Ultrafast combined dynamics of Förster resonance energy transfer and transient quenching in cationic polyfluorene/fluorescein-labelled single-stranded DNA complex

Kim, Inhong; Kyhm, Kwangseuk; Kang, Mijeong; Woo, Han Young

doi:10.1016/j.jlumin.2014.01.042

Cited by 7 publications

(12 citation statements)

References 25 publications

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“…By virtue of their light-harvesting properties, CPEs have also been utilized as photosensitizers in solar cells , and fluorescent sensors. − The basis of the successful utilization of CPEs in optoelectronic applications is their semiconducting properties, including their large optical densities and high emission intensities for fluorescence resonance energy transfer . In particular, key features of CPEs for solar cell applications are their flexibility and their simple, large-scale, and low-cost fabrication. , Another advantage is that the functional groups of the side chains can be ionic or polar moieties, allowing the modification of not only the solubility of the CPEs in water and other polar solvents but also their intermolecular interactions, energy levels, and redox potentials, which determine electronic coupling and the rate of electron transfer at the donor–acceptor interface. , …”

Section: Introductionmentioning

confidence: 99%

Bimolecular Excited-State Electron Transfer with Surprisingly Long-Lived Radical Ions

et al. 2015

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Section: Introductionmentioning

confidence: 99%

Bimolecular Excited-State Electron Transfer with Surprisingly Long-Lived Radical Ions

et al. 2015

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“…The theoretical FRET efficiency equation, which is only determined by the FRET term, could not be applied on a practical FRET system because of fluorescence quenching. Various exciton deactivation processes such as charge transfer or carrier trapping including self-quenching drive fluorescence quenching 76–79 . Moreover, the presence of charged fluorescence quenchers can lead to the transformation of the overall dipole distribution.…”

Section: Resultsmentioning

confidence: 99%

Intermolecular distance measurement with TNT suppressor on the M13 bacteriophage-based Förster resonance energy transfer system

Kim

Song

Kim

et al. 2019

Sci Rep

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An M13 bacteriophage-based Förster resonance energy transfer (FRET) system is developed to estimate intermolecular distance at the nanoscale using a complex of CdSSe/ZnS nanocrystal quantum dots, genetically engineered M13 bacteriophages labeled with fluorescein isothiocyanate and trinitrotoluene (TNT) as an inhibitor. In the absence of trinitrotoluene, it is observed that a significant spectral shift from blue to green occur, which represents efficient energy transfer through dipole-dipole coupling between donor and acceptor, or FRET-on mode. On the other hand, in the presence of trinitrotoluene, the energy transfer is suppressed, since the donor-to-acceptor intermolecular distance is detuned by the specific capturing of TNT by the M13 bacteriophage, denoted as FRET-off mode. These noble features are confirmed by changes in the fluorescence intensity and the fluorescence decay curve. TNT addition to our system results in reducing the total energy transfer efficiency considerably from 16.1% to 7.6% compared to that in the non-TNT condition, while the exciton decay rate is significantly enhanced. In particular, we confirm that the energy transfer efficiency satisfies the original intermolecular distance dependence of FRET. The relative donor-to-acceptor distance is changed from 70.03 Å to 80.61 Å by inclusion of TNT.

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“…We have assumed that the intrinsic optical properties of constituents are unaffected or unchanged by the complexation and the addition of new molecules in order to simplify our understanding on phenomenological dynamics. Therefore, total energy transfer will be determined by the competition between energy harvesting one-step FRET and energy-wasting PCT because both processes have an intermolecular distance-dependent nature: FRET rate (kFRET) is inversely proportional to the sixth power of the donor–acceptor distance (~1/RDA6), while PCT rate (kPCT) has an exponential distance dependence (kPCT∝e−RDA) [39].…”

Section: Resultsmentioning

confidence: 99%

“…kF=krad+knonrad=1τrad+1τnonrad where τrad and τnonrad represent radiative and non-radiative decay time, respectively. Since the fluorescence intensity (I) is proportional to the exciton population (N), the time-dependent fluorescence decay can be characterized by a differential equation form of exponential function [39,45]. I(t)=80%true∫N(t)dt dN(t)dt=normalg−N(t)τint where g indicates carrier (exciton) generation function by excitation light.…”

Section: Resultsmentioning

confidence: 99%

Two-Step Energy Transfer Dynamics in Conjugated Polymer and Dye-Labeled Aptamer-Based Potassium Ion Detection Assay

et al. 2019

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We recently implemented highly sensitive detection systems for photo-sensitizing potassium ions (K+) based on two-step Förster resonance energy transfer (FRET). As a successive study for quantitative understanding of energy transfer processes in terms of the exciton population, we investigated the fluorescence decay dynamics in conjugated polymers and an aptamer-based 6-carboxyfluorescein (6-FAM)/6-carboxytetramethylrhodamine (TAMRA) complex. In the presence of K+ ions, the Guanine-rich aptamer enabled efficient two-step resonance energy transfer from conjugated polymers to dyed pairs of 6-FAM and TAMRA through the G-quadruplex phase. Although the fluorescence decay time of TAMRA barely changed, the fluorescence intensity was significantly increased. We also found that 6-FAM showed a decreased exciton population due the compensation of energy transfer to TAMRA by FRET from conjugated polymers, but a fluorescence quenching also occurred concomitantly. Consequently, the fluorescence intensity of TAMRA showed a 4-fold enhancement, where the initial transfer efficiency (~300%) rapidly saturated within ~0.5 ns and the plateau of transfer efficiency (~230%) remained afterward.

show abstract

Ultrafast combined dynamics of Förster resonance energy transfer and transient quenching in cationic polyfluorene/fluorescein-labelled single-stranded DNA complex

Cited by 7 publications

References 25 publications

Bimolecular Excited-State Electron Transfer with Surprisingly Long-Lived Radical Ions

Bimolecular Excited-State Electron Transfer with Surprisingly Long-Lived Radical Ions

Intermolecular distance measurement with TNT suppressor on the M13 bacteriophage-based Förster resonance energy transfer system

Two-Step Energy Transfer Dynamics in Conjugated Polymer and Dye-Labeled Aptamer-Based Potassium Ion Detection Assay

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