UJI AKTIVITAS ANTIRADIKAL EKSTRAK ETANOL DAUN Elephantopus schaber L., Ocimum basilicum L.forma citratum Back., Graptophylum pictum Griff, dan Gynura procumbens Merr. DENGAN METODE DPPH (1,1- Difenil-2- Pikril Hidrazil) SERTA PENETAPAN KADAR FENOLIK TOT

Abstract

<jats:p>Daun Elephantopus schaber L. (tapak liman), Ocimum basilicum L.forma citratum Back., (selasih) Graptophylum pictum Griff (daun ungu), dan Gynura procumbens Merr. (sambung nyawa) memiliki kandungan kimia yang dapat berperan sebagai antiradikal, diantaranya adalah senyawa fenolik. Penelitian ini dilakukan untuk melihat korelasi kandungan fenolik total keempat daun terhadap aktivitas antiradikalnya. Sampel uji yaitu ekstrak etanol daun selasih, ungu, sambung nyawa, dan tapak liman ditentukan aktivitas antiradikal dengan menggunakan metode DPPH (1,1 difenil-2-pikrilhidrazil) kemudian dihitung nilai inhibitory concentration (IC50). Kandungan fenolik total ditentukan secara spektrofotometri menggunakan pereaksi Folin-Ciocalteu dan dihitung sebagai GAE (gallic acid equivalent) yaitu jumlah kesetaraan miligram asam galat dalam satu gram sampel. Penelitian menunjukkan ekstrak etanol daun selasih, ungu, sambung nyawa dan tapak liman memiliki aktivitas antiradikal dengan nilai IC50 berturut-turut 36,028; 54,998; 103,650; 252,857µg/mL dan kadar fenolik total berturut-turut 167,451; 147,064; 39,371 dan 20,539 mg/g sampel. Hasil penelitian secara keseluruhan menunjukkan ekstrak etanol daun selasih, ungu, sambung nyawa dan tapak liman berkorelasi positif terhadap aktivitas antiradikalnya. Kata kunci: Antiradikal, DPPH, IC50, fenolik total, Elephantopus schaber L., Ocimum basilicum L.forma citratum Back., Graptophylum pictum Griff, Gynura procumbens Merr.</jats:p>

Effect of Ethanol Extract of Basil Leaves (Ocimum basilicum) on the number of M2 Macrophages in the Kidneys of Mice (Mus musculus) Using the Unilateral Ureteral Obstruction (UUO) Method

“…15 Flavonoid chemicals can boost cell regeneration by destroying free radicals, providing a competitive substrate for unsaturated lipids in the membrane, and/or speeding up cell membrane repair mechanisms. 16 Flavonoids are natural antioxidants in basil leaves (Ocimum basilicum L.) 17 . Basil leaves (Ocimum basilicum L.) contain active chemicals in them, including essential oils, carbohydrates, phytosterols, alkaloids, phenolic compounds, tannins, lignin, starch, saponins, flavonoids, terpenoids, anthraquinones, apigenin flavones, luteolin, flavones oglucoside apigenin 7-O glucuronide, luteolin 7-O glucuronide, flavone C-glucoside orientin, molludistin and ursolic acid which function as antibacterial 18,19 .…”

The Hepatoprotective Effects of Basil Leaf (Ocimum sanctum L.) Extract on Paracetamol Induced Liver Damage in Male Rat

“…5 Medicinal plants are scientifically studied and researched, the results prove and support the fact that Biomedical Journal of Indonesia https://www.jurnalkedokteranunsri.id/index.php/BJI/index medicinal plants actually contain compounds and substances that are clinically proven to be beneficial to health 6 . Basil leaves are an example of a traditional plant that contains flavonoids as natural antioxidants that act as an antidote to free radicals 7 . Basil leaves have antioxidant compounds, namely phenolic compounds (flavonoids, phenolic acids, tocopherols), nitrogen compounds (chlorophyll derivatives, alkaloids, amines, and amino acids), and beta caratone.…”