Uliginosibacterium sediminicola sp. nov., isolated from freshwater sediment

Abstract

Strain M1-21 is a Gram-stain-negative, strictly aerobic and short-rod-shaped bacterium, motile by means of a single polar flagellum; it was isolated from freshwater sediment in Korea. It grew at 10-40 °C (optimum 25 °C), pH 6.0-8.0 (optimum pH 7.0) and with 0-0.75 % (w/v) NaCl (optimal growth occurred in the absence of NaCl) on R2A agar, and it accumulated poly-β-hydroxybutyrate granules inside the cells. According to 16S rRNA gene sequence analysis, strain M1-21 showed highest sequence similarity with Uliginosibacterium gangwonense (94.7 %) and Uliginosibacterium paludis (94.4 %). Phylogenetic analysis of the 16S rRNA gene sequences revealed that strain M1-21 belongs to the genus Uliginosibacterium. The DNA G+C content of strain M1-21 was 61.9 mol%. The predominant respiratory quinone was ubiquinone-8. The major fatty acids (>10 % of the total) were C16 : 0 and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Strain M1-21 showed distinct phenotypic characteristics that differentiated it from species of the genus Uliginosibacterium. Based on these results, strain M1-21 represents a novel species of the genus Uliginosibacterium, for which the name Uliginosibacterium sediminicola sp. nov. is proposed. The type strain is M1-21 (=KACC 19271=JCM 32000).

Niveibacterium microcysteis sp. nov., isolated from a cyanobacterial bloom sample

“…The medium was bubbled with CO 2 and N 2 at an 8 : 2 ratio for 6 min in an anaerobic jar and optical density (OD 600 ) was measured at 0, 6 and 12 days. The growth of strain HC41 T on R2A medium at various temperatures (5,7,9,11,13,15,17,19,21,24,26,28,30,32,34,36,38,40,43,45,47 Table 1. Comparison of the phenotypic properties of strain HC41 T and the type strains of closely related species Strains: 1, HC41 T (this study); 2, Niveibacterium umoris KACC 17062 T ; 3, Uliginosibacterium gangwonense 5YN10-9 T .…”

“…Similarly, Uliginosibacterium species (i.e. close relatives of the genus Niveibacterium ) also exhibit Q-8 as their major isoprenoid quinone and C 16:0 and summed feature 3 as their primary cellular fatty acids [12, 13]. In this study, we isolate a novel Niveibacterium species from a cyanobacterial bloom sample collected at Hapcheon–Changnyeong weir (HCW) in the Republic of Korea in July 2018 and describe the genetic and phenotypical characterization of this novel bacterium.…”