UHF-Dielectrophoresis Signatures as a Relevant Discriminant Electromagnetic Biomarker of Colorectal Cancer Stem Cells

Lambert, Élisa; Barthout, Elodie; Manczak, Rémi; Sadaa, Sofiane; Bessette, Barbara; Mathonnet, Muriel; Lalloué, Fabrice; Dalmay, Claire; Pothier, Arnaud

doi:10.1109/ims37962.2022.9865336

Cited by 1 publication

(3 citation statements)

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“…Consequently, the crossover frequency fx02 depends mainly on the dielectric properties of the cell's content. In this way, we have previously demonstrated that the UHF-DEP signature appears as a relevant biomarker for CSCs discrimination and detection among a heterogeneous cancer cell population [10], [11], [15], [16]. Hence, biological cells can be individually electro-manipulated by the motion induced by the DEP force.…”

Section: A Working Principle Of the Dep Cytometermentioning

confidence: 98%

“…Apostream, a commercial system, already exploits low DEP frequency regime to isolate circulating cancer cells (CTCs) from for patient liquid biopsies (blood) [7]- [9]. However, in the frame of solid cancer investigations, it has been established that UHF-DEP might be more discriminant toward CSCs and can be relevant to use in order to isolate such cells from a solid tumor [10], [11]. The originality of this paper especially relies on the used frequency range to sort cells: a high frequency regime (between 300MHz and 3GHz).…”

Section: Introductionmentioning

confidence: 99%

“…To do so and to demonstrate the feasibility of the proposed approach, we will exploit the specificities of the dielectrophoretic signatures previously obtained in [10], reminded in Fig. 1a, where a colorectal cancer cell line SW620 has been cultured in two different conditions: one with a large majority of differentiated cancer cells (Normal Medium culture condition) and another enriching the cell population in CSCs (Define Medium culture condition).…”

Section: Introductionmentioning

confidence: 99%

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Lab-On-Chip UHF-Dielectrophoretic Cytometer for Colorectal Cancer Stem Cells Sorting

Lambert

Barthout

Provent

et al. 2023

IEEE J. Electromagn. RF Microw. Med. Biol.

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In this paper, we introduce a method to exploit ultra-high dielectrophoresis (UHF-DEP) with a microfluidic radio frequency device able to sort biological cells by deflecting the trajectory of the targeted population in a continuous flow. This study highlights the capability of a high frequency lab-on-chip implemented UHF-DEP cytometer to isolate cancer stem cells (CSCs). Actually, CSCs sorting by an efficient, fast and low-cost method remains an issue. Above 20 MHz, the intracellular dielectric properties of cells determine the dielectrophoretic behavior of cells and is then very sensitive to the cell cytoplasm content. The proposed cell sorting principle exploits the combination of the fluidic drag and the repulsive dielectrophoretic forces in order to isolate CSCs hidden within differentiated cancer cells. We previously demonstrated the UHF-DEP behavior of cells, as a new type of electromagnetic biomarkers to discriminate cancer stem cells among a tumor population from colorectal cancer cell line.

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