Influence of chlorine dioxide on cell death and cell cycle of human gingival fibroblasts

Abstract

These results suggest that ClO2 is sufficient for use as a dental disinfectant compared with H2O2 or NaOCl.

Comparison of Organic Tissue Dissolution Capacities of Sodium Hypochlorite and Chlorine Dioxide

“…Chlorine dioxide was selected for this study because of its strong antimicrobial properties, compatibility with living tissue, ease of use, and widespread availability (11,19). A recent study by Nishikiori et al (20) showed that ClO 2 and NaOCl arrested cells at the G0/G1 phase of the cell cycle; however, they did not induce apoptosis. Chlorinating agents, such as Cl 2 and NaOCl, used as disinfectants for drinking water react with natural organic matter to produce halogenated disinfectant by-products.…”

“…Its powerful oxidizing properties enable it to kill bacteria by disrupting the transport of nutrients across the cell wall (18). ClO 2 has recently come under consideration as a possible root canal irrigant because of its reported antibacterial activity and compatibility with living tissue (11,19,20). In addition, the recent detection of cytomegalovirus and Epstein-Barr virus associated with periradicular lesions (21) might promote the use of ClO 2 , which kills both enveloped and nonenveloped viruses (22).…”

Effect of chlorine dioxide and sodium hypochlorite on the dissolution of human pulp tissue – An in vitro study

“…All samples were weighed by a single investigator who was unaware of how each was to be treated. 11,12 The percentage of tissue weight loss in each specimen was calculated and was implemented to analyze statistically. The percentage of tissue weight loss after subjecting to the solutions was calculated Fig.…”

Defining the pathogenicity of creatine deficiency syndrome

“…Cells were grown to a density of 80% and the culture medium replaced by MEM with 0.5% FCS for synchronisation over 48 h. The cells were then transferred back to MEM +10% FCS for 24 h followed by harvesting and counting. 1.5 x 10 6 cells were fixed in 70% ethanol for at least 18 h at -20ºC, and treated with PI/RNase Staining Buffer (BD Pharmingen, San Diego, CA, USA) for 30 min at 37ºC in the dark (Kues, et al, 2000;Nishikiori, et al, 2008). The DNA content was analysed by flow cytometry using a BD FACSCanto II flow cytometer (BSBioscience, San Jose, CA).…”