UDP-Glucuronyltransferase Kinetics for 3-Trifluoromethyl-4-nitrophenol (TFM) in Fish

Kane, Andrew S.; Kahng, Myong Won; Reimschuessel, Renate; Nhamburo, Patson T.; Lipsky, Michael

doi:10.1577/1548-8659(1994)123<0217:ugkftn>2.3.co;2

Cited by 42 publications

(29 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TFM has similar effects on oxidative ATP production in rainbow trout mitochondria (Birceanu et al 2011), suggesting that any TFM-induced perturbations such as reduced internal ATP, PCr, and glycogen concentrations and lactacidosis, would be quickly reversed in nontarget fishes as well. Given that most fishes have greater capacity to clear TFM from their bodies (Dawson et al 2002;Vue et al 2002) than do sea lampreys (Kane et al 1994), it is likely that any physiological impairment arising from incidental exposure to TFM would be minimal, but this possibility needs to be confirmed by additional laboratory and field-based studies.…”

Section: Conclusion and Implications For Sea Lamprey Controlmentioning

confidence: 99%

Recovery of Larval Sea Lampreys from Short‐Term Exposure to the Pesticide 3‐Trifluoromethyl‐4‐Nitrophenol: Implications for Sea Lamprey Control in the Great Lakes

et al. 2012

View full text Add to dashboard Cite

For over 50 years, invasive sea lamprey Petromyzon marinus populations have been controlled in the Great Lakes using the pesticide 3-trifluoromethyl-4-nitrophenol (TFM), which is applied to streams containing larval sea lampreys. The specificity of TFM is due to the sea lamprey's relative inability to detoxify it using glucuronidation; this inability causes death by interfering with the production of ATP, the main energy currency of cells. TFM treatments typically last 12 h, but given the sea lamprey's relative inability to detoxify TFM we predicted that TFM-induced homeostatic disturbances and toxicity would occur following shorter periods of exposure. Accordingly, we exposed larval sea lampreys to the 12-h LC99.9 (i.e., the concentration lethal to 99.9% of the test subjects over 12 h) of TFM for 4 or 6 h and monitored the survival and replenishment of energy stores and metabolite concentrations in surviving sea lampreys over a 24-h depuration period. Minimal mortality (∼20%) was observed during the post-TFM recovery, despite respective 50% and 70% reductions in brain and liver ATP, 70-90% decreases in brain phosphocreatine (PCr), and 30-50% reductions in brain and liver glycogen with corresponding increases in lactate. While relatively resistant to TFM, muscle still underwent a 55% decline in PCr. However, energy stores and metabolites returned to preexposure levels 4-12 h after TFM exposure. We conclude that sea lampreys can rapidly restore homeostasis following shorter-term TFM exposure. The resilience of sea lampreys to shorter-term TFM exposure suggests that there is no merit in reducing TFM treatment times from 12 h, which could lead to "treatment residuals" that survive lampricide treatments. This capacity to reverse TFM-induced perturbations also suggests that larvae seen emerging from the stream substrate late in a treatment, or that find refuge near streambanks or in isolated pools, could be another source of treatment residuals, leading to increased sea lamprey parasitism of sport and commercial fisheries in the Great Lakes.

show abstract

Section: Conclusion and Implications For Sea Lamprey Controlmentioning

confidence: 99%

Recovery of Larval Sea Lampreys from Short‐Term Exposure to the Pesticide 3‐Trifluoromethyl‐4‐Nitrophenol: Implications for Sea Lamprey Control in the Great Lakes

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Cortisol has been shown to induce liver glycogenolysis in fish [ 37 ], and, therefore, the elevation of cortisol levels post-TFM exposure may be required to facilitate the supply of G6P substrate for glucuronic acid production and TFM elimination in the hours immediately following exposure. Most non-target fishes, rainbow trout in particular, do have a high capacity to detoxify TFM [ 11 , 16 ] and would rely on glucuronidation to eliminate the lampricide from the body [ 12 , 43 ]. Moreover, TFM and TFM-glucuronide have been detected in the muscle fillet of fish up to 12 h following a routine TFM application [ 16 ], suggesting that detoxification still occurs for several hours following exposure to the lampricide.…”

Section: Discussionmentioning

confidence: 99%

Post-exposure effects of the piscicide 3-trifluoromethyl-4-nitrophenol (TFM) on the stress response and liver metabolic capacity in rainbow trout (Oncorhynchus mykiss)

Birceanu

Wilkie

2018

PLoS ONE

View full text Add to dashboard Cite

The piscicide 3-trifluoromethyl-4-nitrophenol (TFM) has been used to control invasive sea lamprey (Petromyzon marinus) populations in the Great Lakes for almost 60 years. Applied to rivers and streams containing larval lampreys, TFM seldom harms non-target fishes, but the effects of sub-lethal treatments on fish physiology are not well understood. We examined the effects of 9 h exposure to TFM on the stress axis and liver metabolic capacity of rainbow trout (Oncorhynchus mykiss) using in vivo and in vitro approaches. The fish that had been acutely exposed to TFM in vivo had increased plasma cortisol levels at 12 h post-treatment, but TFM exposure did not interfere with in vitro cortisol production in head kidney preparations. Subjecting trout to an acute handling stressor 12 h post-TFM exposure resulted in a relative attenuation of the plasma cortisol and glucose response compared to pre-stress levels. We conclude that routine TFM treatments can lead to elevations of plasma cortisol following exposure, plus a relative dampening of the stress response in rainbow trout, with high cortisol levels lasting at least 12 h post-treatment. Since the ability of the fish to produce cortisol and the liver metabolic capacity were not compromised following TFM exposure, it is likely that their ability to cope with other stressors is not altered in the long-term.

show abstract

“…are conjugated with glucuronic acid via UDPGTs (George, 1994). Among the drugs and pesticides that are conjugated via UDPGTs are chloramphenicol (Cravedi et al, 1985), organophosphates (Takimoto et al, 1987), and phenols (Lech, 1973;Kane et al, 1994). Aflatoxicol B1 glucuronide is also formed in rainbow trout exposed to this hepatocarcinogen (Loveland et al, 1984).…”

Section: Udp-glucuronosyltransferase (Udpgt) Kineticsmentioning

confidence: 99%

“…In the past, differences in UDPGT-based conjugation have helped to understand distinct sensitivities among fish species to pollutants or drugs. Kane et al (1994) found that the lower V max /K m ratio in sea lamprey (Petromyzon marinus) as compared to channel catfish, rainbow trout and bluegill helped to explain a high sensitivity to 3-trifluoromethyl-4-nitrophenol (TFM), due to lower UDPGT-based conjugation.…”

Section: Udp-glucuronosyltransferase (Udpgt) Kineticsmentioning

confidence: 99%

Kinetics of hepatic phase I and II biotransformation reactions in eight finfish species

González

Reimschuessel

Shaikh

et al. 2009

Marine Environmental Research

Self Cite

View full text Add to dashboard Cite

Hepatic microsomes and cytosols of channel catfish (Ictalurus punctatus), rainbow trout (Oncorhynchus mykiss), Atlantic salmon (Salmo salar), red tilapia (Oreochromis sp.), largemouth bass (Micropterussalmoides), striped bass (Morone saxatilis), hybrid striped bass (M. saxatilis x M. crysops), and bluegill (Lepomis macrochuris) (n=8) were used to study the kinetics of phase I (ECOD, EROD, PROD, BROD) and phase II (UDP-glucuronosyltransferase (UDPGT)-, sulfotransferase (ST)- and glutathione-s-transferase (GST)-mediated) reactions. The best catalytic efficiency for ECOD and GST activities was performed by channel catfish, Atlantic salmon, rainbow trout and tilapia. The highest EROD catalytic efficiency was for Atlantic salmon. None of the species had either PROD or BROD activities. Rainbow trout had very similar UDPGT catalytic efficiency to tilapia, channel catfish, Atlantic salmon, largemouth bass and bluegill. Sulfotransferase conjugation had no significant differences among the species. In summary, tilapia, channel catfish, Atlantic salmon and rainbow trout had the best biotransforming capabilities; striped bass, hybrid striped bass and bluegill were low metabolizers and largemouth bass shared some capabilities with both groups.

show abstract

UDP-Glucuronyltransferase Kinetics for 3-Trifluoromethyl-4-nitrophenol (TFM) in Fish

Cited by 42 publications

References 21 publications

Recovery of Larval Sea Lampreys from Short‐Term Exposure to the Pesticide 3‐Trifluoromethyl‐4‐Nitrophenol: Implications for Sea Lamprey Control in the Great Lakes

Recovery of Larval Sea Lampreys from Short‐Term Exposure to the Pesticide 3‐Trifluoromethyl‐4‐Nitrophenol: Implications for Sea Lamprey Control in the Great Lakes

Post-exposure effects of the piscicide 3-trifluoromethyl-4-nitrophenol (TFM) on the stress response and liver metabolic capacity in rainbow trout (Oncorhynchus mykiss)

Kinetics of hepatic phase I and II biotransformation reactions in eight finfish species

Contact Info

Product

Resources

About