Ultra Structural Changes in Rat Thyroid Gland Exposed to Fluoride

Shashi, A; Kumar, P. Shiva

doi:10.20546/ijcmas.2016.505.054

Cited by 2 publications

(2 citation statements)

References 4 publications

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“…It causes impairment in digestive tract, liver [3], brain [4], kidneys [5] and endocrine organs. Extremely high intake of fluoride may alter the function of pancreas [6], parathyroid [7], thyroid [8] and and also causes certain changes in the humoral profile [9].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Effect of Curcumin on Scanning Electron Microscopy of Rat Adrenal Gland in Experimental Fluorosis

Shashi

Tikka

2021

JPRI

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Aim: The present study was designed to investigate the therapeutic role of Curcumin against fluoride induced toxicity on adrenal gland of rats by using scanning electron microscopy (SEM). Methodology: Wistar albino rats were divided randomly into six groups The group I was administered with 1 ml of deionized water/kg b.w./day orally for 40 days. The Groups II and III were given 300 and 600 mg of NaF/kg b.w./day for the same period, respectively. The group IV was given 200 mg/kg b.w. of Curcumin for 20 days. The Groups V and VI were treated with 300 and 600 mg of NaF/kg b.w./day for 40 days respectively were post-treated with 200 mg of Curcumin for next 20 days. The animals were excised and adrenal tissue was taken out and processed for SEM. Results: The results revealed that rats exposed to 300 mg/kg b.w./day of NaF showed rough edges, numerous microvilli and damaged surface with crystal depositions. Also, numerous granules were distributed all over the surface. The rats treated with 600 mg/kg b.w./day of NaF showed decellularized adrenal tissue along with network of collagen fibres. Moreover, adrenal gland surface displayed abrasions and distorted cuboidal cells. The filopodia were prominent on the surface and wall of cavity possessed rough outline. After post-treatment with Curcumin, fluoridated adrenal gland of rats showed normal structure, reappearance of cuboidal cells on the surface as well as less number of microvilli and filopodia. Conclusion: The post-treatment with Curcumin possess therapeutic potential against NaF induced toxicity in adrenal gland of rats.

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Section: Introductionmentioning

confidence: 99%

Therapeutic Effect of Curcumin on Scanning Electron Microscopy of Rat Adrenal Gland in Experimental Fluorosis

Shashi

Tikka

2021

JPRI

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“…The thyroid gland is one of the most sensitive organs to excessive amounts of fluoride. Fluoride influences thyroid activity and development in a variety of ways [9].…”

Section: Introductionmentioning

confidence: 99%

Ameliorative Impacts of Dactyloctenium aegyptium and Parapholis incurva Ethanolic Extracts on Sodium Fluoride Induced Pathological Alterations in the Thyroid Gland in Male Rats

El-Sayed

Hussein²,

Elshamy³

et al. 2022

Egypt. J. Chem.

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The present study aimed to demonstrate the ameliorative impacts of Dactyloctenium aegyptium (D. aegyptium) and Parapholis incurva (P. incurva) alcoholic extracts on hormonal, histological, and ultrastructural changes in rat thyroid gland induced hypothyroidism by daily oral administration of sodium fluoride (NaF) (5 mg/kg) for 28 days. Forty-Two adult male albino rats were randomly divided into 6 equal groups (7 rats each): the control group, NaF group (5 mg/kg/d), D. aegyptium group (100 mg/kg/d), P. incurva group (100 mg/kg/d), NaF + D. aegyptium group and NaF + P. incurva group. At the end of the experiment, the animals were sacrificed and blood samples were subjected to hormonal assay for triiodothyronine (T3) and thyroxine (T4) and thyroid-stimulating hormone (TSH) levels. Malondialdehyde (MDA), reduced glutathione (GSH) and superoxide dismutase (SOD), and calcium were assessed. Specimens of thyroid gland tissues were processed for histological, histochemical, immunohistochemical and scanning electron microscopy examinations. Further chemical profiling of the both extracts were determined using HPLC. Phytochemically, 14 phenolic compounds including 10 phenolic and 4 flavonoids were identified from D. aegyptium while 13 phenolics were identified from P. incurva comprising 8 phenolic and 5 flavonoids. NaF induced significant alterations in hormonal (decrease in serum T3 and T4 levels and increase in serum TSH levels) and antioxidant status, as well as, calcium levels when compared with control group. Histopathological (many empty follicles lined by flat cells, hemorrhage and disrupted basement membrane), histomorphometric, histochemical, immunohistochemical and ultrastructural (thyroid calcification) alterations were also recorded, which all were markedly improved in groups that received D. aegyptium or P. incurva extract combined with NaF. D. aegyptium and P. incurva extracts are useful in combating thyroid gland calcification and toxicity induced by NaF due to their high flavonoids and phenolic compound content.

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Ultra Structural Changes in Rat Thyroid Gland Exposed to Fluoride

Cited by 2 publications

References 4 publications

Therapeutic Effect of Curcumin on Scanning Electron Microscopy of Rat Adrenal Gland in Experimental Fluorosis

Therapeutic Effect of Curcumin on Scanning Electron Microscopy of Rat Adrenal Gland in Experimental Fluorosis

Ameliorative Impacts of Dactyloctenium aegyptium and Parapholis incurva Ethanolic Extracts on Sodium Fluoride Induced Pathological Alterations in the Thyroid Gland in Male Rats

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