UCN-01 (7-hydroxystaurosporine) induces apoptosis and G1 arrest of both primary and metastatic oral cancer cell lines in vitro

Effects of high-dose major components in oral disinfectants on the cell cycle and apoptosis in primary human gingival fibroblasts in vitro

“…We analyzed a total of 1×10 4 cells for each sample and determined the percentages of cells in the G0/G1, S, and G2/M phases of the cell cycle using standard ModiFit and Cell Quest software (Becton Dickinson). The cellular DNA content was determined by flow cytometry, as described previously 23) . mM, 5.0×10 -1 mM for PVD-I) of each disinfectant, HGFs were harvested using trypsin-EDTA, washed with DMEM, resuspended in 100 μL of binding buffer supplemented with 5 μL of annexin V-FITC and 5 μL of PI solution, and treated in the dark for 15 min at room temperature.…”

The Regulatory Role of A Disintegrin and Metalloproteinase 28 on the Biologic Property of Human Periodontal Ligament Stem Cells

“…To detect the proliferative activity of HPDLSCs, the cellular DNA content from each stage of five groups was determined by flow cytometry, as previously described. 17 We collected the attached cells using trypsin-EDTA and resuspended them in DMEM (without FBS). The cells were fixed for 30 minutes in an icecold 70% ethanol solution containing ribonuclease (2 mg/ml).…”

Influence of chlorine dioxide on cell death and cell cycle of human gingival fibroblasts

“…21 We induced the cells to re-enter the cell cycle by incubation in fresh medium with the disinfectants at the final concentration (determined from the growth assay results) for a further 24 h. The cellular DNA content was determined by flow cytometry, as described previously. 22 We collected the floating and attached cells using trypsin-EDTA and resuspended them in DMEM (no FBS). The cells were fixed for 30 min in an ice-cold 70% ethanol solution containing ribonuclease (RNase; 2 mg/ ml).…”