Ultrafast Real-Time PCR in Photothermal Microparticles

Kim, Bong Kyun; Lee, Sang‐Ah; Park, Minju; Jeon, Eui Ju; Kim, Mi Jung; Kim, Jung Min; Kim, Heesuk; Jung, Seungwon; Kim, Sang Kyung

doi:10.1021/acsnano.2c07017

Cited by 25 publications

(15 citation statements)

References 43 publications

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“…Since multiple cycles and two or three discrete temperatures are required for the amplification of nucleic acids, to reduce the thermocycling rate has become the key to achieving fast/ultrafast PCR. Nanomaterials with excellent photothermal capabilities have been explored to accelerate heating and cooling processes through volumetric heating and efficient heat transfer. ,− A Au-nanorod-based PCR system has been reported for the rapid detection of bacterial cells (Figure a) . The procedures of the bacteria lysis and DNA amplification are integrated into one step to reduce the cycling time.…”

Section: Applicationsmentioning

confidence: 99%

“…On the other hand, photothermal nanomaterials have exhibited vast perspectives in biomedical areas because of the remote control of heating with high selectivity and spatial accuracy. Numerous applications and techniques based on the photothermal effect have been developed into photothermal therapy (PTT), ,, drug delivery, ,, the polymerase chain reaction (PCR), − and even the fight against coronavirus disease 2019 (COVID-19). − …”

Section: Introductionmentioning

confidence: 99%

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Photothermal Nanomaterials: A Powerful Light-to-Heat Converter

et al. 2023

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All forms of energy follow the law of conservation of energy, by which they can be neither created nor destroyed. Light-to-heat conversion as a traditional yet constantly evolving means of converting light into thermal energy has been of enduring appeal to researchers and the public. With the continuous development of advanced nanotechnologies, a variety of photothermal nanomaterials have been endowed with excellent light harvesting and photothermal conversion capabilities for exploring fascinating and prospective applications. Herein we review the latest progresses on photothermal nanomaterials, with a focus on their underlying mechanisms as powerful light-to-heat converters. We present an extensive catalogue of nanostructured photothermal materials, including metallic/semiconductor structures, carbon materials, organic polymers, and twodimensional materials. The proper material selection and rational structural design for improving the photothermal performance are then discussed. We also provide a representative overview of the latest techniques for probing photothermally generated heat at the nanoscale. We finally review the recent significant developments of photothermal applications and give a brief outlook on the current challenges and future directions of photothermal nanomaterials.

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Section: Applicationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Photothermal Nanomaterials: A Powerful Light-to-Heat Converter

et al. 2023

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“…By combining traditional detection methods, such as polymerase chain reaction (PCR) and agglutination reaction with nanomaterials, bacteria can be detected quickly and conveniently. For instance, photonic PCR is a reliable diagnostic tool for rapid POCT, which utilizes photothermal nanomaterials as heating elements to reduce the power consumption of the reaction and improve the photothermal conversion 79 . Lectin‐functionalized chitosan nanoparticles can be entrapped by crystal violet, resulting in macroscopic aggregates that can be used to detect clinical bacterial infections 50 …”

Section: Nanomaterials and Microbial Diagnosismentioning

confidence: 99%

“…For instance, photonic PCR is a reliable diagnostic tool for rapid POCT, which utilizes photothermal nanomaterials as heating elements to reduce the power consumption of the reaction and improve the photothermal conversion. 79 Lectinfunctionalized chitosan nanoparticles can be entrapped by crystal violet, resulting in macroscopic aggregates that can be used to detect clinical bacterial infections. 50 Microfluidic technology integrates various types of units in a chip, and their small analysis platform, short detection time, and low sample consumption make them widely used in various bacterial detection.…”

Section: Poct Devicesmentioning

confidence: 99%

Applications of nanomaterials as treatments and diagnostic biosensors in microbial infections

Zou,

Tao,

et al. 2023

MedComm – Biomaterials and Applications

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Microbial infection is a major medical problem that seriously threatens public health. The abuse of antibiotics that help evolve the emergence of new drug‐resistance mechanisms has led to the wide‐spread and fast expansion of drug‐resistant bacteria, ultimately evolving into superbugs. This significantly impairs the timely and effective treatment of infections, thus threatening global human well‐being. Not all are pessimistic. Nanomaterials have emerged as an innovative choice. Due to their unique physical and chemical properties, superior bactericidal effects, and high biocompatibility, nanomaterials may help eradicate drug‐resistant bacteria to achieve complete remission of infectious diseases. As biological materials, nanomaterials can also improve the efficacy of existing drugs and treatments and even facilitate diagnostic efficiency. In this review, we aim to comprehensively summarize the antibacterial properties of different kinds of nanomaterials and their applications in other spheres related to treating infectious diseases (targeted therapy, phototherapy, vaccine development, and microbial diagnosis). We highlight the latest advances of nanomaterials in treating infectious diseases in different body systems. Finally, we conclude by discussing the weaknesses of currently available materials and unresolved scientific problems, which may provide insights into the development of approved agents that adequately overcome the notorious drug resistance and thereby provide unprecedented discoveries to improve treatments of the most severe bacterial infections.

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“…In addition, nanoparticle-loaded hydrogels have a wide range of other applications in the biomedical field. They can be used as a reaction platform for qPCR [ 39 ], or as a method for continuous, extreme lubrication of hydrogels in applications ranging from tissue engineering to clinical diagnostics [ 40 ]. They can also be used as drug carriers for the treatment of bone infections caused by bacteria [ 41 ].…”

Section: Introductionmentioning

confidence: 99%

Essential Oil Nanoemulsion Hydrogel with Anti-Biofilm Activity for the Treatment of Infected Wounds

et al. 2023

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The formation of a bacterial biofilm on an infected wound can impede drug penetration and greatly thwart the healing process. Thus, it is essential to develop a wound dressing that can inhibit the growth of and remove biofilms, facilitating the healing of infected wounds. In this study, optimized eucalyptus essential oil nanoemulsions (EEO NEs) were prepared from eucalyptus essential oil, Tween 80, anhydrous ethanol, and water. Afterward, they were combined with a hydrogel matrix physically cross-linked with Carbomer 940 (CBM) and carboxymethyl chitosan (CMC) to prepare eucalyptus essential oil nanoemulsion hydrogels (CBM/CMC/EEO NE). The physical-chemical properties, in vitro bacterial inhibition, and biocompatibility of EEO NE and CBM/CMC/EEO NE were extensively investigated and the infected wound models were proposed to validate the in vivo therapeutic efficacy of CBM/CMC/EEO NE. The results showed that the average particle size of EEO NE was 15.34 ± 3.77 nm with PDI ˂ 0.2, the minimum inhibitory concentration (MIC) of EEO NE was 15 mg/mL, and the minimum bactericidal concentration (MBC) against S. aureus was 25 mg/mL. The inhibition and clearance of EEO NE against S. aureus biofilm at 2×MIC concentrations were 77.530 ± 7.292% and 60.700 ± 3.341%, respectively, demonstrating high anti-biofilm activity in vitro. CBM/CMC/EEO NE exhibited good rheology, water retention, porosity, water vapor permeability, and biocompatibility, meeting the requirements for trauma dressings. In vivo experiments revealed that CBM/CMC/EEO NE effectively promoted wound healing, reduced the bacterial load of wounds, and accelerated the recovery of epidermal and dermal tissue cells. Moreover, CBM/CMC/EEO NE significantly down-regulated the expression of two inflammatory factors, IL-6 and TNF-α, and up-regulated three growth-promoting factors, TGF-β1, VEGF, and EGF. Thus, the CBM/CMC/EEO NE hydrogel effectively treated wounds infected with S. aureus, enhancing the healing process. It is expected to be a new clinical alternative for healing infected wounds in the future.

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Ultrafast Real-Time PCR in Photothermal Microparticles

Cited by 25 publications

References 43 publications

Photothermal Nanomaterials: A Powerful Light-to-Heat Converter

Photothermal Nanomaterials: A Powerful Light-to-Heat Converter

Applications of nanomaterials as treatments and diagnostic biosensors in microbial infections

Essential Oil Nanoemulsion Hydrogel with Anti-Biofilm Activity for the Treatment of Infected Wounds

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