UGT86C11 is a novel plant UDP-glycosyltransferase involved in labdane diterpene biosynthesis

Abstract

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Identification and functional characterization of novel plant UDP-glycosyltransferase (LbUGT72B10) for the bioremediation of 3,4-dichloroaniline

“…This result conforms to the published values for other UGTs, with their optimal temperature range between 30-40 °C. 47–50…”

“…This result conforms to the published values for other UGTs, with their optimal temperature range between 30-40 °C. [47][48][49][50] We further investigated the thermostability of LbUGT72B10 (Fig. 5C).…”

Whole transcriptome analysis identifies full-length genes for neoandrographolide biosynthesis from Andrographis alata, an alternate source for antiviral compounds

“…Sun et al (2019)functionally characterized recombinant UGT73AU1, UGT71, UGT76, UGT85, and UGT88 from A. paniculata and reported that ApUGT37AUI could convert andrograpanin to neoandrographolide [37]. However, an extensive functional characterization of 23 UGTs from A. paniculata revealed that UGT86CII could be the most probable enzyme, and it showed relatively higher catalytic activity than UGT73AUI ( Srivastava et al, 2021 ). A. alata transcriptome contained 103 transcripts coding for 21 UGTs, including a full-length gene for UGT73AUI (AaUGT73AUI) and a partial gene for UGT86CII (AaUGT86CII).…”

“…The Andrographis genus contains 29 species, including A. paniculata, A. alata, A. gracilis, A. affinis, A. longipedunculata , A. elongata , and A. macrobotrys ( Gnanasekaran and Murthy, 2015 ). Genes coding for NADPH: cytochrome P450 reductase, ent-copalyl diphosphate synthase, geranylgeranyl pyrophosphate synthases (GGPPS), and UDP-glucotransferase (UGT) were cloned from A. paniculata and functionally characterized ( Lin et al, 2017 , Misra et al, 2015 , Wang et al, 2019 , Srivastava et al, 2021 ). Chromosome-level genome sequence and transcriptome data are also available for A. paniculata ( Liang et al, 2020 ).…”

Bridging fungal resistance and plant growth through constitutive overexpression ofThchit42gene inPelargonium graveolens

“…ThActin was selected as the reference gene for normalizing the expression of the target gene. Relative transcript expression was determined using the 2 -ΔΔC T method (Srivastava et al, 2021). Triplicate reactions were conducted for each cDNA sample to ensure data correctness.…”