Ultrasensitive detection of nonlabelled bovine serum albumin using photothermal optical phase shift detection with UV excitation

Shimizu, Hisashi; Takeda, Shigenori; Mawatari, Kazuma; Kitamori, Takehiko

doi:10.1039/d0an00037j

Cited by 12 publications

(15 citation statements)

References 31 publications

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“…6. 78 In contrast to the setup shown in Fig. 5, the excitation beam is switched from VIS to UV light to detect a wide range of molecules, including proteins, peptides, amino acids, and nucleic acids.…”

Section: Instrumentationmentioning

confidence: 99%

Review of ultrasensitive readout for micro-/nanofluidic devices by thermal lens microscopy

Chen

Shimizu

Kitamori

2021

J. Optical Microsystems

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Thermal lens microscopy (TLM) utilizes the effects, such as changes in the refractive index, caused by heat generated in the sample for the detection of nonfluorescent analytes with at least a hundred-fold enhancement in sensitivity compared with optical absorbance spectrometry. Micro-/nanofluidic devices can provide specificity and sample manipulation capabilities. The integration of these two technologies exposes the potential to attain the holy grail of continuous, real-time, label-free, specific, and ultrasensitive detection, which find applications in environmental monitoring, quality control of chemical manufacturing, single-cell analysis, and biomedicines. Here, we summarize the recent advances in the instrument development and innovative applications, and suggest future directions of research of TLM. © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Instrumentationmentioning

confidence: 99%

Review of ultrasensitive readout for micro-/nanofluidic devices by thermal lens microscopy

Chen

Shimizu

Kitamori

2021

J. Optical Microsystems

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“…The targets of POPS detection are all molecules which have optical absorption in UV/vis range. For example, ~30 protein molecules were detected by the UV-POPS detector as shown in Figure 3 b [ 66 ].…”

Section: Optical Detectionmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

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Advances in Label-Free Detections for Nanofluidic Analytical Devices

Shimizu

Morikawa

2020

Micromachines

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Nanofluidics, a discipline of science and engineering of fluids confined to structures at the 1–1000 nm scale, has experienced significant growth over the past decade. Nanofluidics have offered fascinating platforms for chemical and biological analyses by exploiting the unique characteristics of liquids and molecules confined in nanospaces; however, the difficulty to detect molecules in extremely small spaces hampers the practical applications of nanofluidic devices. Laser-induced fluorescence microscopy with single-molecule sensitivity has been so far a major detection method in nanofluidics, but issues arising from labeling and photobleaching limit its application. Recently, numerous label-free detection methods have been developed to identify and determine the number of molecules, as well as provide chemical, conformational, and kinetic information of molecules. This review focuses on label-free detection techniques designed for nanofluidics; these techniques are divided into two groups: optical and electrical/electrochemical detection methods. In this review, we discuss on the developed nanofluidic device architectures, elucidate the mechanisms by which the utilization of nanofluidics in manipulating molecules and controlling light–matter interactions enhances the capabilities of biological and chemical analyses, and highlight new research directions in the field of detections in nanofluidics.

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“…The experimental results demonstrated that the measurement of the BSA concentration could still be obtained from the drift of the spectral line under interference factors. Recently, the detection of the nonlabelled BSA using photothermal optical phase shift (POPS) detection with ultraviolet (UV) excitation as well as BSA detection by atmospheric pressure plasma jets in matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has been addressed [35,36]. Therefore, the GMLPG demonstrated a great potential for biosensing due to the simple monitoring system, label-free, and comparable LOD.…”

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confidence: 99%

Bovine Serum Albumin Detection by Graphene Oxide Coated Long-Period Fiber Grating

Wang

et al. 2022

Photonic Sens

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A biosensor for bovine serum albumin (BSA) detection by graphene oxide (GO) functionalized micro-taped long-period fiber grating (GMLPG) was demonstrated. The amide bond connected between the GO and BSA enabled the BSA to attach onto the fiber surface, which changed the effective refractive index of the cladding mode and characterized the concentration of the BSA. This real-time monitoring system demonstrated a sensing sensitivity of 1.263 nm/(mg/mL) and a detection limit of 0.043 mg/mL. Moreover, it illustrated superior measurement performance of higher sensitivity in the presence of glucose and urea as the interference, which showed static sensitivities of ∼1.476 nm/(mg/mL) and 1.504 nm/(mg/mL), respectively. The proposed GMLPG demonstrated a great potential for being employed as a sensor for biomedical and biochemical applications.

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Ultrasensitive detection of nonlabelled bovine serum albumin using photothermal optical phase shift detection with UV excitation

Abstract: Ultrasensitive detection of nonlabelled bovine serum albumin is performed in micro/nanofluidic chips using a photothermal optical phase shift (POPS) detection system.

Cited by 12 publications

References 31 publications

Review of ultrasensitive readout for micro-/nanofluidic devices by thermal lens microscopy

Review of ultrasensitive readout for micro-/nanofluidic devices by thermal lens microscopy

Advances in Label-Free Detections for Nanofluidic Analytical Devices

Bovine Serum Albumin Detection by Graphene Oxide Coated Long-Period Fiber Grating

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