Ultrafast infrared chemical imaging of live cells

Amrania, Hemmel; McCrow, Andrew; Matthews, Mary; Kazarian, Sergei G.; Kuimova, Marina K.; Phillips, Chris C.

doi:10.1039/c0sc00409j

Cited by 27 publications

(20 citation statements)

References 10 publications

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“…A comparison of IR imaging with a laser and thermal source has not been reported in terms of spectral or spatial performance, though spectra were found to be in reasonable agreement in qualitative terms. 137 In addition to the optical and performance issues discussed for QCLs, cost is an additional factor in these systems. While the compact packaging and ability to use the same material for a variety of applications and wavelengths exists for QCLs, indirect production of mid-IR light inherently requires a larger capital cost and complexity of instrumentation for phase matching.…”

Section: Data Recording: Instrumentationmentioning

confidence: 99%

Infrared Spectroscopic Imaging: The Next Generation

2012

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Infrared (IR) spectroscopic imaging seemingly matured as a technology in the mid-2000s, with commercially successful instrumentation and reports in numerous applications. Recent developments, however, have transformed our understanding of the recorded data, provided capability for new instrumentation, and greatly enhanced the ability to extract more useful information in less time. These developments are summarized here in three broad areas— data recording, interpretation of recorded data, and information extraction—and their critical review is employed to project emerging trends. Overall, the convergence of selected components from hardware, theory, algorithms, and applications is one trend. Instead of similar, general-purpose instrumentation, another trend is likely to be diverse and application-targeted designs of instrumentation driven by emerging component technologies. The recent renaissance in both fundamental science and instrumentation will likely spur investigations at the confluence of conventional spectroscopic analyses and optical physics for improved data interpretation. While chemometrics has dominated data processing, a trend will likely lie in the development of signal processing algorithms to optimally extract spectral and spatial information prior to conventional chemometric analyses. Finally, the sum of these recent advances is likely to provide unprecedented capability in measurement and scientific insight, which will present new opportunities for the applied spectroscopist.

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Section: Data Recording: Instrumentationmentioning

confidence: 99%

Infrared Spectroscopic Imaging: The Next Generation

2012

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“…The aforementioned properties of these materials motivated a variety of experimental [6-8, 11, 12, 14, 15, 22-27] as well as theoretical studies [3,9,9,10,13,[16][17][18][19][20][21][28][29][30][31][32][33][34][35] with the latter utilizing computational techniques of varying sophistication, ranging from the tight binding and the empirical pseudopotential methods to ab-initio density functional theory (DFT).…”

Section: Introductionmentioning

confidence: 99%

Electronic, structural, and elastic properties of metal nitrides XN (X = Sc, Y): A first principle study

Ekuma¹,

Bagayoko²,

Jarrell³

et al. 2012

AIP Advances

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We utilized a simple, robust, first principle method, based on basis set optimization with the BZW-EF method, to study the electronic and related properties of transition metal mono-nitrides: ScN and YN. We solved the KS system of equations self-consistently within the linear combination of atomic orbitals (LCAO) formalism. It is shown that the band gap and low energy conduction bands, as well as elastic and structural properties, can be calculated with a reasonable accuracy when the LCAO formalism is used to obtain an optimal basis. Our calculated, indirect electronic band gap (EΓ−Xg) is 0.79 (LDA) and 0.88 eV (GGA) for ScN. In the case of YN, we predict an indirect band gap (EΓ−Xg) of 1.09 (LDA) and 1.15 eV (GGA). We also calculated the equilibrium lattice constants, the bulk moduli (Bo), effective masses, and elastic constants for both systems. Our calculated values are in excellent agreement with experimental ones where the latter are available

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“…Correlative IR/SMLM studies are expected to become significantly more approachable with new benchtop light-sources that are brighter than traditional Globars and thus deliver better signal-to-noise faster allowing for nonsynchrotron live-cell experiments 52,53 . Furthermore, robust water absorption correction 54 will allow for examination of the Amide I band and better elucidation of changes to protein composition.…”

Section: Discussionmentioning

confidence: 99%

Correlative Synchrotron Fourier Transform Infrared Spectroscopy and Single Molecule Super Resolution Microscopy for the Detection of Composition and Ultrastructure Alterations in Single Cells

Whelan

Bell

2015

ACS Chem. Biol.

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Single molecule localization microscopy (SMLM) and synchrotron Fourier transform infrared (S-FTIR) spectroscopy are two techniques capable of elucidating unique and valuable biological detail. SMLM provides images of the structures and distributions of targeted biomolecules at spatial resolutions up to an order of magnitude better than the diffraction limit, whereas IR spectroscopy objectively measures the holistic biochemistry of an entire sample, thereby revealing any variations in overall composition. Both tools are currently applied extensively to detect cellular response to disease, chemical treatment, and environmental change. Here, these two techniques have been applied correlatively at the single cell level to probe the biochemistry of common fixation methods and have detected various fixation-induced losses of biomolecular composition and cellular ultrastructure. Furthermore, by extensive honing and optimizing of fixation protocols, many fixation artifacts previously considered pervasive and regularly identified using IR spectroscopy and fluorescence techniques have been avoided. Both paraformaldehyde and two-step glutaraldehyde fixation were identified as best preserving biochemistry for both SMLM and IR studies while other glutaraldehyde and methanol fixation protocols were demonstrated to cause significant biochemical changes and higher variability between samples. Moreover, the potential complementarity of the two techniques was strikingly demonstrated in the correlated detection of biochemical changes as well as in the detection of fixation-induced damage that was only revealed by one of the two techniques.

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Ultrafast infrared chemical imaging of live cells

Cited by 27 publications

References 10 publications

Infrared Spectroscopic Imaging: The Next Generation

Infrared Spectroscopic Imaging: The Next Generation

Electronic, structural, and elastic properties of metal nitrides XN (X = Sc, Y): A first principle study

Correlative Synchrotron Fourier Transform Infrared Spectroscopy and Single Molecule Super Resolution Microscopy for the Detection of Composition and Ultrastructure Alterations in Single Cells

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