Ultrasensitive and Visual Electrochemiluminescence Ratiometry Based on a Constant Resistor-Integrated Bipolar Electrode for MicroRNA Detection

Abstract

In this work, a new electrochemiluminescence (ECL) platform was constructed for detecting the prostate cancer marker microRNA-141 (miRNA-141) on a constant resistorintegrated closed bipolar electrode (BPE). It consisted of two reservoirs and a constant resistor, and both ends were connected to the anode of the driving electrode and the cathode of BPE. The cathode of BPE was modified with boron nitride quantum dots (BNQDs), and the anode reservoir was the [Ru(bpy) 3 ](PF 6 ) 2 / TPrA system. After introducing a certain amount of hairpin DNA 3 (H3) and ferrocene-labeled single-stranded DNA (Fc-ssDNA) on the surface of the BNQDs, the ECL emission signal of the BNQDs was difficult to be observed by the naked eye, while [Ru(bpy) 3 ]-(PF 6 ) 2 emitted a strong and visible ECL signal. In the presence of the target, bipedal DNA assembled by catalytic hairpin assembly (CHA) took away the Fc-ssDNA and the ECL intensity of the BNQDs was enlarged, and as the concentration of miRNA-141 increased to the cutoff value, yellow-green light was visible by the naked eye. Meanwhile, the red emission signal of [Ru(bpy) 3 ](PF 6 ) 2 /TPrA became weakened. Thus, an ultrasensitive "color switch" ECL biosensor for detection of miRNA-141 was constructed and endowed with a wide linear range from 10 −17 to 10 −7 M and a detection limit of 10 −17 M (S/N = 3). This study provides the potential for investigating portable devices in the detection of lowconcentration nucleic acids.

Pyrene-Based Hydrogen-Bonded Organic Frameworks as New Emitters with Porosity- and Aggregation-Induced Enhanced Electrochemiluminescence for Ultrasensitive MicroRNA Assay

“…MicroRNA-141 (miRNA-141) has been considered an appealing biomarker for cancers since its abnormal expression in various types of human diseases, such as the cancers of the prostate, cervical, and breast . Therefore, it is of great significance to develop sensitive and efficient detection methods for miRNA-141.…”

Gold Nanocluster Probe-Based Electron-Transfer-Mediated Electrochemiluminescence Sensing Strategy for an Ultrasensitive Copper Ion Detection

“…19−21 Afterward, quantum dots have been used to explore the ET-ECL system for wider applications. 22,23 However, the quencher is almost limited to ferrocene, which greatly hinders its application. Therefore, exploring a novel and high-efficiency ET-ECL system is urgently needed.…”

“…In addition to photoinduced electron transfer, the ET reaction has also proved to be a capable ECL-sensing strategy. Landers group first report the ET-based ECL mechanism (ET-ECL) between Ru­(bpy) 3 2+* and ferrocenium, which has been used as a common ET-ECL model for ECL-sensing applications. − Afterward, quantum dots have been used to explore the ET-ECL system for wider applications. , However, the quencher is almost limited to ferrocene, which greatly hinders its application. Therefore, exploring a novel and high-efficiency ET-ECL system is urgently needed.…”

Electrochemiluminescence devices for point-of-care testing

“…(D) Structure and sensing result of the naked eye direct-reading ECL platform. 45 Copyright 2022, American Chemical Society. (E) Sensing diagram of the self-reinforcing solid-state ECL system.…”