Candida albicans is fungi that cause 50-90% candidiasis in human. Candida albicans is commensal and opportunistic pathogen which interfere gastrointestinal, oropharynx, and female genitalia. Candida albicans resistant to standard antifungal due to repeated use of antifungal. Virgin coconut oil (VCO) contain fatty acid which effective as antifungal. The previous study showed the result of Kirby Bauer method on antifungal effect of 7.5% fatty acid in VCO were able to inhibit Candida albicans, this concentration is equal to nystatin 195 unit. Gas chromatography mass spectrometry showed the fatty acids are caproic acid (0.187%), cyclopropanepentanoic acid (1,12%), nonanoic acid (0.54%), lauric acid (32.73%), myristic acid (28.55%), palmitic acid (17.16%), oleic acid (14.09%), adna octadecanoic acid (5.68%). Saturated and unsaturated fatty acid in VCO are able to inhibit the growth of Candida albicans.

The inhibition from peel of red dragon fuits (Hylocereus polyrhizus) on α-glucosidase enzyme activity has been carried out. This study aims to determine the ability of peel extract as an inhibitor on enzymatic activity that occurs in glucose formation. Steps taken in this study include extraction of peel using ethanol and continued fractionation using n-hexane. N-hexane extract was tested using the mechanism of inhibition of α-glucosidase enzyme activity. Acarbose solution was used as a positive control in this test. The results showed that the dragon fruit extract of n-hexane fraction had IC 50 of 194.11 ppm, while the acarbose of 5.6 ppm. The smaller the IC 50 value indicates that the resistance is getting better.

Inflammation is the body’s reaction to the presence of infection, irritation or foreign substances as part of the body’s defense mechanisms. Public interest in treatment with natural medicines is increasing. One of the medicinal plants that can be used for anti-inflammatory treatment is the peel of the Ambon banana (Musa paradisiaca L.). This study employed the red blood cell membrane stabilization method which is widely used in research as a biochemical parameter for in vitro testing of anti-inflammatory activity. This was an experimental study that aimed to determine the anti-inflammatory activity of the peel extract of the Ambon banana (Musa paradisiaca L.) using the red blood cell membrane stabilization method. The percentage level of hemolysis that occurred when the extract was added indicated that the tested extract had anti-inflammatory properties. The stability of the red blood cell membrane of the peel extract with a concentration of 125 ppm was 1.09%, and was 12.40% with a concentration of 250 ppm, 17.36% with a concentration of 500 ppm, and 42.79% with a concentration of 1000 ppm. Based on these results, it can be concluded that the peel of the Ambon banana (Musa paradisiaca L.) has potential as an anti-inflammatory agent. Keywords: anti-inflammatory, banana peel, red blood cell membrane

Immobilization lipase on chitosan powder by cross linking technique has been carried out. The research was purposed to determine the optimum condition of immobilization process and catalytic activities of immobilized lipase. Glutaraldehyde was used as cross linking agent in immobilization lipase on chitosan powder. Immobilization was performed under the optimum conditions such as solubility pH of lipase, degree of deacetylation, mole ratio of chitosan and glutaraldehyde, and concentration of enzyme. Immobilized and free lipases were assayed their activity, thermal stability, reused ability, and kinetic parameters for transesterification reaction of palm oil with methanol. Result of the study showed that the optimum conditions of immobilization were occurred when lipase was dissolved in buffer phosphate at pH 6, mole ratio of chitosan and glutaraldehyde 4:1, and 5% for concentration of enzyme. The immobilized lipase has lower thermal stability but has better affinity and reused stability than the free lipase.

Total cholesterol is the composition of many substances including cholesterol, triglycerides, LDL cholesterol, and HDL cholesterol. Cholesterol examination is one of the most frequent tests required in the laboratory to monitor vascular and cardiovascular diseases. Most clinical pathology laboratories use photometer to perform clinical chemistry checks. Cholesterol testing can also be done with Point of Care Testing (POCT) which has a working principle of biosensor technology. This research method is experimental, using 40 samples that can represent normal and pathological levels. All samples will be checked for total cholesterol with a photometer of CHOD-PAP method and 3 POCT Lipid Pro. The results showed linear regression y = 0.955x + 1.8325 with R2 of 0.9955. The linear regression value is calculated by Total Error (TE), while the Total Error Allowable (TEa) cholesterol is 10%. The bias value is 0.31%, TE for normal level = 5.92% and TE for high pathological level = 3.00%, it can be stated the result of examination can be compared or accepted. The% TE value obtained is less than the TEa value of cholesterol. It can be concluded that the total cholesterol results examined by the photometer and LipidPro are comparable. For further research it is advisable to use a total cholesterol sample that has a value of more than 400 mg/dL.