Ultrasensitive colorimetric detection of protein by aptamer–Au nanoparticles conjugates based on a dot-blot assay

Wang, Yuling; Li, Dan; Ren, Wen; Liu, Zuojia; Dong, Shaojun; Wang, Erkang

doi:10.1039/b801055b

Cited by 131 publications

(67 citation statements)

References 36 publications

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“…2 Nevertheless, the high nonspecific background encountered in complex media can be efficiently diminished by changing the detection format with double-site interaction, in particular, using sandwich assays. [3][4][5][6][7][8][9][10][11][12][13] Alternatively, the proximity between two aptamers that bind different thrombin epitopes has been exploited to collect a specific signal of the target. [14][15][16][17] Enhanced sensitivity and reduced reaction time have been achieved with bivalent aptamer structures in which distinct aptamers are conjugated with an optimal linker, 18,19 or co-conjugated to the sensor surface with an optimal density.…”

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confidence: 99%

Self-bending symmetric cusp beams

Gong

Liu

Ren

et al. 2015

Applied Physics Letters

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A type of self-bending symmetric cusp beams with four accelerating intensity maxima is theoretically and experimentally presented. Distinguished from the reported regular polygon beams, the symmetric cusp beams simultaneously exhibit peculiar features of natural autofocusing and self-acceleration during propagation. Further, such beams take the shape of a fine longitudinal needle-like structure at the focal region and possess the strong ability of self-healing over obstacles. All these intriguing properties were verified experimentally. Particularly, the spatial profile of the reconstructed beam exhibits spatially sculpted optical structure with four siamesed curved arms. Thus, we anticipate that the structured beam will benefit optical guiding and optofluidics in surprising ways.

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confidence: 99%

Self-bending symmetric cusp beams

Gong

Liu

Ren

et al. 2015

Applied Physics Letters

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“…[24] In aptasensors, aptamers have commonly served as the capture molecules to detect proteins. [25,26] Among different methods of fluorescence, [27] EC, [28] ECL [29] and colorimetry, [30,31] ECL has attracted substantial attention in the development of aptasensors because it is highly sensitive and inexpensive, and uses simple instruments. Traditionally, ECL aptasensors have used diverse luminophore-labeled aptamers, [32][33][34] which are difficult to obtain.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive and Signal‐on Electrochemiluminescence Aptasensor Using the Multi‐tris(bipyridine)ruthenium(II)‐β‐cyclodextrin Complexes

Zhao

Chen

et al. 2014

Chin. J. Chem.

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An ultrasensitive and signal-on electrochemiluminescence (ECL) aptasensor to detect target protein (thrombin or lysozyme) was developed using the host-guest recognition between a metallocyclodextrin complex and single-stranded DNA (ss-DNA). The aptasensor uses both the photoactive properties of the metallocyclodextrins named multi-tris(bipyridine)ruthenium(II)-β-cyclodextrin complexes and their specific recognition with ss-DNA, which amplified the ECL signal without luminophore labeling. After investigating the ECL performance of different multi-tris(bipyridine)ruthenium(II)-β-cyclodextrin (multi-Ru-β-CD) complexes, tris-tris(bipyridine)-ruthenium(II)-β-cyclodextrin (tris(bpyRu)-β-CD) was selected as a suitable host molecule to construct an atasensor. First, double-stranded DNA (ds-DNA) formed by hybridization of the aptamer and its target DNA was attached to a glassy carbon electrode via coupling interaction, which showed low ECL intensity with 2-(dibutylamino) ethanol (DBAE) as coreactant, because of the weak recognition between ds-DNA and tris(bpyRu)-β-CD. Upon addition of the corresponding protein, the ECL intensity increased when target ss-DNA was released because of the higher stability of the aptamer-protein complex than the aptamer-DNA one. A linear relationship was observed in the range of 0.01 pmol/L to 100 pmol/L between ECL intensity and the logarithm of thrombin concentrations with a limited detection of 8.5 fmol/L (S/N＝3). Meanwhile, the measured concentration of lysozyme was from 0.05 pmol/L to 500 pmol/L and the detection limit was 33 fmol/L (S/N＝3). The investigations of proteins in human serum samples were also performed to demonstrate the validity of detection in real clinical samples. The simplicity, high sensitivity and specificity of this aptasensor show great promise for practical applications in protein monitoring and disease diagnosis.

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“…Generell wird bei den markerfreien Methoden über einen Signalwandler, auf dessen Oberfläche Liganden zum Fangen der zu detektierenden Proteine immobilisiert sind, die Bindung der Proteine in ein messbares Signal umgewandelt. Dieses kann elektrochemisch, kolorimetrisch oder optisch durchgeführt werden [7][8][9] …”

Section: Introductionunclassified

Plattformtechnologie für die mobile, markerfreie Proteindetektion

Jahns

Neustock

Paulsen

et al. 2017

Tm - Technisches Messen

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ZusammenfassungWir präsentieren eine Plattformtechnologie zur mobilen, markerfreien Detektion von mehreren Proteinen gleichzeitig. Ein photonischer Kristall, der mit Liganden lokal funktionalisiert ist, dient hier als Sensor. Über ein kompaktes, Kamera-basiertes Messsystem wird die Proteinanbindung an die Sensoroberfläche in ein Intensitätssignal umgewandelt, über dessen Amplitude die Proteinkonzentration bestimmt werden kann. Um die Detektionsgrenze dieser Technologie weiter zu verbessern, werden hier photonische Kristalle mit einer multiperiodischen und aperiodischen Gitterstruktur simulativ und experimentell untersucht. Dafür werden die Gesamtempfindlichkeit und die Resonanzgüte jeder Struktur bestimmt und mit der bisher verwendeten monoperiodischen Struktur verglichen. Es konnte festgestellt werde, dass die Resonanzgüte von mono-über multi-bis aperiodisch sich deutlich verbessert. Eine Steigerung der Gesamtempfindlichkeit konnte nicht festgestellt werden. Jedoch konnte anhand von Analysen der elektrischen Feldverteilung innerhalb der verschiedenen Strukturen beobachtet werden, dass die Modenausbreitungen in den aperiodischen Strukturen stark lokalisiert wird und die -Funktionalisierung repräsentiert, liegt. Diese lokale Resonanzausbildung konnte zudem bereits in ersten experimentellen Untersuchungen bestätigt werden.

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Ultrasensitive colorimetric detection of protein by aptamer–Au nanoparticles conjugates based on a dot-blot assay

Cited by 131 publications

References 36 publications

Self-bending symmetric cusp beams

Self-bending symmetric cusp beams

Ultrasensitive and Signal‐on Electrochemiluminescence Aptasensor Using the Multi‐tris(bipyridine)ruthenium(II)‐β‐cyclodextrin Complexes

Plattformtechnologie für die mobile, markerfreie Proteindetektion

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