Über die Bildung von Melanin in Muskelzellen nach der direkten Übertragung von RNA aus HARDING-PASSEY-Melanomzellen

Abstract

Zusammenfassung: RNA aus HARDING-PASSEY-Melanomzellen wird mit Hilfe von Mikroglaskanülen in Muskelzellen injiziert und der Einbau von [ 3 H]L-DOPA als Vorstufe des Melanins autoradiographisch untersucht. Ein Einbau von L-DOPA in den Muskelzellen konnte nur nach der Injektion von Melanomzellen-RNA erreicht werden. RNA aus Rattenleberzellen und Ribonuclease-behandelte Melanomzellen-RNA bewirken nach Injektion in Summary: The formation of melanin in muscle cells after the direct transfer of RNA from Harding-Passey melanoma cells. RNA from HARDING-PASSEY melanoma cells was injected into muscle cells by means of micro glass cannulae and the incorporation of the labelled melanin precursor [ 3 H]L-DOPA was studied autoradiographically. Incorporation of L-DOPA in muscle cells could be observed only after injection of RNA from melanoma cells. RNA from rat liver cells and ribonuclease-treated RNAVerschiedene Arbeitsgruppen berichteten in den letzten Jahren über die Aufnahme von Nucleinsäuren aus dem Kulturmedium durch Säugetierzellen 1 " 4 . Nur in wenigen Fällen konnte nachgewiesen werden, daß die aufgenommene Desoxyribonucleinsäure oder Ribonucleinsäure einen Einfluß

Simian virus 40 small-t protein is required for loss of actin cable networks in rat cells

“…We have employed a technique involving injection of macromolecules into single rat embryo fibroblast cells (REF) in culture to study the role of the SV40 large-T and small-t proteins in the dispersal of the actin cable networks (11,12). We find that injection of Hirt supernatant SV40 DNA or restriction endonuclease-generated or -cloned SV40 DNA fragments containing the sequences encoding an intact small-t protein, but only a substantially deleted large-T, induces the abortive disappearance of actin cables in recipient cells.…”

Molecular Cytogenetics

“…(1973a). Injections were made with glass microneedles using a Leitz micromanipulator according to the technique developed for mammalian cells by Graessmann (1970) and Graessmann and Graessmann (1971). Recipient glands were extirpated from the larvae and 10-10 ml of a RNA solution containing 2-3 mg/ml was injected into each of the 10-15 cells in one side of the proximal extremity of the salivary gland.…”