Über den Einfluß von Magnesium- und Calciumchlorid auf einige Fermentwirkungen

Keeser, E.

doi:10.1007/bf01863261

Cited by 2 publications

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“…Thus Keeser (1931) found that calcium chloride inhibited the digestion of gelatine by trypsin, and Grob (1946) the action of trypsin on casein.…”

Section: Infectivity Of Trypsin-treated Virusmentioning

confidence: 99%

TRYPTIC INACTIVATION OF THE RECEPTOR‐DESTROYING ENZYME OF V. CHOLERAE AND OF THE ENZYMIC ACTIVITY OF INFLUENZA VIRUS

Stone

1949

Aust J Exp Biol Med

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In earlier work (Burnet and Stone, 1947) on the technique of preparation of the receptor-destroying enzyme (RDE) of V. ckolerae it was noticed that in some of the cultures on soft agar plates there was a sudden fall in the concentration of the enzyme if incubation was extended beyond 16 to 18 hours. The effect did not occur regularly and appeared to he correlated with the development of high alkalinity in the cultures. It was originally observed with our standard 4Z strain, but could not be demonstrated when this strain was re-examined later. Several other strains of V. ckolerae regularly showed disappearance of R-DE together with a strongly alkaline reaction when incuhated for 24 to 48 hours.A second enzyme of the cholera vibrio also under investigation in this laboratory, the mucinase acting on glandular mucin (Burnet, 1948a), was not destroyed under these conditions. The phenomenon was studied therefore with a view to obtaining a technique for the preparation of mucinase free from RDE. It was found that the vibrio cultures contained a factor, probably an enzyme resembling trypsin, which destroyed RDE under conditions of alkalinity and absence of calcium ions. Destruction could also he brought about with minute amounts of trypsin acting under similar conditions. Furthermore, when influenza virus was exposed to the action of trypsin its enzymic activity was reduced as tested hy its capacity to destroy both the virus receptors on the redcell and the inhibitory activity of miicoid. MATERIALS AND METHODS.Agar extracts of V. cholerae. These were prepared aa previously described (Burnet and Stone, 1947). Briefly, ] p.c. agar plates heavily inoculated with V. cholerae were incubated for 16 hours at 37° C. or for longer periods where specified. The agar was then removed into

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“…Thus Keeser (1931) found that calcium chloride inhibited the digestion of gelatine by trypsin, and Grob (1946) the action of trypsin on casein.…”

Section: Infectivity Of Trypsin-treated Virusmentioning

confidence: 99%