Ultralow power trapping and fluorescence detection of single particles on an optofluidic chip

Kühn, Sergei; Phillips, Brian S.; Lunt, Evan J.; Hawkins, Aaron R.; Schmidt, Holger

doi:10.1039/b915750f

“…Such miniaturized analytical devices are applied in a wide range of scientific and technological fields, for example, biotechnology [350], medical research [351][352][353], centralized laboratory analysis, POCT, and pharmaceutical high-throughput screening (HTS) (see Section 6.2.2.8). Optical systems are integrated advantageously, for example, as sensors [354] or for the manipulation [355] of cells and particles. In contrast to microfluidic systems, HTS is a highly parallelized automatic method for liquid handling.…”

Section: Optical Biosensors Biochips Miniaturized Analytical Systemsmentioning

confidence: 99%

Instruments in Biotechnology and Medicine

Voss¹,

Feller²,

Beckmann³

2012

Handbook of Biophotonics

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The sections in this article are Photonic Instruments in Medicine Introduction Optical Window of Cells and Tissues Laser Light–Matter Interaction as a Function of Intensity Photochemical Treatment of Tissues Thermal Processes in Light–Matter Interaction Nonlinear Processes in Light–Matter Interaction (Not to Be Confused with Nonlinear Optics) Minimally Invasive Diagnostics – Endoscopy History Types and Components of Endoscopes Advantages and Disadvantages of Endoscopy Application Fields Combination of Standard White Light Endoscopic with Other Diagnostic Methods Some Trends in Endoscopy Noninvasive Diagnostics – Photoplethysmography Introduction Characterization of the PPG Signal Signal Acquisition Clinical Applications Summary Noninvasive Diagnostics – Ophthalmology Introduction Corneal Topography Perimetry Optical Biometry Retinal Imaging and Glaucoma Diagnostics Fluorescein Angiography Wavefront Analysis In Vitro Diagnostics – Microscopy Light Microscope Fluorescence Microscope New Fluorescence Microscope Techniques Confocal Microscopy Raman Microscopy In Vitro Diagnostics – Digital Slides and Virtual Microscopy In Vitro Diagnostics – Optical Methods in Clinical Analysis System Optical Spectroscopy of Blood and Urine Components Optical Spectroscopy of Tissues and Vessels Optical Spectroscopy of Agglutination and Precipitation – Nephelometry, Turbidimetry In Vitro Diagnostics – Blood Gas Pressure Measurements Optical Detection of p O 2 in Blood Optical Detection of p CO 2 in Blood Optical Detection of Further Monitoring Parameters in Intensive Care (Temperature, p H ) In Vitro Diagnostics – Photoacoustic Spectroscopy Therapy Introduction Laser Therapy in Ophthalmology – LASIK (Refraction Correction by Cornea Treatment) Photonic Instruments in Biotechnology Introduction Selected Analytical Methods Introduction Reflectometric Interference Spectroscopy ( RIf S ) Surface Plasmon Resonance ( SPR ) Fluorescence Methods Advanced Microscopic Techniques (Selection) Enzyme‐Linked Immunosorbent Assay ( ELISA ) Optical Biosensors, Biochips, Miniaturized Analytical Systems High‐Throughput Screening ( HTS ) Sample Preparation: Flow Injection Analysis Fermentation as a Central Process of Biotechnology General Aspects Fermentation Control Photobioreactors Optical Trap/Optical Tweezers Basic Scheme of Laser Tweezers and Micromanipulation Applications of Optical Tweezers

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“…Recently, the emergence of microfluidics has led to the realization of on-chip biological/ chemical assays. Fluorescence excitation and detection have also been integrated into a chip to make the whole system cost-effective and portable (Chabinyc et al 2001;Fonseca et al 2009;Kuhn et al 2010;Qi et al 2002;Tung et al 2004). To improve the performance of the fluorescence excitation and modify the light properties, a single solid-interface lens was integrated into microfluidic network (Camou et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Multi-functional, optofluidic, in-plane, bi-concave lens: tuning light beam from focused to divergent

Song

¹

,

Nguyen

²

,

Yap

³

et al. 2010

Microfluid Nanofluid

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The miniaturization and integration of in-plane micro-lenses into microfluidic networks for improving fluorescence detection has been widely investigated recently. This article describes the design and demonstration of an optofluidic in-plane bi-concave lens to perform both light focusing and diverging. The concave lens is hydrodynamically formed in a rectangular chamber with a liquid core liquid cladding (L 2 ) configuration. In the focusing mode, an auxiliary cladding stream is introduced to sandwich the L 2 configuration for protecting the light rays from scattering at the rough chamber wall. In the diverging mode, the auxiliary cladding liquid changes its role from avoiding light-scattering to being the lowrefractive-index cladding of the lens. The focal length in the focusing mode and the divergent angle of light beam in the diverging mode can be tuned by adjusting the flow rate ratio between core and cladding streams.

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“…But most of the detection systems remained aloof from the microchannel as close monitoring was found to be less feasible. Hence the development of on-chip methods for the investigation of physical, chemical and biological processes is receiving rapidly increasing attention [3,5,6]. Image fiber based probe system will help in interrogation of remote sites due to its flexibility and miniature size [10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“…Micro-fluidic devices have found an exquisite position in the biomedical technology in recent decades due to their successful implementation in the development of DNA chips, lab-on-a-chip and optofluidic technologies [1][2][3][4][5][6][7][8][9]. Micro-channels have been implemented as pseudo blood vessels with confocal Particle Image Velocimetry (micro-PIV) as the detection system to better understand blood rheology [4,7].…”

Section: Introductionmentioning

confidence: 99%

Real Time Monitoring of Fluorescent Particles in Micro-Channels by High Resolution Dual Modality Probe Imaging

Sathiyamoorthy¹,

Mohankumar²,

Murukeshan³

2011

OPJ

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Imaging of micro particles in micro-fluidic channels is one of the recent thrust areas in research as it provides more flexibility in setup and analysis compared to conventional microscopy. However, a probe based imaging scheme, with achievable high resolutions incorporating multimodal analysis is one of the challenges researchers have been facing. In this context, this paper illustrates a simple dual modality high resolution flexible probe imaging system for imaging applications in micro/optofluidic channels. The proposed system exhibits axial and lateral resolution of about 16 μm and 3.12 μm respectively. This proposed system also exhibits a modulation transfer function (MTF) of about 38.42%. The performance of the system is validated by imaging micro particles in a microchannel and obtaining the fluorescent emission spectrum simultaneously.

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Ultralow power trapping and fluorescence detection of single particles on an optofluidic chip

Cited by 50 publications

References 26 publications

Instruments in Biotechnology and Medicine

Instruments in Biotechnology and Medicine

Multi-functional, optofluidic, in-plane, bi-concave lens: tuning light beam from focused to divergent

Real Time Monitoring of Fluorescent Particles in Micro-Channels by High Resolution Dual Modality Probe Imaging

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