Ultrasensitive analysis of heat shock protein 90α with antibodies orderly arrayed on a novel type of immunoprobe based on magnetic COFs

Zhai, Rui; Gong, Xiaohua; Xie, Jun; Yuan, Yifeng; Xu, Fei; You, Jane; Huang, Zejian; Dai, Xinhua; Zhang, Yangjun; Qian, Xiaohong; Fang, Xiang

doi:10.1016/j.talanta.2018.09.010

Cited by 24 publications

(15 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Outstanding properties of as Fe 3 O 4 @TpBD, such as organized active site, simple synthesis, good reproducibility for detection of biomarkers, arranged placement of conjugated antibodies, and excellent stability, have caused as Fe 3 O 4 @TpBD surpasses other immunoassays in detection of liver cancer biomarkers. [ 161 ]…”

Section: Application Of Cofsmentioning

confidence: 99%

“…[ 173 ] Another magnetic COF immunoprobe captured the heat shock protein 90 α , in human plasma and phosphate buffered saline to help with the diagnosis of liver cancer. [ 161 ] This immunoprobe was composed of materials with higher selectivity and sensitivity to measure the antibodies. [ 161 ]…”

Section: Application Of Cofsmentioning

confidence: 99%

“…[ 161 ] This immunoprobe was composed of materials with higher selectivity and sensitivity to measure the antibodies. [ 161 ]…”

Section: Application Of Cofsmentioning

confidence: 99%

See 2 more Smart Citations

Covalent Organic Frameworks for Biomedical Applications

Esrafili

Wagner

Inamdar

et al. 2021

Adv Healthcare Materials

133

View full text Add to dashboard Cite

Covalent organic frameworks (COFs) are porous organic polymeric materials that are composed of organic elements and linked together by the thermodynamically stable covalent bonds. The applications of COFs in energy sector and drug delivery are afforded because of the desirable properties of COFs, such as high stability, low density, large surface area, multidimensionality, porosity, and high‐ordered crystalline structure expanded. In this review COFs are reviewed, from the perspective of different types of reported COFs, different methods for their synthesis, and their potential applications in the biomedical field. The main goal of this review is to introduce COFs as a biomaterial and to identify specific advantages of different types of COFs that can be exploited for specialized biomedical applications, such as immune engineering.

show abstract

Section: Application Of Cofsmentioning

confidence: 99%

Section: Application Of Cofsmentioning

confidence: 99%

See 1 more Smart Citation

Covalent Organic Frameworks for Biomedical Applications

Esrafili

Wagner

Inamdar

et al. 2021

Adv Healthcare Materials

133

View full text Add to dashboard Cite

show abstract

“…It was shown that HSP70 translocates into the plasma membrane after stress and alters the plasma membrane of acinar cells, allowing substances a size of 70,000 MW to enter the cytosol [14][15][16]. So far, while many techniques have been developed for detection of HSPs in various biological fluids, they are associated with several diseases, such as heart failure [17], seizure-related brain injury [18], or liver cancer [19] and can serve as markers for early clinical screening of depression [20]. Currently accepted methods for the detection of HSPs include enzyme-linked immunosorbent assay (ELISA) [17][18][19]21], immunofluorescence [22], Western blotting [23], qRT-PCR [24], and flow cytometry [21,22,24].…”

Section: Introductionmentioning

confidence: 99%

“…So far, while many techniques have been developed for detection of HSPs in various biological fluids, they are associated with several diseases, such as heart failure [17], seizure-related brain injury [18], or liver cancer [19] and can serve as markers for early clinical screening of depression [20]. Currently accepted methods for the detection of HSPs include enzyme-linked immunosorbent assay (ELISA) [17][18][19]21], immunofluorescence [22], Western blotting [23], qRT-PCR [24], and flow cytometry [21,22,24]. Although these methods have realized the sensitive determination of HSPs, the timeconsuming and complex experimental process, derived from the peculiarities of an assessments of particular disease, makes the use of these techniques for wider applications limited.…”

Section: Introductionmentioning

confidence: 99%

Tethered Lipid Membranes as a Nanoscale Arrangement towards Non-Invasive Analysis of Acute Pancreatitis

et al. 2021

View full text Add to dashboard Cite

Extracellular heat shock proteins (HSPs) mediate immunological functions and are involved in pathologies such as infection, stress, and cancer. Here, we demonstrated the dependence of an amount of HSP70 and HSP90 in serum vs. severity of acute pancreatitis (AP) on a cohort of 49 patients. Tethered bilayer lipid membranes (tBLMs) have been developed to investigate HSPs’ interactions with tBLMs that can be probed by electrochemical impedance spectroscopy (EIS). The results revealed that HSP70 and HSP90 interact via different mechanisms. HSP70 shows the damage of the membrane, while HSP90 increases the insulation properties of tBLM. These findings provide evidence that EIS offers a novel approach for the study of the changes in membrane integrity induced by HSPs proteins. Herein, we present an alternative electrochemical technique, without any immunoprobes, that allows for the monitoring of HSPs on nanoscaled tBLM arrangement in biologics samples such us human urine. This study demonstrates the great potential of tBLM to be used as a membrane based biosensor for novel, simple, and non-invasive label-free analytical system for the prediction of AP severity.

show abstract

Chemical Sensors Based on Covalent Organic Frameworks

Yue,

Ji,

Liu

et al. 2023

Chemistry A European J

View full text Add to dashboard Cite

Covalent organic frameworks (COFs) are a type of crystalline porous polymer composed of light elements through strong covalent bonds. COFs have attracted considerable attention due to their unique designable structures and excellent material properties. Currently, COFs have shown outstanding potential in various fields, including gas storage, pollutant removal, catalysis, adsorption, optoelectronics, and their research in the sensing field is also increasingly flourishing. In this review, we focus on COF‐based sensors. Firstly, we elucidate the fundamental principles of COF‐based sensors. Then, we present the primary application areas of COF‐based sensors and their recent advancements, encompassing gas, ions, organic compounds, and biomolecules sensing. Finally, we discuss the future trends and challenges faced by COF‐based sensors, outlining their promising prospects in the field of sensing.

show abstract

Ultrasensitive analysis of heat shock protein 90α with antibodies orderly arrayed on a novel type of immunoprobe based on magnetic COFs

Cited by 24 publications

References 44 publications

Covalent Organic Frameworks for Biomedical Applications

Covalent Organic Frameworks for Biomedical Applications

Tethered Lipid Membranes as a Nanoscale Arrangement towards Non-Invasive Analysis of Acute Pancreatitis

Chemical Sensors Based on Covalent Organic Frameworks

Contact Info

Product

Resources

About