UGT2B13 and UGT2C1 are involved in lambda-cyhalothrin resistance in Rhopalosiphum padi

Wang, Suji; Liu, Xi; Tang, Hongcheng; Li, Mengtian; Gao, Peng; Peng, Xiong; Chen, Maohua

doi:10.1016/j.pestbp.2023.105528

Cited by 5 publications

(7 citation statements)

References 48 publications

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“…The expression of RpCSP7 is significantly induced in the aphid after exposure to lambda-cyhalothrin. Previous studies showed that increasing expression of detoxifying enzymes and target-site VGSC mutation is related to pyrethroid resistance in this aphid. ,− In this study, we provided evidence that RpCSP7 was involved in the high resistance of R. padi to lambda-cyhalothrin.…”

Section: Discussionsupporting

confidence: 54%

“…padi to bifenthrin and deltamethrin. We speculated that the cross-resistance of the LC-RR strain to deltamethrin and bifenthrin is likely due to other resistance mechanisms, such as target-site insensitivity and increased metabolism through detoxifying enzymes. ,− …”

Section: Discussionmentioning

confidence: 99%

“…The procedure of the qRT-PCR was as follows: 30 s at 95 °C, with 45 cycles of 10 s at 95 °C, followed by 30 s at 58 and 72 °C for 30 s. According to previous publications, the elongation factor 1α (EF-1 α ) and β-actin (β-actin) genes can be well-used as internal references in the gene expression analyses of R. padi. , These two genes were adopted as internal references in this study. There were three technical replicates and three biological replicates for each treatment.…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies have identified some mechanisms associated with high levels of resistance to bifenthrin and lambda-cyhalothrin in R. padi, including a target-site VGSC (voltage-gated sodium channel) M918L mutation and overexpression of cytochrome P450 monooxygenases (P450) genes or uridine diphosphate (UDP)-glycosyltransferase (UGTs) genes. ,− Additionally, a carboxylesterase (CarE) has been found to be involved in the resistance of R. padi to cyhalothrin .…”

Section: Introductionmentioning

confidence: 99%

“…padi strain (LC-RR) exhibited an extremely high resistance to lambda-cyhalothrin. Complicated resistance mechanisms, including voltage-gated sodium channel mutation and increased expression level of detoxifying enzymes, were found to contribute to the resistance. ,− However, the resistance level of the LC-RR strain was increased under the selection of lambda-cyhalothrin, indicating that some novel mechanisms may be involved in the resistance.…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of the Chemosensory Protein CSP7 Gene Contributed to Lambda-Cyhalothrin Resistance in the Bird Cherry-Oat Aphid Rhopalosiphum padi

Gao,

Tan,

et al. 2023

J. Agric. Food Chem.

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Lambda-cyhalothrin is one of the most important pyrethroids used for controlling wheat aphids. Extensive spraying of lambda-cyhalothrin has led to the development of high resistance to this pyrethroid inRhopalosiphum padi. The mechanisms of resistance are complex and not fully understood. In this study, we found that a laboratory-selected strain of R. padi showed extremely high resistance to lambda-cyhalothrin and cross-resistance to bifenthrin and deltamethrin. The expression level of RpCSP7 was significantly elevated in the resistant strain compared to that in the susceptible strain. Knockdown of RpCSP7 increased the susceptibility of R. padi to lambda-cyhalothrin, whereas the susceptibility to bifenthrin and deltamethrin was not significantly changed. The recombinant RpCSP7 displayed a high affinity for lambda-cyhalothrin but no affinities to bifenthrin and deltamethrin. These findings suggest that the overexpression of RpCSP7 contributes to the resistance of R. padi to lambda-cyhalothrin. This study provides valuable insights into CSP-mediated insecticide resistance in insects.

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