Ultra-structural Study by Transmission Electron Microscopy: Effect of Omega-3 on Ovary Cell Organelles after Experimental Induced Poly Cystic Ovary Syndrome

Abstract

Objectives: Polycystic ovary syndrome (PCOS) is the most frequent cause of female infertility, affecting about 4% to 8% of women in the age of procreation. For evaluation the protective effects of omega-3 polyunsaturated fatty acid on ovarian structure in experimental PCO induced by estradiol-valerat, this research was done. Materials and Methods: Wistar female rats (n=40) were allocated into four groups, one control (n=10) and three test groups (n=30), that one group received omega-3 (60 mg/rat/orally/daily), second and third groups were induced PCO by single injection of estradiol-valerate (16mg/ kg/ i.m), third group also received omega-3 (240 mg/kg) for 60 consequence days. Animals were kept in standard conditions. On day 60, the ovarian tissue of Rats in whole groups were removed and prepared for pathological analysis. Results: vacuolated area and rough endoplasmic reticulum expanded, de-granulated, disorganized were seen in PCO groups; however, these side effects decreased in the groups that received omega-3 significantly (p<0.05) in comparison to experiment groups and ovarian weights in PCO experimental decreased significantly (p<0.05). Conclusion: Results revealed that administration of omega-3 could significantly treat PCO. This suggested that polyunsaturated fatty acid could diminish negative side effects of PCO on ovary tissue.

Ovary stereological features and serum biochemical factors following induction of polycystic ovary syndrome with testosterone enanthate in mice: An experimental study

“…This could be due to the changes in the follicular fluid environment such as increased levels of androgens, triglycerides and insulin which may impact the oocyte volume ( 29 ). Our results also revealed that the mean thickness of zona pellucida significantly reduced in the PCOS group when compared to the control which is confirmed by the study conducted by Ouladsahebmadarek and Khaki who indicated a decrease in the thickness of zona pellucida and the distribution of microvilli in rats following estradiol valerate injection ( 30 ). Since oocyte and follicular cells are involved in the production of zona pellucid therefore the apoptosis of granulosa cells and follicular atresia could be the underlying cause for the reduction in ZP thickness ( 31 ).…”

Ameliorating effect of Brassica oleracea (broccoli) extract on the myocardial damage induced by polycystic ovary syndrome in the adult female albino rat

“…Accordingly, this work was set to investigate, for the first time up to the best of our knowledge, the reflection of cardiovascular risk factors associated with PCOS on the histopathological alterations in the cardiac muscle of adult female albino rats and whether broccoli extract can ameliorate these effects. In this work, we used a model in which PCOS was induced by a single intramuscular injection of estradiol valerate (EV) as employed by previous studies [29,34]. Estradiol valerate was reported to induce morphological changes in the ovaries like those of PCOS women.…”

“…Polycystic ovary syndrome (PCOS) was induced by a single intramuscular injection of estradiol valerate (EV) (16 mg/kg) [29] dissolved in 0.2 ml distilled water. During the experiment, the estrous cycle phases were monitored by the analysis of relative proportion of leukocytes, epithelial and cornified cells.…”

Comparison of the Effect of Letrozole Alone With Letrozole Plus N-Acetylcysteine on Pregnancy Rate in Patients With Polycystic Ovarian Syndrome: A Randomized Clinical Trial

“…According to the latest definition in 2003 (Rotterdam Criterion), PCOS has at least two criteria of three quantitative criteria, including the amount of menstrual bleeding (oligo menorrhea or amenorrhea), the clinical or biochemical symptoms of increased male hormones and sonographic findings suggesting polycystic ovary. Furthermore, fertility problems such as infertility, bleeding caused by dysfunction, endometrial carcinoma, and postmenopausal menopause are more common in patients with PCOS depression, low self-esteem, anxiety, and metabolic disorders such as obesity, impaired glucose tolerance, type 2 diabetes, lipid disorders, and hypertension suffer (7)(8)(9).…”