Ultra higher assessment of Tyrosine compound by coupling for biological samples

Abstract

The compound of Tyrosine (Tyr) is considered one of the most important aromatic amino acids for the human body, also it is very important and so vital to success for the process of neurotransmitter synthesis. The main aim of this paper was to improve and modified procedure for ultra-micro assessment of Tyrosine compound through a reaction of coupling between our target material Tyrosine and diazotized solution of 4-amino antipyrine in basic medium to yield a salt complex with a higher absorptivity at 455 nm. A group of parameters has been done in order to evaluate the typical conditions. The standard calibration curve has been done to the concentration range (8.09x10-7 -1.11x10-1 μg/mL), also the value for absorptivity of molar founded 1.7x103 L.mol-1.cm-1, besides we found that (R.S.D.) value was less than1.12% and the value of recovery it is 99.78%. Mole ratio appeared as 1:1 (Tyrosine: 4amino antipyrine), the value of the stability factor reached to 1.6x107L.mol-1. Our proposed procedure confirmed a group of merits such as simplicity, not need time, not necessarily providing expertise person to do this work and could be recommended for ultra-micro determination of tyrosine in all fields. This method could be properly validated for the assessment of tyrosine in biological samples.