Ultrabright Organic Dots with Aggregation‐Induced Emission Characteristics for Real‐Time Two‐Photon Intravital Vasculature Imaging

Ding, Dan; Goh, Chi Ching; Feng, Guangxue; Zhao, Zujin; Liu, Jie; Liu, Rongrong; Tomczak, Nikodem; Geng, Junlong; Tang, Ben Zhong; Ng, Lai Guan

doi:10.1002/adma.201301938

Cited by 263 publications

(213 citation statements)

References 35 publications

Supporting

Mentioning

210

Contrasting

Unclassified

Order By: Relevance

“…26 As shown in Figure 1, P2 shows weak fluorescence with an emission maximum at 505 nm due to its good solubility in DMSO solution. With gradually addition of water into DMSO (f w <80%), the PL intensity of P2 is dramatically enhanced, showing an obviously AIE effect.…”

Section: Synthesis and Characterization Of P2mentioning

confidence: 95%

Conjugated polymer nanoparticles with aggregation induced emission characteristics for intracellular Fe³⁺ sensing

Yang

Luo

et al. 2016

J. Polym. Sci. Part A: Polym. Chem.

View full text Add to dashboard Cite

In this article, a novel zwitterionic conjugated polyelectrolyte containing tetraphenylethene unit was synthesized via Pd-catalyzed Sonogashira reaction. The resulting polymer (P2), which exhibited typical aggregation-induced emission (AIE) properties, was weakly fluorescent in dilute DMSO solution and showed bright fluorescence emissions when aggregated in DMSO/water mixtures or fabricated into conjugated polymer nanoparticles (CPNs). The nanoparticles from P2 could be prepared by reprecipitation method with an average diameter around 23 nm. Notably, the cell-staining efficiencies of lipid-P2 nanoparticles could be enhanced with lipid encapsulation and these nanoparticles were endocytosed via caveolaemediated and clathrin-mediated endocytosis pathways. Furthermore, the lipid-P2 nanoparticles with low cytotoxicity, high photostability and efficient cell staining ability could be employed for in vitro detection of Fe 31 ions in A549 cells.

show abstract

Section: Synthesis and Characterization Of P2mentioning

confidence: 95%

Conjugated polymer nanoparticles with aggregation induced emission characteristics for intracellular Fe³⁺ sensing

Yang

Luo

et al. 2016

J. Polym. Sci. Part A: Polym. Chem.

View full text Add to dashboard Cite

show abstract

“…[24,25] To date, a large number of new AIE luminescent dyes including silole, tetraphenylethene (TPE), triphenylamine (TPA), 9,10-distyrylanthrancene (DSA), 1,4-distyrylbenzene (DSB) and their derivatives have been designed and explored for different applications. [25][26][27][28][29][30][31][32] However, most of AIE luminescent dyes are not soluble in physiological solution on account of their inherent hydrophobic features, which is a great challenge for their biological application. A general adopted strategy is the encapsulation of these hydrophobic dyes into or conjugation with other hydrophilic components to form amphiphilic block copolymers, which can self-assemble into nanoparticles in aqueous solution.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Amphiphilic Hyperbranched AIE‐active Fluorescent Organic Nanoparticles and Their Application in Biological Application

Wang

Xü

et al. 2015

Macromolecular Bioscience

View full text Add to dashboard Cite

Aggregation-induced emission (AIE) dyes have recently attracted much attention for biomedical applications for their remarkable AIE properties. However, the hydrophobic nature of AIE dyes made them difficult to be dispersed in physiological solution and problematic for biomedical application directly. Great efforts have been made to overcome this problem, and different strategies for preparation of water dispersible AIE based nanoprobes had been explored previously. However, a facile and effective strategy is still highly desirable and of great importance for the biomedical applications of AIE dye based on nanoprobes. In this work, the fabrication of amphiphilic hyperbranched fluorescent organic nanoparticles with a core-shell structure based on an AIE dye [tetraphenylethene acrylate (TPE-O-E)] and a hyperbranched polyamino compound [polyethylene imine (PEI)] through Michael addition reaction is described for the first time. The AIE dye as well as the final product PEI-TPE-O-E was characterized in detail by a number of techniques. To test their biomedical application potential, the cell viability as well as cell imaging properties of the PEI-TPE-O-E was also examined. The results showed that the PEI-TPE-O-E organic nanoparticles presented high water dispersiblity, ultrabright fluroerescence, low cytotoxicity and excellent biocompatibility, making them promising for biological imaging and gene delivery applications.

show abstract

“…al. [40] reported the synthesis of ultrabright polymer-based supramolecular AIE nanoparticles with small size (ca. 33 nm), which showed a high two-photon absorption cross-section, colloidal stability, excellent photostability, low in vivo toxicity and used for realtime intravital two-photon imaging of blood vessels.…”

Section: Fluorescencementioning

confidence: 99%

Smart Supramolecular Nanosystems for Bioimaging and Drug Delivery

Yang

Wang

2014

Chin. J. Chem.

View full text Add to dashboard Cite

The application of smart supramolecular nanosystems in biomedicine increases rapidly and offers promising prospects for disease diagnostics and therapeutics. Supramolecular nanosystems such as liposomes, micelles, organic nanoaggregates and metallic nanostructures etc. have been widely explored as diagnostic/therapeutic tools. Here, we review the recent advances in supramolecular nanosystems with different built-in reporters, e.g., fluorescent, magnetic and photoacoustic signals for bioimaging. In addition, the substantial progress of supramolecular nanosystems as drug delivery carriers for cancer therapy, including chemotherapy, photothermal and photodynamic therapies is also summarized.

show abstract

Ultrabright Organic Dots with Aggregation‐Induced Emission Characteristics for Real‐Time Two‐Photon Intravital Vasculature Imaging

Cited by 263 publications

References 35 publications

Conjugated polymer nanoparticles with aggregation induced emission characteristics for intracellular Fe³⁺ sensing

Conjugated polymer nanoparticles with aggregation induced emission characteristics for intracellular Fe³⁺ sensing

Synthesis of Amphiphilic Hyperbranched AIE‐active Fluorescent Organic Nanoparticles and Their Application in Biological Application

Smart Supramolecular Nanosystems for Bioimaging and Drug Delivery

Contact Info

Product

Resources

About

Ultrabright Organic Dots with Aggregation‐Induced Emission Characteristics for Real‐Time Two‐Photon Intravital Vasculature Imaging

Cited by 263 publications

References 35 publications

Conjugated polymer nanoparticles with aggregation induced emission characteristics for intracellular Fe3+ sensing

Conjugated polymer nanoparticles with aggregation induced emission characteristics for intracellular Fe3+ sensing

Synthesis of Amphiphilic Hyperbranched AIE‐active Fluorescent Organic Nanoparticles and Their Application in Biological Application

Smart Supramolecular Nanosystems for Bioimaging and Drug Delivery

Contact Info

Product

Resources

About

Conjugated polymer nanoparticles with aggregation induced emission characteristics for intracellular Fe³⁺ sensing

Conjugated polymer nanoparticles with aggregation induced emission characteristics for intracellular Fe³⁺ sensing