2012
DOI: 10.1002/jssc.201100855
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Ultra‐high pressure LC determination of glucosamine in shrimp by‐products and migration tests of chitosan films

Abstract: Chitosan, a multiple applications molecule, was isolated from shrimp by-products by fermentation. The amount of chitosan in the solid fraction of the fermented extract was measured after its conversion in the respective glucosamine units. The procedure includes an acid hydrolysis (110 °C, 4 h with HCl 8 M) and a derivatization with 9-fluorenylmethyl chloroformate (Fmoc-Cl). Ultra-high-pressure liquid chromatography method was developed and optimized. Excellent peaks resolution was achieved in just 10 min. The … Show more

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Cited by 14 publications
(11 citation statements)
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“…The derivatisation of glucosamine with FMOC-Cl was adapted from a previous published study (19). Briefly, 10 µL of syringe filtered sample (Chromafil PET 0.20 µm, Macherey-Nagel) was added to 100 µL borate buffer (100 mM, pH 7.0) after which 100 µL of a FMOC-Cl solution in acetonitrile (650 ppm) was added.…”
Section: Optimisation Of the Glucosamine Derivatisation Uplc-ms Method And Chitin Hydrolysis 231 Derivatisation Conditionsmentioning
confidence: 99%
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“…The derivatisation of glucosamine with FMOC-Cl was adapted from a previous published study (19). Briefly, 10 µL of syringe filtered sample (Chromafil PET 0.20 µm, Macherey-Nagel) was added to 100 µL borate buffer (100 mM, pH 7.0) after which 100 µL of a FMOC-Cl solution in acetonitrile (650 ppm) was added.…”
Section: Optimisation Of the Glucosamine Derivatisation Uplc-ms Method And Chitin Hydrolysis 231 Derivatisation Conditionsmentioning
confidence: 99%
“…Resulting from a literature survey, it was opted to use 8.0 M HCl for the hydrolysis of the chitin standard into glucosamine (19,21). To investigate the dependence of time and temperature on the hydrolysis, a face-centered central composite design with one centerpoint was used.…”
Section: Chitin Hydrolysismentioning
confidence: 99%
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“…An RP HPLC with spectrophotometric detection techniques is often associated with the separation of ASs, but due to lack of chromophore in their structure and insufficient separation from other polar substances, pre‐ or postcolumn derivatization methods for selective and reproducible chromatographic separations have been employed. Anthranilic acid , 1‐phenyl‐3‐methyl‐5‐pyrazolone , phenyl isothiocyanate (PITC) , 9‐fluorenylmethoxycarbonyl salts (FMOC) , 1‐naphthyl isothiocyanate , o ‐phthalaldehyde (OPA) , or OPA/3‐mercaptopropionic acid have been reported as derivatization reagents for ASs. However, several problems are often related with most of the derivatization reagents, including elimination of excess reagent after reaction, stability of degradation products, or formation of dimers.…”
Section: Introductionmentioning
confidence: 99%
“…As GLcN is similar to glucose in structure without any efficient chromophores or fluorophores, some methods have been reported for the quantitation of GLcN in plasma, using HPLC coupled with UV or fluorescence detection following a pre‐column derivatization, which were not sensitive enough to determine GLcN in human plasma. Besides, the process of sample preparation was time‐consuming, and not suitable for routine assay for a large number of bio‐samples.…”
Section: Introductionmentioning
confidence: 99%