Uji In Vitro Aktivitas Imunomodulator Minyak Atsiri Serai Dapur (Cymbopogon citratus) terhadap Proliferasi Sel Limfosit Mencit

Abstract

This study aims to determine the immunomodulatory activity of lemongrass essential oil on the proliferation of mouse lymphocyte cells in vitro. The method used is steam distillation to obtain citronella essential oil. Isolation of lymphocyte cells was obtained from the spleen organ of Swiss-Webster strain mice which was carried out aseptically. The lymphocyte cell proliferation test was carried out using the MTT Assay method and the absorbance was measured using an ELISA reader at 550 nm. The results showed that the lemongrass essential oil contained positive alkaloids, flavonoids, saponins, and steroids/triterpenoids with an essential oil yield of 0.043%. The higher the volatile oil concentration, the higher the Optical Density (OD) value obtained, but the OD increase at a 50 to 100 g/mL concentration was not significantly different. In conclusion, lemongrass essential oil has immunomodulatory activity against lymphocyte cell proliferation in mice tested in vitro.
 Keywords: Immunomodulator, Essential Oil, Lemongrass (Cymbopogon citratus), Immune System

Aktivitas Imunostimulan Ekstrak Metanol Biji Kurma (Phoenix dactylifera) secara In Silico Terhadap Reseptor GIF dan COX-2 serta Uji In Vitro melalui Proliferasi Sel Limfosit Mencit

“…Pembacaan nilai absorbansi dilakukan pada panjang gelombang 595 nm. Analisis dilakukan masing-masing sebanyak 3 kali ulangan [22].…”

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A Network Pharmacology of Lemongrass (Cymbopogon citratus) on COVID-19 Cases

“…The ease of lemongrass in tropical regions such as Indonesia makes lemongrass a plant that is easily found in the yards of Indonesian people's homes and is often used as a spice for cooking. The phytochemical composition of lemongrass includes tannins, saponins, anthraquinones, phenols, flavonoids and alkaloids (Ernis et al, 2020). Exploration of the activity of a compound of a plant can be done through three approaches: in vitro, in vivo, and in silico tests.…”