UDP‐galactose: N‐acetylgalactosaminyl‐(N‐acetylneuraminyl) galactosyl‐glucosyl‐ceramide transferase activity in adult frog brain

Yiamouyiannis, J. A.; Dain, Joel A.

doi:10.1007/bf02530944

Cited by 20 publications

(2 citation statements)

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“…Lower species might be expected to reflect in some way a more primitive stage of ganglioside evolution (24). In this study, using siaiic acid as an index, no obvious phylogenetic trend was observed.…”

Section: Discussioncontrasting

confidence: 58%

A comparative study of gangliosides from the brains of various species

Schengrund

Garrigan

1969

Lipids

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A comparison is made of the sialic acid and hexose content of crude gangliosides from the brains of 13 species, including mammals, a bird, and amphibian, a reptile and fish. The sialic acid content is relatively constant from species to species. Gangliosides are less abundant in tissues other than the brain. Four species (cat, dog, pig and sheep) were selected for the determination of their major ganglioside subfractions. The ganglioside subfractions were isolated (after extraction and partition dialysis) using descending thin layer chromatography. The population of ganglioside molecules varied from species to species. From dog and sheep a mono‐, a di‐ and a trisialoganglioside were obtained; from cat a mono‐, a di‐ and two trisialogangliosides; and from pig a mono‐ and two disialogangliosides. Each ganglioside subfraction was found to contain glucose, galactose and galactosamine in the ratio of approximately 1∶2∶1. The fatty acid moieties consisted of more than 80% stearic acid with lesser amounts of arachidic, palmitic and behenic acid. Sphingosine analyses indicated ratios of sphingosine to icosisphingosine of 7∶3 for the monosialo‐, 1∶1 for the disialo‐ and 3∶7 for the trisialogangliosides.

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Section: Discussioncontrasting

confidence: 58%

A comparative study of gangliosides from the brains of various species

Schengrund

Garrigan

1969

Lipids

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“…Kanfer et al (12) have documented reaction f using rat kidney preparations, and their evidence also suggested the occurrence of reaction g. Basu et al (16). have reported the presence of an enzyme which catalyzes reaction g in chicken embryo brain, and Yiamouyiannis and Dain (39) have found a similar enzyme in frog brain. It is pertinent to note that brain tissue from patients afflicted with infantile amaurotic familial idiocy accumulate galNAc-gal-glc-cer (40) as well as galNAc-(neuNAc)gal-glc-cer (Tay-Sachs' ganglioside).…”

Section: Identification Of the Reaction Productmentioning

confidence: 96%

Biosynthesis of glycolipids: Incorporation of N‐acetyl galactosamine by a rat brain particulate preparation

Handa

Burton

1969

Lipids

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A mixture of UDP‐(N‐acetyl)glucosamine‐114C and UDP‐(N‐acetyl)galactosamine‐114C (7∶3) served as a substrate to demonstrate in young rat brain tissue the presence of enzymes which catalyzes the reactions: UDP‐glcNAc⇌UDP‐galNAc (E.C.5.1.3) and UDP‐galNAc+ gal‐glc‐cer→ galNAc‐gal‐glc‐cer+UDP. The glycolipid acceptor specificity was examined and preliminary kinetic data were obtained for the UDP‐(N‐acetyl)galactosamine: gal‐glc‐ceramide N‐acetyl galactosamine transferase. The products of the reaction were identified. The relationship of this reaction to ganglioside biosynthesis is discussed.

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