Ultra-performance liquid chromatographic separation of geometric isomers of carotenoids and antioxidant activities of 20 tomato cultivars and breeding lines

Contact probe voltammetry for in situ monitoring of the reactivity of phenolic tomato (Solanum lycopersicum L.) compounds with ROS

“…Estimates of the TAP can be made upon determining the individual antioxidant constituents, usually using liquid chromatography [9][10][11][12], and from chemical-based assays such as the ABTS, DPPH and TEAC methodologies [13]. In all cases, the assays require more or less complex protocols involving an extraction step.…”

Food Safety: Food Analysis Technologies/Techniques

“…The generated ions are separated in the electrostatic field and then finally detected according to their mass-to-charge ratio (m/z). LC-MS has played important roles in the screening and identification of bioactive compounds such as polyphenols and carotenoids in fresh foods, and in functional foods and nutraceuticals (Paré and Bélanger, 1997;Li et al, 2012). There are different ionization methods, but electrospray ionization and atmospheric-pressure chemical ionization are two of the most frequently used methods, and both can be in either positive or negative mode (Hoffmann and Stroobant, 2013).…”

“…Many hydrophilic food components such as vitamin C, amino acids, phenolic compounds, and many bioactive foods are analyzed by reversedphase HPLC, whereas both normal and reversed HPLC are used for lipophilic compounds such as vitamin E and carotenoids (Nollet, 2012;Li et al, 2012). It is a convenient and widely used technology for sugar content, pesticide residues, amino acids, toxins, organic acids, lipids, vitamins, and various phytochemicals in foods.…”

“…It is worth noting that in the past several years, two revolutionary technology advancements, the development of ultrahigh pressure pumps and sub-2-micron packing particles, have led to the state-of-the-art ultrahigh performance liquid chromatography (UPLC or UHPLC) (Waksmundzka-Hajnos and Sherma, 2011;Xu, 2013;Li et al, 2012). UHPLC not only significantly reduces the sample size, analytical run time, and the solvent usage, but also produces exceptionally high sensitivity and better resolution.…”

Evaluation of Lipophilic Antioxidant Capacity and Lycopene Content in Brazilian Tomatoes

“…1 There is a great interest in the study of carotenoids from tomato samples, since a prominent deficiency of -Carotene may lead to diseases such as xerophthalmia and blindness, while regular intakes of lutein can reduce the probability of occurrence of macular degeneration, which is related to natural aging, and cataracts. 2 The presence of all these bioactive compounds justifies the labeling of tomatoes as functional foods. Actually, there are several studies on nutritional value and antioxidant capacity regarding tomato varieties from different locations, which were designed for different purposes.…”