Ultrafast dynamics in a nanocage of enzymes: Solvation and fluorescence resonance energy transfer in reverse micelles

Validation and Divergence of the Activation Energy Barrier Crossing Transition at the AOT/Lecithin Reverse Micellar Interface

“…Hence, it is clear that, at 409 nm excitation, only those populations of C-500 molecules are excited which are in the polar environments of the RM. 29 Upon excitation of the probe at 409 nm, its emission is found to peak at 495 nm (Figure 3a), confirming the location of C-500 in the close vicinity of the RM interface. The absorption spectrum of C-500 shows a blue shift at elevated temperatures, which could reflect that the immediate environments of C-500 are becoming relatively less polar indicating dye migration from interface to the nonpolar isooctane side.…”

“…The red edge excitation wavelength (409 nm) used in our experiment only excites those populations of C-500 which are in the close vicinity of the water/surfactant interface, which is also evidenced from the earlier literature. 29 The advantages of red edge excitation has been investigated in the recent literature, 44,45 which indicates that the red edge excitation of the probe C-500 particularly excites only the interface bound populations. The C(t) curves of C-500 in w 0 ) 10 RM obtained at 293 and 343 K are presented in Figure 4b.…”

Enhanced permeation, leaf retention, and plant protease inhibitor activity with bicontinuous microemulsions

“…The WIB BCME itself has no adverse effects on the growth of the chickpea plant. BCME-enabled encapsulation and delivery opens newer avenues for protein and peptide delivery, previously limited to reverse micellar systems [17][18][19][20][21][22]. The bicontinuous domains have been previously reported to stabilize proteins and enzymes and have been envisaged as media for enzymatic reactions [13,14].…”

“…BCME systems have been previously explored for drug delivery and shown to preserve the stability and enzymatic activity of protein [4,8,9,[11][12][13][14][15][16]. However, unlike reverse micellar microemulsions, encapsulation of biocolloids such as proteins in BCMEs has been explored to a much lesser extent [17][18][19][20][21][22]. Permeation and transport of proteins enabled by BCMEs has unique applications in the development of efficient surface formulations.…”

Interaction of ANS with human serum albumin under confinement: Important insights and relevance

“…The surfactants (here sodium bis(2-ethylhexyl) sulfosuccinate (AOT)) beyond the critical micelle concentration self-assemble in a manner such that the polar head groups point inwards (away from the organic solvent) forming a polar core. The latter can solubilize relatively large quantities of water and is often characterized by the parameter w 0 , which is defined as [H 2 O]/ [Surfactant] and is regarded as the signature of the water loading capacity of that specific reverse micelle system [12]. The water inside the reverse micelle not only has the tendency to solubilize proteins, enzymes, amino acids and DNA but also is known to help retain their biological activity [13][14][15][16].…”