritic contacts through the Wnt5–Drl signaling pathway

et al. 2015

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Sensory neurons with common functions are often nonrandomly arranged and form dendritic territories in stereotypic spatial patterns throughout the nervous system, yet molecular mechanisms of how neurons specify dendritic territories remain largely unknown. In Drosophila larvae, dendrites of class IV sensory (C4da) neurons completely but nonredundantly cover the whole epidermis, and the boundaries of these tiled dendritic fields are specified through repulsive interactions between homotypic dendrites. Here we report that, unlike the larval C4da neurons, adult C4da neurons rely on both dendritic repulsive interactions and external positional cues to delimit the boundaries of their dendritic fields. We identify Wnt5 derived from sternites, the ventral-most part of the adult abdominal epidermis, as the critical determinant for the ventral boundaries. Further genetic data indicate that Wnt5 promotes dendrite termination on the periphery of sternites through the Ryk receptor family kinase Derailed (Drl) and the Rho GTPase guanine nucleotide exchange factor Trio in C4da neurons. Our findings thus uncover the dendritic contact-independent mechanism that is required for dendritic boundary specification and suggest that combinatory actions of the dendritic contact-dependent and -independent mechanisms may ensure appropriate dendritic territories of a given neuron.

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Tyrosine Phosphorylation of the Rho Guanine Nucleotide Exchange Factor Trio Regulates Netrin-1/DCC-Mediated Cortical Axon Outgrowth

Cited by 63 publications

References 55 publications

RhoG Protein Regulates Glycoprotein VI-Fc Receptor γ-Chain Complex-mediated Platelet Activation and Thrombus Formation

RhoG Protein Regulates Glycoprotein VI-Fc Receptor γ-Chain Complex-mediated Platelet Activation and Thrombus Formation

The Abl pathway bifurcates to balance Enabled and Rac signaling in axon patterning in Drosophila

Adult Drosophila sensory neurons specify dendritic territories independently of dendritic contacts through the Wnt5–Drl signaling pathway

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