Ultra-trace determination of Se in sediments by electrothermal vaporizer-inductively coupled plasma-mass spectroscopy: use of the ETV as a thermochemical reactor

Lam, Joseph W. H.; Sturgeon, Ralph E.; McLaren, J. W.

doi:10.1016/s0584-8547(98)00255-9

Cited by 17 publications

(9 citation statements)

References 33 publications

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“…The Se(IV) signal in ion chromatographic assay may be severely affected by chloride, carbonate and nitrate; the Se(VI) peak may be obscured by the presence of sulfate [5,19]. The Se(IV) signal in ion chromatographic assay may be severely affected by chloride, carbonate and nitrate; the Se(VI) peak may be obscured by the presence of sulfate [5,19].…”

Section: Chromatographic Conditionsmentioning

confidence: 99%

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Ion-chromatography of inorganic selenium species with a preliminary preconcentration step

2003

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The essential as well as toxic character of selenium is related to its chemical forms and oxidation states. This study presents a method for the determination of Se(IV) and Se(VI) in aqueous solution by ion chromatography with UV detection. Several chromatographic parameters, including mobile phase concentration, pH and methanol content were optimised. The influence of other anions commonly present in water samples was also tested. The best results were obtained on a Hamilton PRP-X-IO0 column with a 1 mM phthalate eluent at pH 6 with 2.5% methanol. The hnear cahbration range was from 0.5 to 20 mg L 1. LOD were 60 and 40 I~g 9 L 1 for Se(IV) and Se(VI), respectively. Because this range was not sufficient for the determination of selenium in natural samples, a preconcentration step using solid-phase extraction with an AI203 microcolumn was added for the enrichment of the analytes. To improve the sensitivity and selectivity of the measurements, electrothermal AAS was also used as a detection method. This method was applied to the determination of selenium in thermal waters and extracts of pharmaceutical supplements.

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Section: Chromatographic Conditionsmentioning

confidence: 99%

“…L 1 when electrothermal vaporization was used as a thermochemical reactor [19] and 0.3 ixg 9 L 1 with a hexapole collision cell [20]. Even the total concentration of selenium is often close to analytical detection limits; the LOD values for selenium analysis reported up to now using the ICP-MS method were 1 ixg.…”

Section: Introductionmentioning

confidence: 97%

Ion-chromatography of inorganic selenium species with a preliminary preconcentration step

2003

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“…7 The proteins were subsequently derivatized by treatment with iodoacetic acid, as previously described, 8 in order to protect the side-chains containing selenium from oxidation. The latter solution, subjected to electrophoresis, contained 0.42 mg of GSH-Px mL 21 or 1.54 ng Se mL 21 , since GSH-Px consists of four 22612 Da monomers, 9 each of which contains one selenocysteine amino-acid. The yeast proteins solution contained 4.4 ng Se mL 21 .…”

Section: Sample Preparationmentioning

confidence: 99%

“…The latter solution, subjected to electrophoresis, contained 0.42 mg of GSH-Px mL 21 or 1.54 ng Se mL 21 , since GSH-Px consists of four 22612 Da monomers, 9 each of which contains one selenocysteine amino-acid. The yeast proteins solution contained 4.4 ng Se mL 21 .…”

Section: Sample Preparationmentioning

confidence: 99%

Detection and quantification of selenium in proteins by means of gel electrophoresis and electrothermal vaporization ICP-MS

Chéry

Chassaigne

Verbeeck

et al. 2002

J. Anal. At. Spectrom.

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A method for the separation of selenium-containing proteins and subsequent detection and quantification of selenium was developed. First, the proteins are fractionated by means of sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE); subsequently, the bands of the gel, containing the proteins, are analyzed by electrothermal vaporization (ETV)-ICP-MS to detect and quantify selenium. External standardization with the use of an internal reference (Te) was applied. A detection limit of ca. 40 pg Se per band and a recovery of ca. 98% were obtained. A single measurement is accomplished in less than 4 min and thus a gel lane after a separation of ca. 1.5 h, can be entirely analyzed with ETV-ICP-MS in 3.5 h. If limited to 10 bands, a gel lane is analyzed in 2 h (calibration included). The analysis is directly carried out on the stained gel, without blotting, which makes the analysis even more practical. This method was optimized using the selenoprotein glutathione peroxidase as model. Then, it was applied to the fractionation of proteins from a selenium-yeast candidate reference material. The reconstructed Se electropherograms are presented and compared with the stained gels. The major advantages of this method are the high resolution of the protein fractionation and the straightforward quantification of selenium

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“…This strategy has been applied to the determination of various trace elements in several previous studies. 16,17 Addition of certain organic compounds promote the atomization of analyte prior to the vaporization of bulk matrix, thus facilitating the temporal separation of the analyte and the matrix leading to less interference effects. For example, EDTA was used as the chemical modier to improve the signals of Cd, Hg and Pb in a previous ETV-ICP-MS application.…”

Section: Introductionmentioning

confidence: 99%

Multi-element analysis of sunscreens using slurry sampling electrothermal vaporization inductively coupled plasma mass spectrometry

Jiang

Chen

et al. 2014

J. Anal. At. Spectrom.

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Ultra-trace determination of Se in sediments by electrothermal vaporizer-inductively coupled plasma-mass spectroscopy: use of the ETV as a thermochemical reactor

Cited by 17 publications

References 33 publications

Ion-chromatography of inorganic selenium species with a preliminary preconcentration step

Ion-chromatography of inorganic selenium species with a preliminary preconcentration step

Detection and quantification of selenium in proteins by means of gel electrophoresis and electrothermal vaporization ICP-MS

Multi-element analysis of sunscreens using slurry sampling electrothermal vaporization inductively coupled plasma mass spectrometry

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