UHPLC-Triple-TOF-MS Characterization, Antioxidant, Antimicrobial and Antiproliferative Activity of Raspberry (Rubus idaeus L.) Seed Extracts

Marić, Boško; Abramović, Biljana F.; Ilić, Nebojša; Bodroža‐Solarov, Marija; Pavlić, Branimir; Oczkowski, Michał; Wilczak, Jacek; Četojević-Simin, Dragana; Šarić, Ljubiša; Teslić, Nikola

doi:10.3390/foods12010161

Cited by 8 publications

(9 citation statements)

References 48 publications

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“…Extract with citric acid/betaine/water with molar ratio 2:1:2 exhibited the highest concentration of EA (3.20 mg/100 g extract), while extracts with tartaric acid/betaine and malic acid/betaine (both with molar ratio 2:1) showed the content of 3.18 mg/100 g extract and 3.01 mg/100 g extract, respectively. Also, Marić and coauthors prepared extracts by using an ultrasound-assisted extraction technique, and found a free EA content from 43.05 mg/100 g to 46.76 mg/100 g extracts depending of raspberry variety [19]. The content of free ellagic acid in our extracts is comparable with results from both publications.…”

Section: Determination Of Free and Total Ellagic Acid Contentsupporting

confidence: 86%

“…Bioactive phytochemicals from berries' seed oil processing by-products were reported for its chemical richness, heterogeneity, biological, and functional properties [18]. To date, the polyphenolic profile of defatted raspberry seeds was investigated, mainly to determine the bioactivity potential of extracts [19,20]. Marić and coauthors showed that defatted raspberry seeds extract possess high antioxidative, and high antiproliferative activities but low antimicrobial activity, and recommended that they be used in food, cosmetic, and pharmaceutical industries [19].…”

Section: Introductionmentioning

confidence: 99%

“…To date, the polyphenolic profile of defatted raspberry seeds was investigated, mainly to determine the bioactivity potential of extracts [19,20]. Marić and coauthors showed that defatted raspberry seeds extract possess high antioxidative, and high antiproliferative activities but low antimicrobial activity, and recommended that they be used in food, cosmetic, and pharmaceutical industries [19]. In addition, in a study conducted by Wójciak and coworkers, it was shown that defatted strawberry seeds are a rich reservoir of phenolic compounds with significant antioxidant properties (tiliroside, kaempferol glucoside, and ellagic acid), and may serve as a valuable material for obtaining a beneficial additive for skin care products [16].…”

Section: Introductionmentioning

confidence: 99%

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Valorisation of Raspberry Seeds in Cosmetic Industry-Green Solutions

Ćirić,

Dabić Zagorac,

Sredojević

et al. 2024

Pharmaceutics

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The fruit processing industry generates large quantities of by-products well known to be rich in bioactive compounds with numerous nutritional properties and beneficial effects for human health. We developed a strategy to valorise raspberry seeds and obtain valuable ingredients with potential application in cosmetic skincare formulas. Cold press extraction technology was applied to extract oil, and the remaining defatted raspberry seed cake was treated with three proline based deep eutectic solvents (DES) to extract polyphenols. The most potent was proline/citric acid extract, with free and total ellagic acid content (52.4 mg/L and 86.4 mg/L), total phenolic content (TPC, 550.1 mg GAE/L) and radical scavenging activity (RSA, 4742.7 mmol TE/L). After the direct mixing of the extract and after encapsulation with starch as a carrier, the skincare emulsion and microemulsion were characterised by irritation potential (Zein test), transepidermal water loss (TEWL), red blood cell (RBC), and DPPH antioxidant test. The resulting preparations were of improved quality in comparison to the control hand cream, with a low skin irritation effect, lower TEWL, and higher antioxidant potential. This work complies with circular economy principles and green technology standards, and represents the efficient model on how to reuse natural resources through waste minimization.

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Section: Determination Of Free and Total Ellagic Acid Contentsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Valorisation of Raspberry Seeds in Cosmetic Industry-Green Solutions

Ćirić,

Dabić Zagorac,

Sredojević

et al. 2024

Pharmaceutics

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“…The total content of phenolic compounds determined with the Folin-Ciocalteau method confirms that raspberry fruits are a good source of antioxidant secondary metabolites [46,50,51]. The methods used, i.e., the ferric reducing antioxi-dant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical, and OH • scavenging assays, confirm the high free radical scavenging activity of secondary metabolites from Rubi idaei fructus raw material [28,29,47,[50][51][52][53][54][55]. This indicates that raspberry fruits can be used as a valuable nutritional component in the adjuvant therapy of oxidative stress-related diseases.…”

Section: Oxidative Stress and Its Effects And Defense Mechanisms Agai...mentioning

confidence: 76%

Nutritionally Important Pro-Health Active Ingredients and Antioxidant Properties of Fruits and Fruit Juice of Selected Biennial Fruiting Rubus idaeus L. Cultivars

Chwil,

Matraszek-Gawron,

Kostryco

et al. 2023

Pharmaceuticals

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Raspberry fruits are an important source of many biologically active chemical compounds exerting nutritional and pro-health effects. The study presents a comparative analysis of nutritionally important bioactive chemical compounds—polyphenols; flavonoids, including anthocyanins; vitamin C; amino acids; fatty acids; and primary metabolites—contained in the fruits of three biennial fruiting cultivars, R. idaeus ‘Glen Ample’, ‘Laszka’, and ‘Radziejowa’, i.e., common cultivars in Poland and Europe. The antioxidant activity of fresh fruits and juice was determined with five methods. The analyses revealed the strong free radical scavenging potential of the fruits and juice, confirmed by the high concentration of nutrients, e.g., polyphenols, anthocyanins, vitamin C, amino acids, and fatty acids. The antioxidant activity of the juice determined with the ferric reducing antioxidant power (FRAP) and OH radical methods was from 2.5 to 4.0 times higher than that of the fruits. The following orders of total polyphenol contents were established in the analyzed cultivars: ‘Glen Ample’ < ‘Laszka’ < ‘Radziejowa’ in the fruits and ‘Glen Ample’ < ‘Radziejowa’ < ‘Laszka’ in the juice. The highest antioxidant activity was exhibited by the ‘Radziejowa’ fruits. Given their high content of dietary fiber, the fruits of the analyzed raspberry cultivars can be consumed by dieting subjects. The concentrations of vitamin C (28–34 mg/100 g) and anthocyanins (20–34 mg/100 g) indicate the biological and pharmacological activity of these fruits. The main unsaturated fatty acids in the fruits were gamma-linoleic acid (C18:2n6c) and alpha-linolenic acid (C18:3n3), which neutralize excess free radicals. The amino acids nutritionally essential to humans were dominated by leucine, arginine, and phenylalanine. This is the first comparative analysis of the antioxidant activity of fruits and juice and the contents of selected active compounds in the fruits of biennial fruiting cultivars of R. idaeus, i.e., a highly commercialized crop in Europe.

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“…Acetic acid at pH 3 promoted a better recovery of phenolic compounds of jabuticaba peels than formic acid [ 16 ], used by Quatrin et al [ 3 ] and Plaza et al [ 17 ]. Ellagic acid contents were also higher than those determined for defatted raspberry seed extracts (43.05 to 46.76 mg/100 g) obtained by ultrasound-assisted extraction [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

Polyphenols in Jabuticaba (Plinia spp.) Peel Flours: Extraction and Comparative Evaluation of FTIR and HPLC for Quantification of Individual Compounds

Resende

Oliveira

Franca

2023

Foods

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Jabuticabas are wild fruits native to Brazil, and their peels, the main residue from jabuticaba processing, contain significant amounts of bioactive compounds, which are mostly phenolics. Conventional methods based on the estimation of total extractable phenolics (TEP—Folin–Ciocalteau) or total monomeric anthocyanins (TMA) have limitations and may not reflect the actual antioxidant potential of these peels. Analytical methods, such as high-performance liquid chromatography (HPLC), are more appropriate for the quantification of specific phenolics, and can be used as a reference for the construction of mathematical models in order to predict the amount of compounds using simple spectroscopic analysis, such as Fourier Transform Infrared Spectroscopy (FTIR). Therefore, the objectives of this study were (i) to evaluate the composition of specific polyphenols in flours prepared from jabuticaba peels and verify their correlation with TEP and TMA results from a previous study, and (ii) to employ FTIR coupled with chemometrics to predict the concentrations of these polyphenols in jabuticaba peel flours (JPFs) using HPLC as a reference method. Cyanidin-3-glucoside (C3G), ellagic acid (EA) and delphinidin-3-glucoside (D3G) were the main polyphenols found in the samples. The C3G contents ranged from 352.33 mg/100 g (S10) to 1008.73 mg/100 g (S22), with a strong correlation to TMA (r = 0.97; p = 0.00) and a moderate correlation to TEP (r = 0.45; p = 0.02). EA contents ranged from 163.65 mg/100 g (S23) to 334.69 mg/100 g (S11), with a moderate to strong correlation to TEP (r = 0.69; p = 0.00). The D3G values ranged from 94.99 mg/100 g (S10) to 203.36 mg/100 g (S5), with strong correlations to TMA (r = 0.91; p = 0.00) and C3G levels (r = 0.92; p = 0.00). The developed partial least squares-PLS models based on FTIR data provided satisfactory predictions of C3G and EA levels, reasonably matching those of HPLC.

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UHPLC-Triple-TOF-MS Characterization, Antioxidant, Antimicrobial and Antiproliferative Activity of Raspberry (Rubus idaeus L.) Seed Extracts

Cited by 8 publications

References 48 publications

Valorisation of Raspberry Seeds in Cosmetic Industry-Green Solutions

Valorisation of Raspberry Seeds in Cosmetic Industry-Green Solutions

Nutritionally Important Pro-Health Active Ingredients and Antioxidant Properties of Fruits and Fruit Juice of Selected Biennial Fruiting Rubus idaeus L. Cultivars

Polyphenols in Jabuticaba (Plinia spp.) Peel Flours: Extraction and Comparative Evaluation of FTIR and HPLC for Quantification of Individual Compounds

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