Ultrasensitive detection of microRNA using a bismuthene-enabled fluorescence quenching biosensor

Xue, Tao; Bongu, Sudhakara Reddy; Huang, Hao; Liang, Weiyuan; Wang, Yiduo; Zhang, Feng; Liu, Zhongyuan; Zhang, Yupeng; Zhang, Han; Cui, Xiaoqiang

doi:10.1039/d0cc01004a

Cited by 57 publications

(39 citation statements)

References 41 publications

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“…The limit of detection (LOD) was estimated to be 0.2 pM according to the 3σ rule, which was comparable to some previously reported fluorescence assays for miRNA. 26 − 28 Moreover, the analytical performance of the proposed method was also compared to other reported methods (Table S2, Supporting Information ). The high sensitivity and the low background might attribute to the dual-signal amplification procedure and the high fidelity of Endo IV in specifically cleaving the TaqMan probe in dsDNA.…”

Section: Results and Discussionmentioning

confidence: 99%

Dual-Signal Amplification Strategy for Sensitive MicroRNA Detection Based on Rolling Circle Amplification and Enzymatic Repairing Amplification

et al. 2020

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MicroRNAs (miRNAs) play crucial regulatory roles as post-transcriptional regulators for gene expression and serve as promising biomarkers for diagnosis and prognosis of diseases. Herein, a dual-signal amplification method has been developed for sensitive and selective detection of miRNA based on rolling circle amplification (RCA) and enzymatic repairing amplification (ERA) with low nonspecific background. This strategy designs a padlock probe that can be cyclized in the presence of target miRNA to initiate the RCA reaction, after which the TaqMan probes that are complementary to the RCA products can be cyclically cleaved to produce obvious fluorescence signals with the help of endonuclease IV (Endo IV). Attributed to the dual-signal amplification procedure and the high fidelity of Endo IV, the RCA–ERA method allows quantitative detection of miR-21 in a dynamic range from 2 pM to 5 nM with a low background signal. Moreover, it has the ability to discriminate single-base difference between miRNAs and shows good performance for miRNA detection in complex biological samples. The results demonstrate that the RCA–ERA assay holds a great promise for miRNA-based diagnostics.

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Section: Results and Discussionmentioning

confidence: 99%

Dual-Signal Amplification Strategy for Sensitive MicroRNA Detection Based on Rolling Circle Amplification and Enzymatic Repairing Amplification

et al. 2020

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“…In 2020, Xue et al. [ 64 ] reported that ultrathin Bi nanosheets were successfully fabricated by an LPE technique in NMP solvent. The AFM topography of typical Bi nanosheets shows that the lateral dimensions of the as‐fabricated Bi nanosheets are more than 800 nm, and the thickness is quite thin (≈1 nm) ( Figure a), consistent with the previously reported bilayer thickness of Bi nanosheets.…”

Section: Synthesis Of Bi Nanostructuresmentioning

confidence: 99%

“…Reproduced with permission. [ 64 ] Copyright 2020, The Royal Society of Chemistry. d) SEM image, e) TEM image, and f) HRTEM image of Bi nanosheets prepared by in situ electrocatalytic topotactic transformation of bismuth iodide oxide nanosheets.…”

Section: Synthesis Of Bi Nanostructuresmentioning

confidence: 99%

“…Fluorescent Bi semiconductor nanomaterials have generated great interest for a variety of promising applications, [ 26,64,232–234 ] especially those in biosensors. [ 14,64,235 ] For example, in 2017, an efficient hot‐pressing method was developed by Hussain et al.…”

Section: Properties and Applications Of Bi Nanostructuresmentioning

confidence: 99%

“…[ 93,157,159,235 ] For example, a novel ultrathin bismuthene‐based sensing system for microRNA (miRNA)‐specific detection was developed by Xue et al. [ 64 ] Well‐dispersed and ultrathin 2D bismuthene nanosheets were successfully fabricated by an LPE method in NMP. The schematic illustration of 2D bismuthene nanosheet‐based miRNA detection can be seen in Figure a.…”

Section: Properties and Applications Of Bi Nanostructuresmentioning

confidence: 99%

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Ultrasensitive detection of microRNA using a bismuthene-enabled fluorescence quenching biosensor

Abstract: A novel ultrathin bismuthene-based sensing platform for microRNA (miRNA)-specific detection has been developed.

Cited by 57 publications

References 41 publications

Dual-Signal Amplification Strategy for Sensitive MicroRNA Detection Based on Rolling Circle Amplification and Enzymatic Repairing Amplification

Dual-Signal Amplification Strategy for Sensitive MicroRNA Detection Based on Rolling Circle Amplification and Enzymatic Repairing Amplification

Emerging Mono‐Elemental Bismuth Nanostructures: Controlled Synthesis and Their Versatile Applications

NIR‐II Responsive Inorganic 2D Nanomaterials for Cancer Photothermal Therapy: Recent Advances and Future Challenges

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