UeLa Cell-Adherent Escherichia coli in Children with Diarrhea in Thailand

Chatkaeomorakot, Arunsri; Echeverria, Peter; Taylor, David N.; Bettelheim, K. A.; Blacklow, Neil R.; Sethabutr, Orntipa; Seriwatana, Jitvimol; Kaper, James B.

doi:10.1093/infdis/156.4.669

Cited by 46 publications

(41 citation statements)

References 12 publications

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“…A DNA probe composed of a 1-kDa portion of the EAF plasmid was developed by isolating plasmid fragments associated with adhesion (10,144). This probe has been used to detect EPEC in various epidemiological investigations (56,65,80,82,106,128,144), and hybridization with the probe correlates well with the production of LA (10,33,36,56,65,71,128,146). In two EPEC isolates, the EAF probe hybridized with chromosomal DNA, indicating a chromosomal location for this sequence in these isolates (148).…”

Section: Epec Adherencementioning

confidence: 99%

Adhesion and its role in the virulence of enteropathogenic Escherichia coli

Law

1994

Clin Microbiol Rev

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Enteropathogenic Escherichia coli (EPEC) organisms are an important cause of diarrheal disease in young children. The virulence of EPEC is a multifactorial process and involves a number of distinct stages. Initial adherence to intestinal mucosa is mediated by fimbriae which bring about a distinct form of adhesion, localized adhesion. Intimate adhesion of the bacterium to the eukaryotic membrane occurs, resulting in the activation of signal transduction pathways. Microvilli are disrupted and effaced from the apical membrane which then cups around the organism to form pedestal structures, the attaching and effacing lesion. Diarrhea may be produced by alteration of the permeability of the apical membrane and also through a malabsorption mechanism. The pathways involved in the production of the attaching and effacing lesion are described. EPEC organisms were originally thought to belong to a number of distinct serogroups; it is now apparent that many isolates belonging to these serogroups are not pathogenic or belong to other pathogenic groups of E. coli. In addition, isolates falling outside of these serogroups are considered to be true EPEC. The definition of EPEC based on serotyping is inaccurate and should be replaced by methods that specifically detect the virulence properties of EPEC.

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Section: Epec Adherencementioning

confidence: 99%

Adhesion and its role in the virulence of enteropathogenic Escherichia coli

Law

1994

Clin Microbiol Rev

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“…EPEC strains also cause attaching and effacing lesions on eukaryotic cells that involve a 94-kDa protein encoded by the chromosomal eae gene (25,28). The pathogenicity of EPEC strains has been demonstrated in human volunteers, and their role in childhood diarrhea was confirmed in epidemiological studies (9,10,11,15,18,26). Atypical EPEC strains do not carry the EAF plasmid, were found to exhibit LAL, and have been isolated from acute infantile diarrhea in São Paulo (35).…”

mentioning

confidence: 91%

Comparison of DNA Hybridization and PCR Assays for Detection of Putative Pathogenic Enteroadherent Escherichia coli

Scaletsky¹,

Fabbricotti²,

Aranda³

et al. 2002

J Clin Microbiol

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The correlation of the different adherence patterns with DNA probes and PCR primers for the identification of Escherichia coli was analyzed in isolates from children, less than 2 years of age with or without diarrhea, from different regions of Brazil. A total of 1,428 isolates obtained from 338 patients and 322 control children were studied. The enteropathogenic E. coli (EPEC) adherence factor (EAF) probe was shown to be as good as the HEp-2 adhesion assay for the detection of typical EPEC strains. The DNA probes used to detect diffusely adhering E. coli and enteroaggregative E. coli (EAEC) showed low sensitivities (64 and 50%, respectively), and the best method of identifying these organisms in clinical research remains the HEp-2 adherence assay. The "bundle-forming pilus" (BFP) and the EAEC PCR assays could be used instead of the DNA probes as a screening method for typical EPEC and EAEC carrying the EAEC probe sequence in the clinical laboratory. In our study, only typical EPEC strains that carried EAF and BFP were associated with acute diarrhea. The LA exhibited by typical enteropathogenic E. coli (typical EPEC) is mediated by an inducible bundle-forming pilus (BFP), whose expression correlates with the presence of a plasmid designated the EPEC adherence factor (EAF) plasmid (1, 17). EPEC strains also cause attaching and effacing lesions on eukaryotic cells that involve a 94-kDa protein encoded by the chromosomal eae gene (25, 28). The pathogenicity of EPEC strains has been demonstrated in human volunteers, and their role in childhood diarrhea was confirmed in epidemiological studies (9,10,11,15,18,26). Atypical EPEC strains do not carry the EAF plasmid, were found to exhibit LAL, and have been isolated from acute infantile diarrhea in São Paulo (35).The adherence of many enteroaggregative E. coli strains requires the presence of a plasmid that contains genes encoding the AA (38). Epidemiological studies have implicated EAEC as a cause of diarrhea in children in developing countries, and the pathogenic potential of EAEC in human infections was substantiated by challenge studies (5,10,23,27,39).Two factors, F1845 and AIDA-I were found to encode DA in diffusely adhering E. coli (DAEC) (4, 6). Several recent studies have implicated DAEC strains as agents of diarrhea, while other studies have not recovered DAEC strains more frequently from diarrheal patients than from asymptomatic controls (2,15,16,21,24).DNA probes derived from the adherence-related sequences have been constructed (3,4,6,17,25,30) and used in hybridization assays for the detection of the different putative categories of diarrheagenic E. coli in many epidemiologial studies. PCR primers have been also developed for several of the categories of diarrheagenic E. coli (8,14,20,36).In order to optimize screening methods for putative pathogenic enteroadherent E. coli in the clinical laboratory we analyzed the correlation of the different HEp-2 adherence patterns with DNA probes and PCR primers in E. coli isolates from different urban centers of Brazil...

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“…One of the most striking clinical features of EPEC infections is the propensity of these enteropathogenic strains to cause disease in infants (6,10,19), with few reports of cases of EPEC associated with diarrhea in older children and adults (52). Infants are more likely to develop diarrhea during the first episode of colonization with EPEC than they are during subsequent encounters (11).…”

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confidence: 99%

Antigen Detection in Enteropathogenic Escherichia coli Using Secretory Immunoglobulin A Antibodies Isolated from Human Breast Milk

et al. 2000

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Enteropathogenic Escherichia coli (EPEC) produces a characteristic attaching and effacing (A/E) lesion in the small intestines of infected children. The immune response to EPEC infection remains poorly characterized. The molecular targets that elicit protective immunity against EPEC disease are unknown. In this study protein antigens from EPEC were identified using secretory immunoglobulin A (sIgA) antibodies isolated from milk from Mexican women by Western blot analysis. Purified sIgA antibodies, which inhibit the adherence of EPEC to cells, reacted to many EPEC proteins, the most prominent of which were intimin (a 94-kDa outer membrane protein) and two unknown proteins with apparent molecular masses of 80 and 70 kDa. A culture supernatant protein of 110 kDa also reacted strongly with the sIgA antibodies. The molecular size of this protein and its reactivity with specific anti-EspC antiserum suggest that it is EPEC-secreted protein C (EspC).

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UeLa Cell-Adherent Escherichia coli in Children with Diarrhea in Thailand

Cited by 46 publications

References 12 publications

Adhesion and its role in the virulence of enteropathogenic Escherichia coli

Adhesion and its role in the virulence of enteropathogenic Escherichia coli

Comparison of DNA Hybridization and PCR Assays for Detection of Putative Pathogenic Enteroadherent Escherichia coli

Antigen Detection in Enteropathogenic Escherichia coli Using Secretory Immunoglobulin A Antibodies Isolated from Human Breast Milk

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