UJI AKTIVITAS ANTIBAKTERI EKSTRAK METANOL DAUN BAWANG BATAK (Allium chinense G. Don) TERHADAP Streptococcus mutans DAN Bacillus cereus SEBAGAI BAKTERI GRAM POSITIF

Fahmi, Aliyah

doi:10.31289/biolink.v6i2.2814

Cited by 4 publications

(8 citation statements)

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“…Then, it was filtered using filter paper and then re-immersed on the filtered residue for 1 days with the same treatment as the previous stage. The macerate was evaporated in average temperature at 78 0 C to obtain a thick green tea ethanol extract (Fahmi, 2020).…”

Section: Sample Preparation and Extractionmentioning

confidence: 99%

“…P. aeruginosa colonies were taken using a round needle that had been sterilized and implanted on the surface of the nutrient media so that it was slanted by scratching and then incubated at 37 0 C for 24 hours (Fahmi, 2020).…”

Section: Preparation Of Bacterial Culture Stockmentioning

confidence: 99%

“…aeruginosa isolates were grown as colonies and then taken from the culture stock using a sterilize ose needle first, after that suspended in a test tube containing 10 mL of nutrient broth media and incubated at 37 0 C for 24 hours which means fulfill to the standard (Mc. Farland) for the turbidity (Fahmi, 2020).…”

Section: Bacterial Inoculum Makingmentioning

confidence: 99%

“…Disc paper that has been given with the concentration of each test solution that placed on the surface of the media has solidified then, incubated at 37 0 C during 18 hours until 24 hours. Observed and measured the inhibition diameter which formed using a caliper (Fahmi, 2020).…”

Section: Inhibitory Efficacymentioning

confidence: 99%

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Green Tea Ethanol Extract Efficacy Against Pseudomonas Aeruginosa

Fahmi¹,

Syukur

Chaidir

et al. 2022

BIOLINK

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Research about green tea ethanol extract efficacy against Pseudomonas aeruginosa has been done. The green tea leaves were sourced from tea distributor in Sidamanik District, North Sumatra, Indonesia. Green tea leaves extract was obtained by maceration technique in which green tea leaves were soaked with ethanol 96% for 24 hours. After that, the filtrate was concentrated to be thick extract with no liquid content again.Â After that, the concentrated extract was made by 5%, 10% and 15% as extract variations. The efficacy of green tea ethanol extract against Pseudomonas aeruginosaÂ was signed by increasing the inhibitory diameter that using disc method, in which the distilled water was used as negative blank. The results obtained for blanks, 5%, 10% and 15% extracts were 0; 1.85; 2.9 and 4.45 mm. The conclusion of this study is that the concentration of the extract is increasing proportionally to its inhibitory power, the higher of extract concentration is higher of its inhibitory activity against Pseudomonas aeruginosa. The conclusion described the green tea ethanol extract was effective to be antibacterial agent against Pseudomonas aeruginosa.

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Section: Sample Preparation and Extractionmentioning

confidence: 99%

Section: Preparation Of Bacterial Culture Stockmentioning

confidence: 99%

Section: Bacterial Inoculum Makingmentioning

confidence: 99%

Section: Inhibitory Efficacymentioning

confidence: 99%

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Green Tea Ethanol Extract Efficacy Against Pseudomonas Aeruginosa

Fahmi¹,

Syukur

Chaidir

et al. 2022

BIOLINK

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“…Sebelumnya, Peneliti telah melakukan penelitian terhadap ekstrak daun bawang batak dengan judul diantaranya 1). Fahmi, A. (2019)…”

Section: Pendahuluanunclassified

UJI ANTIBAKTERI MENGGUNAKAN PELARUT AQUADEST DARI TIGA VARIASI EKSTRAK DAUN BAWANG BATAK (Allium chinense G. Don)

Fahmi¹

2020

jons

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Penelitian dengan judul “Uji Antibakteri Menggunakan Pelarut Aquadest Dari tiga Variasi Ekstrak Daun Bawang Batak (Allium Chinense G. Don)“ telah dilakukan. Untuk Uji Antibakteri menggunakan metode difusi berpelarut aquadest dengan konsentrasi yang sama 0.25% diperoleh diameter zona hambat dari ekstrak metanol dan etil asetat total serta residu ekstrak etil asetat dengan bakteri gram positif Bacillus cereus adalah (17.6,22,11.6) mm dan Streptococcus mutans (18.5,24,12)mm dan untuk bakteri gram negatif Salmonella thypii (16.4,22.4,12.4)mm sehingga disimpulkan bahwa ketiga ekstrak daun bawang batak tersebut dapat dikembangkan sebagai zat antimikroba dimana yang paling kuat zona hambat dengan konsentrasi yang sama terdapat pada residu ekstrak etil asetat total diikuti oleh ekstrak metanol dan terakhir ekstrak etil asetat daun bawang batak (Allium chinense G. Don)

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Effectiveness of Batak Leeks Extract Against S. Mutans and E. Faecalis as Antibacterial and Antibiofilm Agent

OCTIARA,

ADINDA,

SEMBIRING

2024

Int J App Pharm

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Objective: S. mutans biofilms have become the main cause of dental caries. Meanwhile, E. faecalis biofilms cause endodontic infection. Batak leeks are native North Sumatra herb that contains antibacterial properties. This study aims to determine Batak leeks extract's antibacterial and antibiofilm effectiveness towards S. mutans and E. faecalis. Methods: This study was a laboratory experimental research with a posttest-only control group design. The sample size was 27, consisting of 3 samples each with concentrations of 0.78%; 1.56%; 3.25%; 6.25%; 12.5%; 25% and 50% Batak leek extract and 3 negative control samples and 3 positive. The Kirby-Bauer disc diffusion method was used to determine MIC, the streaking method to determine MBC and the Static Microtiter Plate Assays method for antibiofilm determination. Data analysis used Oneway ANOVA and Kruskall Wallis tests followed by post-hoc LSD and Mann-Whitney tests. Results: The results showed that Batak leeks extract contained MIC values at 0,78% concentration levels with an average inhibitory zone of 6,80±0,10 mm in S. mutans and E. faecalis. MBC values were undetected because there were no group capable of reducing bacteria at 98-98% and the highest antibiofilm reduction was found in 50% concentration extract with values of 85.58% for S. mutans and 90.29% for E. faecalis. Conclusion: It concluded that 0.78% concentration of Batak leeks extract has an antibacterial effect, but at 50% concentration was the most influential in inhibiting S. mutans and E. faecalis, and antibiofilm ability starts at the concentration level of 1.56%.

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UJI AKTIVITAS ANTIBAKTERI EKSTRAK METANOL DAUN BAWANG BATAK (Allium chinense G. Don) TERHADAP Streptococcus mutans DAN Bacillus cereus SEBAGAI BAKTERI GRAM POSITIF

Cited by 4 publications

References 0 publications

Green Tea Ethanol Extract Efficacy Against Pseudomonas Aeruginosa

Green Tea Ethanol Extract Efficacy Against Pseudomonas Aeruginosa

UJI ANTIBAKTERI MENGGUNAKAN PELARUT AQUADEST DARI TIGA VARIASI EKSTRAK DAUN BAWANG BATAK (Allium chinense G. Don)

Effectiveness of Batak Leeks Extract Against S. Mutans and E. Faecalis as Antibacterial and Antibiofilm Agent

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