Uji Aktivitas Antidiabetes Ekstrak  n-Heksan Kulit Buah Naga Merah (Hylocereus polyrhyzus)

Mahargyani, Wikan

doi:10.30870/educhemia.v4i1.3958

Cited by 7 publications

(8 citation statements)

References 2 publications

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“…Acarbose acts as a competitive antihyperglycemic agent, reversibly inhibiting α-amylase in the pancreas and binding to α-glucosidase enzymes in the intestinal membrane. The mechanism of this drug is to delay the hydrolysis of carbohydrates, disaccharides, and glucose absorption and inhibit the metabolism of sucrose to glucose and fructose [22].…”

Section: Resultsmentioning

confidence: 99%

Inhibitory Activity of the α-Glucosidase Enzyme by Albumin Isolated from Giant Gourami (Osphronemus Goramy), Rice Eel (Monopterus albus), and Mackerel Tuna (Euthynnus affinis)

Sa’adah¹,

Muhtadi²

2022

Atlantis Highlights in Chemistry and Pharmaceutical Sciences

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The giant gourami (Osphronemus goramy), rice eel (Monopterus albus), and mackerel tuna (Euthynnus affinis) are among the sources of protein and albumin that have been used to lower the blood glucose level of people with Diabetes Mellitus (DM). This study aims to determine the protein levels and albumin content of O. goramy, M. albus, and E. affinis and the inhibition activity of albumin against the α-glucosidase enzyme. This study employed an experimental method with accidental sampling. The protein content of fresh fish fillets was tested using the Kjeldahl method and extracted using a centrifuge to obtain albumin. Albumin levels were determined using a visible spectrophotometer. The inhibitory activity of albumin against α-glucosidase enzyme was tested using an ELISA reader. The results showed a significant difference between freshwater and seawater fish proteins, namely giant gourami of 13.91%, rice eel of 14.41%, and mackerel tuna of 30.55%. The highest albumin content was obtained from mackerel tuna (4.75 ± 0.04 g/100 mL), followed by giant gourami (3.61 ± 0.26 g/100 mL), and rice eel (2.38 ± 0.26 g/100 mL). The albumin showed no significant activity against the α-glucosidase enzyme.

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Section: Resultsmentioning

confidence: 99%

Inhibitory Activity of the α-Glucosidase Enzyme by Albumin Isolated from Giant Gourami (Osphronemus Goramy), Rice Eel (Monopterus albus), and Mackerel Tuna (Euthynnus affinis)

Sa’adah¹,

Muhtadi²

2022

Atlantis Highlights in Chemistry and Pharmaceutical Sciences

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“…Kemudian jika dibandingkan, bagian dari buahnya yang terbatas memiliki kemampuan untuk meredam radikal bebas 27,45±5,03 %. 7 Fitoestrogen merupakan salah satu senyawa polifenol non-steroid. Fitoestrogen kerap kali ditemukan pada tumbuhtumbuhan.…”

Section: Pendahuluanunclassified

The THE EFFECT OF ETHANOL EXTRACT FROM RED DRAGON FRUIT (HYLOCEREUS POLYRHIZUS) PEELS ON LIVER MDA (MALONDIALDEHYDE) LEVELS IN MENOPAUSE MODEL RAT

Savitri

2022

eum

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Menopause ditandai dengan terhentinya proses ovarium dalam memproduksi estrogen, sehingga sistem reproduksi wanita akan berhenti secara bertahap. Pada kondisi ini, akan terjadi penurunan fungsi metabolisme tubuh dan fungsi berbagai organ, salah satunya adalah liver. Hal ini disebabkan karena, selain berfungsi sebagai hormon, estrogen juga bekerja sebagai antioksidan endogen tubuh. MDA merupakan senyawa organik sebagai penanda terjadinya stres oksidatif tubuh. Pada kondisi menopause akan terjadi peningkatan stres oksidatif akibat ketidakseimbangan produksi radikal bebas dengan antioksidan yang dimiliki oleh tubuh. Sehingga pada menopause cenderung akan terjadi peningkatan kadar MDA. Peningkatan kadar MDA di liver akan menimbulkan berbagai gangguan pada liver, seperti: non alcoholic liver disease, fibrosis hati, dan keganasan pada hati. Subjek sampel dikelompokkan menjadi tiga sampel subjek, yang terdiri dari kelompok kontrol (P0), sampel uji dengan ekstrak etanol kulit dari buah naga merah dosis 60 mg/200grBB/hari (P2), kemudian sampel dengan ekstrak etanol kulit dari buah naga merah dosis 90 mg/200grBB/hari (P3). Didapat nilai rerata MDA liver pada subjek kelompok P0 adalah 10.20768 ± 8.85632, sekaligus menjadi rerata kadar MDA teringgi. Nilai rerata MDA liver pada subjek kelompok P1 adalah 2.98490 ± 2.41730, dan menjadi rerata kadar MDA serum terendah. Nilai rerata MDA liver pada subjek kelompok P2 adalah 3.33072 ± 2.73548. Antioksidan yang memiliki kesamaan struktur dengan fitoestrogen pun dipilih sebagai substitusi estrogen endogen. Salah satu bahan makanan kaya antosianin adalah kulit dari buah naga merah, sehingga diharapkan dengan pemberian ekstrak tersebut mampu menjadi antioksidan eksogen dalam terapi menopause. Kata kunci : MDA, Kulit Buah Naga Merah, Antioksidan

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“…Pada penelitian in vitro, inhibisi αglukosidase menjadi mekanisme pengujian yang telah banyak dilakukan dengan metode spektrofotometer UV-Vis [5][6][7]. Pengujian ini dilakukan dengan prinsip pengukuran kadar p-Nitrofenol (p-NP) yang merupakan produk hasil katalisis α-glukosidase terhadap substrat pnitrofenil α-D-glukopiranosida (PNPG) [9].…”

Section: Pendahuluanunclassified

Pengembangan Biosensor Berbasis Plastik Zona Mikro untuk Skrining Aktivitas Antidiabetes pada Ekstrak Tanaman Obat

Wati

Kuswandi

Pratoko

2021

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Research on the diabetes mellitus (DM) treatment has recently been carried out, especially on discovering antidiabetic agents derived from plants. This study aims to determine the fabrication of microzone plastic-based biosensors, determine the optimal sensor conditions, analysis characteristics, and compare the biosensor with the UV-Vis spectrophotometric method. The results showed that the response time was 15 minutes, the linear range was 500-40000 µg / mL (R = 0.9989). The limit of detection (LOD) was 1109.6 µg / mL, and the limit of quantification (LOQ) was 3698.8 µg / mL. Biosensor filled up the precision parameters with an RSD value of less than 3.7% and an accuracy with recovery in the range of 95-105%. The biosensor is stable in storage at 25 ° C for 270 minutes and at chiller temperature for three days. The antidiabetic activity of the biosensor was compared with the UV-Vis spectrophotometer using the Independent Sample T-test and showed insignificant differences between the two methods.

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Uji Aktivitas Antidiabetes Ekstrak n-Heksan Kulit Buah Naga Merah (Hylocereus polyrhyzus)

Cited by 7 publications

References 2 publications

Inhibitory Activity of the α-Glucosidase Enzyme by Albumin Isolated from Giant Gourami (Osphronemus Goramy), Rice Eel (Monopterus albus), and Mackerel Tuna (Euthynnus affinis)

Inhibitory Activity of the α-Glucosidase Enzyme by Albumin Isolated from Giant Gourami (Osphronemus Goramy), Rice Eel (Monopterus albus), and Mackerel Tuna (Euthynnus affinis)

The THE EFFECT OF ETHANOL EXTRACT FROM RED DRAGON FRUIT (HYLOCEREUS POLYRHIZUS) PEELS ON LIVER MDA (MALONDIALDEHYDE) LEVELS IN MENOPAUSE MODEL RAT

Pengembangan Biosensor Berbasis Plastik Zona Mikro untuk Skrining Aktivitas Antidiabetes pada Ekstrak Tanaman Obat

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