Ulcer Healing Mechanism of Ethanolic Extract of Talinium triangulare in Male Wistar Rats

Abstract

Talinium triangulare have been proven to offer promising antiulcer properties which could validate its"folkoric use for the treatment of gastric ulcer in various part of Nigeria. This study investigates the role of methanolic extract of Talinium triangulare (METT) in gastric ulcer healing and the possible mechanisms involved. Twenty-eight male wistar rats (160-180gm, n=7) were grouped into; A-control, B-ulcerated untreated, C-ulcerated treated with Omeprazole (20 mg/kg b.w.) and D-ulcerated treated with METT (100 mg/kg b.w.). Gastric ulcer was induced by injecting 0.2 MLs of 40% acetic acid into the glandular part of the stomach for 45 seconds after which it was withdrawn, and the stomach surface cleaned with normal saline. Stomach samples were collected by day 14 post ulceration and assessed for ulcer score; a section of it was fixed for histological evaluation and immunohistochemical (Avidin-Biotin Immunoperoxidase method) analysis. Stomach tissue homogenates were used for enzymatic activities. Results were subjected to statistical analysis using analysis of variance method. There was a significant reduction in the ulcer area of the METT treated group compared with other ulcerated treated and untreated groups. Malondialdehyde concentration of the gastric tissue homogenate was significantly lower in METT treated group compared to other groups. The METT treated group significantly increased the level of superoxide dismutase and catalase compared to other test groups. Histological study showed that only METT treated rats produces predominantly normal mucosa, METT treated rats also expressed CD31 (a marker of angiogenesis) and EGFR (a marker of proliferation) more than the other rats, while the expressions of Ki67 (proliferation) and p53 (Apoptosis) by METT group were not different from other groups. Methanolic extracts of Talinum triangulare accelerated the healing of gastric ulcers in rats probably through reduced oxidative stress, increased cell proliferation, and angiogenesis.

Gastroprotective Effects of Vitex Doniana Leaf Fractions Against Acetic Acid Induced Gastric Ulcer Model in Albino Rats

“…Conversely, Pereira et al [58] reported an increase in the number of proliferating cells when they assessed PCNA in rats treated with a bark extract of Tabebuia avellanedae after induction of chronic ulcers using acetic acid. The report of the present study was however similar to that published by Ajayi et al [59]. In their study, there was no difference in expression of Ki67 between rats treated for chronic ulcer using ethanolic extract of Talinium triangulare and the untreated ones.…”

“…As discussed earlier, alkaloids are one of the polyphenols known to alter blood flow and may have played a role in this case via a yet to be established mechanism [39]. The higher expression of PECAM-1 in this study is similar to the report by Ajayi et al [59] in which the treatment of chronic ulcers induced by acetic acid was performed with ethanolic extract of Talinium triangulare. They reported an increase in the expression of PECAM-1 in the gastric mucosa of treated rats compared to untreated.…”

Age-related changes in haematological parameters and biochemical markers of healing in the stomach of rats with acetic acid induced injury

“…Gastric ulcers were induced using acetic acid following the method described by Ajayi et al [ 29 ] with a little modification. There was no food in the animal's cages 24 h before the induction procedure, and then the stomach of each rat was exposed under anaesthesia [a mixture of xylazine (0.0005 ml/g b.w) and ketamine (0.0015 ml/g b.w)] by performing laparotomy through a midline epigastric incision.…”

Therapeutic potentials and pharmacological properties of Talinum triangulare (Jacq.) Willd. in the treatment and management of diabetes mellitus and other conditions

“…Peptic ulcer is a consequence of an imbalance between aggressive factors such as acid, pepsin, nonsteroidal anti-inflammatory medications and mucosal defence system (74,75). To evaluate the antiulcer activity of T. triangulare, leaves were harvested from the local garden at Faculty of Agricultural Sciences, LAUTech, Ogbomoso, Nigeria (75). The plants were scientifically identified, processed, and extracted according to known methods.…”