Ultrasensitive aptamer-functionalized Cu-MOF fluorescent nanozyme as an optical biosensor for detection of C-reactive protein

Ali, Gona K.; Omer, Khalid M.

doi:10.1016/j.ab.2022.114928

Cited by 54 publications

(25 citation statements)

References 53 publications

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“…With the rapid development of nanotechnology, scientists have synthesized nanozymes that not only have the unique physical and chemical properties of nanomaterials but also have enzyme catalytic activity. Nanozymes have a high surface area and good optical and electrical properties, so they are widely used in biosensing technology [48,49]. Compared with natural enzymes and traditional artificial enzymes, nanozymes are simple to prepare, easy to modify, and resistant to acids and bases.…”

Section: Enzyme-catalyze Signal Amplificationmentioning

confidence: 99%

Signal Amplification-Based Biosensors and Application in RNA Tumor Markers

Zhang

Gan

et al. 2023

Sensors

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Tumor markers are important substances for assessing cancer development. In recent years, RNA tumor markers have attracted significant attention, and studies have shown that their abnormal expression of post-transcriptional regulatory genes is associated with tumor progression. Therefore, RNA tumor markers are considered as potential targets in clinical diagnosis and prognosis. Many studies show that biosensors have good application prospects in the field of medical diagnosis. The application of biosensors in RNA tumor markers is developing rapidly. These sensors have the advantages of high sensitivity, excellent selectivity, and convenience. However, the detection abundance of RNA tumor markers is low. In order to improve the detection sensitivity, researchers have developed a variety of signal amplification strategies to enhance the detection signal. In this review, after a brief introduction of the sensing principles and designs of different biosensing platforms, we will summarize the latest research progress of electrochemical, photoelectrochemical, and fluorescent biosensors based on signal amplification strategies for detecting RNA tumor markers. This review provides a high sensitivity and good selectivity sensing platform for early-stage cancer research. It provides a new idea for the development of accurate, sensitive, and convenient biological analysis in the future, which can be used for the early diagnosis and monitoring of cancer and contribute to the reduction in the mortality rate.

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Section: Enzyme-catalyze Signal Amplificationmentioning

confidence: 99%

Signal Amplification-Based Biosensors and Application in RNA Tumor Markers

Zhang

Gan

et al. 2023

Sensors

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“…In addition to providing routes for the mass movement of reactants and products, the large surface area of MOFs facilitates the appropriate exposure of active areas. These characteristics make MOFs very well-suited for heterogeneous catalysis. − Recently, MOFs with an inherent enzymelike activity have been designed for the detection of TAC, such as CuBDC@Fe(III)-ART NPs, Fe 3 O 4 @Au–MOFs, and Tα-MOF . Although organic linkers with terminal carboxylate groups are frequently used in MOF synthesis, only a few strontium-based compounds have been identified to a considerable extent.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Peroxidase-Mimic Catalytic Activity via Cerium Doping of Strontium-Based Metal–Organic Frameworks with Design of a Smartphone-Based Sensor for On-Site Salivary Total Antioxidant Capacity Detection in Lung Cancer Patients

Alshatteri

Ali

Omer

2023

ACS Appl. Mater. Interfaces

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The development of artificial nanozymes with superior catalytic performance and excellent stability has been a long-standing objective for chemists. The total antioxidant capacity (TAC) is one of the most important bioanalytical measures of oxidative stress in the body. The present work aims to develop a smartphone-assisted visual detection sensor using cerium-doped strontium-based metal–organic frameworks (Ce-SrMOFs) as peroxidase-like nanozymes for the rapid, low-cost, on-site detection of TAC. The pristine SrMOF functioned as a peroxidase nanozyme, and its enzymatic activity was enhanced after doping it with Ce(IV) ions because of the multivalent nature and synergistic impact of the heteroatoms. The Ce-SrMOFs were sensitive to the single electron transfer and hydrogen atom transfer processes, which implies that the Ce-SrMOFs can serve as an ideal nanozyme candidate for TAC analysis. The investigated mechanism revealed that •OH is the most active oxygen species for the peroxidase-like activity. The Ce-SrMOFs exhibited a strong affinity for 3,3′,5,5′-tetramethylbenzidine (TMB) and H2O2, with K m values of 0.082 and 0.427 mM, which are 5.29- and 8.67-fold lower than those of horseradish peroxidase (HRP), respectively. The Ce-SrMOFs were used for the detection of ascorbic acid, cysteine, and glutathione, with limits of detection of 44, 53, and 512 nM, respectively. The proposed method proved effective in measuring the TAC in saliva samples from lung cancer patients, thereby yielding results with satisfactory precision and accuracy.

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“…Metal–organic frameworks (MOFs) are porous crystalline materials prepared by metal ions/clusters and organic ligands linking by coordination bonds. − MOFs have been intensively investigated for biochemical applications due to their distinctive physicochemical features including high thermal stability, high surface area, porosity, presence of cavities and functionality. − Many optical-based applications require stable, durable, and effective luminescent materials. , Luminescent metal–organic frameworks (LMOFs), as a part of the metal–organic frameworks (MOFs) family, are gaining great attention recently due to their enormous applications in bio(chemical) sensing and bioimaging. − Although many functionalizations of MOFs have been reported to prepare LMOFs, such as using fluorescent linker in MOF and/or insertion of luminophores into the MOF structure such as nanoclusters, carbon dot, and fluorescent dyes, the fabrication of MOFs with intrinsic luminescence is more practical and valuable for many applications due to stability of the MOFs and low-cost precursors . In the literature, for the construction of intrinsic LMOFs, lanthanide elements, such as Eu 3+ and Tb 3+ are used. ,,, Such elements have higher costs, potential supply shortages, and adverse effects on the environment.…”

Section: Introductionmentioning

confidence: 99%

Intrinsic Dual-State Emission Zinc-Based MOF Rodlike Nanostructures with Applications in Smartphone Readout Visual-Based Detection for Tetracycline: MOF-Based Color Tonality

Mohammed Ameen,

Qasim,

Alhasan

et al. 2023

ACS Appl. Mater. Interfaces

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Dual-state emitters (DSEs) are entities that exhibit fluorescence in both the solution and solid state, which open up a wide range of possibilities for their utilization in various fields. The development of detection platforms using intrinsic luminescent metal–organic frameworks (LMOFs) is highly desirable for a variety of applications. DSE MOFs as a subclass of intrinsic LMOFs are highly attractive due to no need for encapsulation/functionalization by fluorophores and/or using luminescent linkers. Herein, a highly stable intrinsic dual-state blue emission (λem = 425 nm) zinc-based MOF with rodlike nanostructures (denoted as UoZ-2) was synthesized and characterized. To the best of our knowledge, intrinsic DSE of Zn-MOFs with blue emission in the dispersed form in solution and solid-state fluorescence have not been reported yet. When tetracycline (TC) was added, a continuous color evolution from blue to greenish-yellow with dramatic enhancement was observed due to aggregation induced emission (AIE). Thus, a sensitive ratiometry-based visual detection platform, in solution and on paper independently, was designed for detection of TC exploiting the DSE and AIE properties of UoZ-2 alone and UoZ-2:TC. The detection limit was estimated to be 4.5 nM, which is considered to be one of the most sensitive ratiometric fluorescent probes for TC sensing. The ratiometry paper-based UoZ-2 sensor displays a reliable TC quantitative analysis by recognizing RGB values in the on-site TC detection with satisfactory recoveries.

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Ultrasensitive aptamer-functionalized Cu-MOF fluorescent nanozyme as an optical biosensor for detection of C-reactive protein

Cited by 54 publications

References 53 publications

Signal Amplification-Based Biosensors and Application in RNA Tumor Markers

Signal Amplification-Based Biosensors and Application in RNA Tumor Markers

Enhanced Peroxidase-Mimic Catalytic Activity via Cerium Doping of Strontium-Based Metal–Organic Frameworks with Design of a Smartphone-Based Sensor for On-Site Salivary Total Antioxidant Capacity Detection in Lung Cancer Patients

Intrinsic Dual-State Emission Zinc-Based MOF Rodlike Nanostructures with Applications in Smartphone Readout Visual-Based Detection for Tetracycline: MOF-Based Color Tonality

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