UHPLC-QTOFMS-based metabolomic analysis of serum and urine in rats treated with musalais containing varying ethyl carbamate content

Han, ZhanJiang; Guo, Dongqi; Xiang, Yanju

doi:10.1007/s00216-020-02900-5

Cited by 5 publications

(6 citation statements)

References 48 publications

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“…Even so, we cannot draw conclusions that no differences were observed in the metabolic profiles of serum among the groups. Supervised OPLS-DA can reveal the specific variables that caused differences among groups relative to unsupervised PCA [ 28 ]. Therefore, the multivariate statistical analysis using OPLS-DA was further performed to detect serum-specific metabolites that differ between the CON and HMB treatment, and the scatter plots are presented in Figure 2 B.…”

Section: Resultsmentioning

confidence: 99%

Alterations of the Muscular Fatty Acid Composition and Serum Metabolome in Bama Xiang Mini-Pigs Exposed to Dietary Beta-Hydroxy Beta-Methyl Butyrate

Zheng

Song

Guo

et al. 2021

Animals

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This study aimed to investigate the effects of dietary beta-hydroxy beta-methyl butyrate (HMB) supplementation on muscular lipid metabolism in Bama Xiang mini-pigs. Thirty-two piglets (8.58 ± 0.40 kg, barrow) were selected and fed a basal diet supplemented either with 0 (control), 0.13%, 0.64%, or 1.28% HMB for 60 days. Throughout the experiments, they had free access to clean drinking water and diets. Data of this study were analyzed by one-way ANOVA using the SAS 8.2 software package, followed by a Tukey’s studentized range test to explore treatment effects. The results showed that compared to the control, 0.13% HMB decreased the intramuscular fat (IMF) content and increased polyunsaturated fatty acids (PUFAs) in Longissimus thoracis muscle (LTM), and increased the n3 PUFAs in soleus muscles (SM, p < 0.05). Moreover, HMB supplementation led to alterations in the mRNA expression of genes related to lipid metabolism. Serum metabolome profiling showed that in both LTM and SM of Bama Xiang mini-pigs, N-Methyl-l-glutamate was positively correlated with SFA and nummularine A was negatively correlated with C18:3n3 PUFA (p < 0.05). Therefore, N-Methyl-l-glutamate and nummularine A might be potential biomarkers of the HMB-supplemented group. These results suggested that dietary HMB supplementation could decrease the IMF content and increase n3 PUFAs as well as regulate the related metabolites (N-Methyl-l-glutamate and nummularine A) in the serum of pigs.

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Section: Resultsmentioning

confidence: 99%

Alterations of the Muscular Fatty Acid Composition and Serum Metabolome in Bama Xiang Mini-Pigs Exposed to Dietary Beta-Hydroxy Beta-Methyl Butyrate

Zheng

Song

Guo

et al. 2021

Animals

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“…As shown in Figure S1A,B , the shape of the peak is good, and the distribution is relatively uniform, suggesting the stability and reliability of the metabolomics profiles. Unlike unsupervised PCA, supervised OPLS-DA has the ability to detect the specific variables that lead to differences among groups [ 19 ]. Considering the reduced L* value and increased a* value in pigs offered low-protein diets (the −20% group vs. the control group), we performed OPLS-DA to explore the subtle differences in metabolic profiles among the two groups.…”

Section: Resultsmentioning

confidence: 99%

“…The role of protein-restriction diets in saving protein resources and decreasing the emission of nitrogen in urea and feces has been well characterized. However, most of the studies related to protein restriction have focused on the short-term effects on non-Chinese commercial breeds [ 19 ]. Moreover, our understanding of the long-term effects of high-protein and low-protein diets on pig production performance (growth performance, carcass characteristics, and meat quality) is incomplete for native Chinese pig breeds.…”

Section: Discussionmentioning

confidence: 99%

Effects of Long-Term Protein Restriction on Meat Quality and Muscle Metabolites of Shaziling Pigs

Zheng

Duan

et al. 2022

Animals

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Background: It has been demonstrated that low-protein diets can improve the meat quality of pork. This study aimed to investigate the effects of long-term protein restriction from piglets to finishing pigs for 24 weeks on meat quality and muscle metabolites of Shaziling pigs. Results: Compared to the control group, reducing dietary protein levels by 20% reduced the L* value (p < 0.05), increased the a* value (p < 0.01), and tended to decrease pressing loss (p = 0.06) of longissimus thoracis muscle (LTM). Furthermore, compared to the control group, the −20% group had significantly lower levels of muscular danazol, N,N-dimethyl-Safingol, and cer(d18:0/14:0) (p < 0.05), all of which were positively associated with the L* value and negatively associated with the a* value (p < 0.05). Therefore, danazol, N,N-dimethyl-Safingol, and cer(d18:0/14:0) might be potential biomarkers for meat color. Conclusions: These results indicated that reducing dietary crude protein by 20% for 24 weeks could improve meat quality and alter muscular metabolites of Shaziling pigs, and the improvement in meat quality might be ascribable to decreased danazol, N,N-dimethyl-Safingol and cer(d18:0/14:0).

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“…In addition to reducing biological effects, irradiation at doses of 1.0 or 3 kGy also improved the safety, aroma, and flavor of alcoholic beverages (Křížek et al., 2012). The improved safety of alcoholic beverages was associated with a reduction in putrescine, cadaverine, tyramine, and EC, with various BA levels below 10 mg/kg and EC levels reduced to 22.895 ppm (Křížek et al., 2012; Wang et al., 2020).…”

Section: Prevention Of Nmh Contamination In the Process Of Alcoholic ...mentioning

confidence: 99%

Mitigation of microbial nitrogen‐derived metabolic hazards as a driver for safer alcoholic beverage choices: An evidence‐based review and future perspectives

Liao,

Asif,

Huang

et al. 2023

Comp Rev Food Sci Food Safe

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Alcoholic beverages have been enjoyed worldwide as hedonistic commodities for thousands of years. The unique quality and flavor are attributed to the rich microbiota and nutritional materials involved in fermentation. However, the metabolism of these microbiota can also introduce toxic compounds into foods. Nitrogen‐derived metabolic hazards (NMH) are toxic metabolic hazards produced by microorganisms metabolizing nitrogen sources that can contaminate alcoholic beverages during fermentation and processing. NMH contamination poses a risk to dietary safety and human health without effective preventive strategies. Existing literature has primarily focused on investigating the causes of NMH formation, detection methods, and abatement techniques for NMH in fermentation end‐products. Devising effective process regulation strategies represents a major challenge for the alcoholic beverage industry considering our current lack of understanding regarding the processes whereby NMH are generated, real‐time and online detection, and the high degradation rate after NMH formation. This review summarizes the types and mechanisms of nitrogenous hazard contamination, the potential risk points, and the analytical techniques to detect NMH contamination. We discussed the changing patterns of NMH contamination and effective strategies to prevent contamination at different stages in the production of alcoholic beverages. Moreover, we also discussed the advanced technologies and methods to control NMH contamination in alcoholic beverages based on intelligent monitoring, synthetic ecology, and computational assistance. Overall, this review highlights the risks of NMH contamination during alcoholic beverage production and proposes promising strategies that could be adopted to eliminate the risk of NMH contamination.

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UHPLC-QTOFMS-based metabolomic analysis of serum and urine in rats treated with musalais containing varying ethyl carbamate content

Cited by 5 publications

References 48 publications

Alterations of the Muscular Fatty Acid Composition and Serum Metabolome in Bama Xiang Mini-Pigs Exposed to Dietary Beta-Hydroxy Beta-Methyl Butyrate

Alterations of the Muscular Fatty Acid Composition and Serum Metabolome in Bama Xiang Mini-Pigs Exposed to Dietary Beta-Hydroxy Beta-Methyl Butyrate

Effects of Long-Term Protein Restriction on Meat Quality and Muscle Metabolites of Shaziling Pigs

Mitigation of microbial nitrogen‐derived metabolic hazards as a driver for safer alcoholic beverage choices: An evidence‐based review and future perspectives

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