Ultra performance liquid chromatography analysis to study the changes in the carotenoid profile of commercial monovarietal fruit juices

Delpino-Rius, Antoni; Eras, Jordi; Marsol‐Vall, Alexis; Vilaró, F.; Balcells, Mercè; Canela‐Garayoa, Ramon

doi:10.1016/j.chroma.2014.01.044

Cited by 51 publications

(40 citation statements)

References 52 publications

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“…These values revealed approximately 15% of carotenoid losses during the complete analytical procedure, including extraction, saponification and HPLC analysis. Recent studies [28][29][30] related to carotenoid recoveries including all analysis steps reported similar results. Stinco et al 28 reported recoveries of around 82% for (all-E)-β-carotene when saponification step was included.…”

Section: Methods Validationsupporting

confidence: 66%

“…In the same study, when samples were analyzed without alkaline hydrolysis the recovery values ranged from 92 to 107%. 28 Delpino-Rius et al 29 reported recoveries of 82% for (9Z)-neoxanthin and 75% for (all-E)-violaxanthin in samples spiked prior to extraction and followed by saponification. Inbaraj et al 30 reported recoveries of zeaxanthin, β-cryptoxanthin and β-carotene of 92, 92 and 87%, respectively, without saponification, while with saponification, recoveries dropped to 83, 74 and 74%, respectively.…”

Section: Methods Validationmentioning

confidence: 99%

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New Method for Carotenoid Extraction and Analysis by HPLC-DAD-MS/MS in Freeze-Dried Citrus and Mango Pulps

Petry¹,

Mercadante²

2017

J. Braz. Chem. Soc.

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Carotenoid analysis in citrus is still a challenge due to their complex carotenoid profile and matrix acidity, which can promote isomerization and epoxide-furanoid rearrangements of these compounds. A new method for carotenoid extraction from freeze-dried citrus was developed. Extraction was carried out by magnetic stirring using ethyl acetate and methanol as solvents and Na 2 CO 3 to neutralize organic acids. Liquid-liquid partition was achieved by centrifugation. Carotenoid quantification was accomplished by both UV-Vis and high-performance liquid chromatography-diode array detection (HPLC-DAD). Method repeatability (2.9 to 8.5%) and recovery (82 to 88%) were similar to reported values for procedures including all the carotenoid analysis steps. In 'Valencia' orange pulp, xanthophylls accounted for 98% of the total carotenoid content and the predominant carotenoids were (9Z)-violaxanthin, (all-E)-lutein and (9Z)-antheraxanthin. This method was further applied for carotenoid analysis in freeze-dried 'Tommy Atkins' mango pulp. The developed method is precise, accurate, fast and able to maintain epoxy-carotenoids stability during analysis.

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Section: Methods Validationsupporting

confidence: 66%

Section: Methods Validationmentioning

confidence: 99%

New Method for Carotenoid Extraction and Analysis by HPLC-DAD-MS/MS in Freeze-Dried Citrus and Mango Pulps

Petry¹,

Mercadante²

2017

J. Braz. Chem. Soc.

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“…Fat‐soluble micronutrients were extracted by using the slightly modified Folch method (Folch, Lees, & Sloane Stanley, 1957) and analyzed by previously reported UPLC method (Delpino‐Rius et al., 2014). The UPLC (ACQUITY UPLC I‐Class, Waters Co., Milford, MA, USA) system was equipped with a BEH C18 column (1.7 μm, 2.1 × 50 mm, Waters Co.), binary pump delivery system, autosampler, and photodiode array detector.…”

Section: Methodsmentioning

confidence: 99%

Black soybeans protect human keratinocytes from oxidative stress‐induced cell death

Yoon

Lee

Song

et al. 2018

Food Science & Nutrition

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Black soybeans are functional foods containing a variety of bioactives such as isoflavones, carotenoids, tocopherols, phenolic acid as well as anthocyanins. Here, we examined whether Cheongja#3 black soybean extract has a protective effect on oxidative stress‐induced cell death in human keratinocytes HaCaT. First, we identified fat‐soluble bioactives in three varieties of soybean extracts (Saedanbaek, Daechan, and Cheongja#3). In particular, black soybean Cheongja#3 had high amounts of lutein than other varieties. We demonstrated that Cheongja#3 extract reduced intracellular reactive oxygen species levels in HaCaT cells. Furthermore, Cheongja#3 protected cells from hydrogen peroxide (H2O2)‐induced oxidative stress and triggered cell death determined by cell viabilities and apoptotic caspase activities. Next, we identified the underlying mechanism is due to increased Nrf2 antioxidant system by Cheongja#3, thus increasing the expression of heme oxygenases (HO)‐1. These results indicated that Cheongja#3 soybean extract has protective role against oxidative stress by upregulating the Nrf‐2 antioxidant system in human keratinocyte HaCaT cells.

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“…The elution solvents were ACN:MeOH (7:3, v/v) (A) and 0.1% formic acid (B). Samples (10 µL) were eluted according to the linear gradient described by Delphino-Rius et al [15]. The runs were monitored at 450 and 650 nm.…”

Section: Identification and Quantification Of Carotenoids And Chloropmentioning

confidence: 99%

Determination of triterpenoids, carotenoids, chlorophylls, and antioxidant capacity in Allium ursinum L. at different times of harvesting and anatomical parts

Lachowicz

Oszmiański

Wiśniewski

2018

Eur Food Res Technol

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Triterpenoids, carotenoids, chlorophylls, and antioxidant capacity of individual parts of wild garlic at different times of harvesting were studied. Leaves, flowers, bulbs, and stems of Allium ursinum were used in this study. The contents of triterpenoids, carotenoids, and chlorophylls were determined by UPLC-PDA-Q/TOF-MS, and the antioxidant capacity was determined by ABTS and FRAP assay. A total of 21 compounds including 3 triterpenoids, 11 carotenoids, and 7 chlorophylls were identified in anatomical parts of A. ursinum in March and June 2016. These compounds present in individual anatomical parts of wild garlic were determination in this study for the first time ever. The average content of total triterpenoids ranged from 750.7 to 4159.5 mg/kg dry matter (dm) and that of carotenoids and chlorophylls ranged from 14.4 to 14,424.5 mg/kg dm for bulbs and leaves. The average level of the antioxidant capacity ranged from 37.1 to 91.7 µmol Trolox/g dm in bulbs and leaves, respectively. The leaves, flowers, and stems of wild garlic are a good source of health-promoting compounds. They can be used as functional food ingredients, dietary supplements, and additives.

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Ultra performance liquid chromatography analysis to study the changes in the carotenoid profile of commercial monovarietal fruit juices

Cited by 51 publications

References 52 publications

New Method for Carotenoid Extraction and Analysis by HPLC-DAD-MS/MS in Freeze-Dried Citrus and Mango Pulps

New Method for Carotenoid Extraction and Analysis by HPLC-DAD-MS/MS in Freeze-Dried Citrus and Mango Pulps

Black soybeans protect human keratinocytes from oxidative stress‐induced cell death

Determination of triterpenoids, carotenoids, chlorophylls, and antioxidant capacity in Allium ursinum L. at different times of harvesting and anatomical parts

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